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Genetic structure analyses of different populations of grass carp (Ctenopharyngodon idella) using the TRAP technique

Published online by Cambridge University Press:  15 June 2007

Zhang Zhi-Wei ,
Cao Zhe-Ming ,
Zhou Jing-Song  and
Wu Ting-Ting
Zhang Zhi-Wei
Affiliation:
Wuxi Fishery College, Nanjing Agricultural University, Wuxi 214081, China Jiangsu Institute of Oceanology and Marine fisheries, Nantong 226007, China
Cao Zhe-Ming
Affiliation:
Freshwater Fisheries Research Center of the Chinese Academy of Fishery Sciences, Wuxi 214081, China
Zhou Jing-Song
Affiliation:
Freshwater Fisheries Research Center of the Chinese Academy of Fishery Sciences, Wuxi 214081, China
Wu Ting-Ting*
Affiliation:
Freshwater Fisheries Research Center of the Chinese Academy of Fishery Sciences, Wuxi 214081, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

Target region amplified polymorphism (TRAP) was used to compare genetic structures among three populations of grass carp (Ctenopharyngodon idella) – one wild and two cultured populations. Seven out of 15 primer combinations produced good amplification patterns and provided 103 amplified loci from the three populations. Numbers of polymorphic loci in the wild population were higher, indicating a decrease in genetic polymorphism in the two cultured populations. Compared with the wild population, only 39.98% loci gene frequency remained unchanged in the cultured samples, showing a genetic structure change in cultured populations. The genetic distances between wild and cultured populations were 0.0421 and 0.0809. With primer combination Ga5-800-E5, we detected a region in the electrophoretic pattern in which the number of amplified loci apparently decreased in cultured populations. These results establish a good scientific basis for developing molecular markers that can help in distinguishing wild from cultured populations.

Keywords

grass carpCtenopharyngodon idellagenetic structuretarget region amplified polymorphism
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 4 , Issue 1 , April 2007 , pp. 27 - 32
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

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Footnotes

First published in Journal of Agricultural Biotechnology 2006, 14(4): 517–521

References

Bishop, JA and Cook, LM (1975) Moths, Melanism and Clear Air. Scientific American 232: 90–99.CrossRefGoogle Scholar
Hu, J and Vick, BA (2003) Target region amplification polymorphism: a novel marker technique for plant genotyping. Plant Molecular Biology Reporter 21: 289–294.CrossRefGoogle Scholar
Jaccard, P (1908) Nouvelles recherches sur la distribution florale. Bulletin De La Société Vaudoise des Sciences Naturelles 44: 223–270.Google Scholar
Li, G and Quiros, CF (2001) Sequence related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theoretical and Applied Genetics 103: 455–461.CrossRefGoogle Scholar
Li, SF (2001) The Research on Biology Diversity and Protection of Important Fishes in Yangtze River. Shanghai: Science and Technology Press (in Chinese).Google Scholar
Li, SF and Lv, GQ (1998) Diversity of mitochondrial DNA in the populations of silver carp, bighead carp, grass carp and black carp in the middle and lower reaches of the Yangtze River. Acta Zoology Sinica 44(1): 82–93 (in Chinese with English abstract).Google Scholar
Liao, XL, Yu, XM and Tan, DQ (2005) Microsatellite DNA analysis of genetic diversity of grass carp in Yangtze River System. Acta Hydrobiologica Sinica 29(2): 113–118 (in Chinese with English abstract).Google Scholar
Liao, YM, Liu, JL and Tang, XL (1994) Analysis on the degeneration of the four major Chinese carps and advising on the protection of the germplasm resources. Fisheries Science and Technology Information 21(2): 62–63 (in Chinese with English abstract).Google Scholar
Liu, SP, Qiu, SL, Chen, DQ and Huang, MG (1997) Protection and rational utilization of the germplasm resources of the four major Chinese carps in the Yangtze River System. Resources and Environment in the Yangtze Valley 6(2): 127–131 (in Chinese with English abstract).Google Scholar
Nei, M and Li, WH (1979) Mathematical modeling for studying genetic variation in terms of restriction endonuclease. Proceedings of the National Academy of Sciences of the USA 76: 5268–5273.Google Scholar
Wu, LZ and Wang, ZX (1997) Biochemical genetic structure and variation in a natural population of sliver carp from the middle reaches of the Yangtze River. Acta Hydrobiologica Sinica 21(2): 157–161 (in Chinese with English abstract).Google Scholar
Xu, SS, Hu, JG and Faris, JD (2003) Molecular characterization of the Langdon Durum–Triticum dicoccoides chromosome substitution lines using target region amplification polymorphism (TRAP) markers. Wheat Genetics International Symposium Proceedings. Instituto Sperimentale per la Cerealicoltura, Rome, Italy 1: 91–94.Google Scholar
Xue, GX, Liu, J and Liu, J (1998) RAPD Analysis of grass carp population in three river waters. Journal of Fishery Sciences of China 5(1): 1–5 (in Chinese with English abstract).Google Scholar
Yang, XM and Li, SF (1996) Growth differences and biochemical genetic changes between wild stock and hatchery populations of silver carp and grass carp from Yangtze River. Journal of Fishery Sciences of China 3(4): 1–10 (in Chinese with English abstract).Google Scholar
Zhang, DC, Yu, LN and Fang, YL (2004) Genetic diversity analysis on wild and cultured population of grass carp. Freshwater Fisheries 34: 5–7 (in Chinese with English abstract).Google Scholar
Zhang, SM, Deng, H and Wang, DQ (2001) Population structure and genetic biodiversity of silver carp and grass carp from populations of Yangtze River systems revealed by RAPD. Acta Hydrobiologica Sinica 25(4): 324–330 (in Chinese with English abstract).Google Scholar