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Identification and structure analysis of three tilapia species using microsatellite markers

Published online by Cambridge University Press:  30 October 2009

Song Hong-Mei ,
Bai Jun-Jie ,
Quan Ying-Chun  and
Li Sheng-Jie
Song Hong-Mei
Affiliation:
Pearl River Fisheries Research Institute, Key Laboratory of Tropical and Subtropical Fish Breeding and Cultivation, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong 510380, China College of Aqua-life Science and Technology, Shanghai Fisheries University, Shanghai 200090, China
Bai Jun-Jie*
Affiliation:
Pearl River Fisheries Research Institute, Key Laboratory of Tropical and Subtropical Fish Breeding and Cultivation, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong 510380, China
Quan Ying-Chun
Affiliation:
Pearl River Fisheries Research Institute, Key Laboratory of Tropical and Subtropical Fish Breeding and Cultivation, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong 510380, China
Li Sheng-Jie
Affiliation:
Pearl River Fisheries Research Institute, Key Laboratory of Tropical and Subtropical Fish Breeding and Cultivation, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong 510380, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

Seventeen special loci were selected from 77 microsatellite markers to distinguish three varieties of tilapias, including the six differential loci UNH636, UNH117, UNH172, UNH738, UNH878 and UNH896 in Oreochromis aureus; five differential loci UNH913, UNH907, UNH222, UNH980 and UNH880 in O. niloticus; and six differential loci of UNH876, UNH899, UNH853, UNH932, UNH933 and UNH773 in O. mossambicus. Any one of the 17 loci could amplify particular bands to distinguish one tilapia from the other two. The genetic structure of O. aureus, O. niloticus and O. mossambicus stocks and their phylogenetic relationships were also analysed using these 17 loci. In total 142 alleles were detected, and the average number of alleles per locus was 8.35. Additionally, a clustering analysis was performed based on the result of the Popgen32 software package and phylogenetic trees were constructed by MEGA4 using the unweighted pair group method using arithmetic average (UPGMA). The results showed that the mean value of observed heterozygosity was 0.0941, 0.5490 and 0.2588, the mean value of expected heterozygosity was 0.1089, 0.7230 and 0.1965, and the polymorphism information content was 0.0869, 0.7149 and 0.1643, in O. aureus, O. niloticus and O. mossambicus, respectively. The UPGMA tree demonstrated that O. aureus was more closely related to O. mossambicus than to O. niloticus.

Keywords

Oreochromis aureusOreochromis niloticusOreochromis mossambicusmicrosatellitegermplasm identificationgenetic structure
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 6 , Issue 2 , August 2009 , pp. 119 - 125
Copyright
Copyright © China Agricultural University 2009

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