Hostname: page-component-586b7cd67f-g8jcs Total loading time: 0 Render date: 2024-11-23T05:49:40.636Z Has data issue: false hasContentIssue false
  • English
  • Français

Establishment of a highly efficient regeneration system for in vitro culture of young wheat spikes

Published online by Cambridge University Press:  12 February 2007

Zhao Lin-Shu ,
Liu Lu-Xiang ,
Wang Jing ,
Zheng Qi-Cheng ,
Guo Hui-Jun ,
Zhao Shi-Rong  and
Chen Wen-Hua
Zhao Lin-Shu
Affiliation:
Key Laboratory of Agricultural Nuclear Technology and Space Breeding, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Liu Lu-Xiang*
Affiliation:
Key Laboratory of Agricultural Nuclear Technology and Space Breeding, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Wang Jing
Affiliation:
Key Laboratory of Agricultural Nuclear Technology and Space Breeding, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Zheng Qi-Cheng
Affiliation:
Key Laboratory of Agricultural Nuclear Technology and Space Breeding, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Guo Hui-Jun
Affiliation:
Key Laboratory of Agricultural Nuclear Technology and Space Breeding, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Zhao Shi-Rong
Affiliation:
Key Laboratory of Agricultural Nuclear Technology and Space Breeding, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Chen Wen-Hua
Affiliation:
Key Laboratory of Agricultural Nuclear Technology and Space Breeding, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
*
*Corresponding author. Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

This study used three winter wheat (Triticum aestivum L.) genotypes (H6756, H311 and SP8581) to compare the effects of sampling time, callus induction media, differentiation media and rooting media on in vitro culture of young spikes in wheat. In all these three genotypes, the frequencies of green plantlet differentiation were high when their young spikes were cultured between the stages of protective glume primordium formation and pistil and stamen primordium formation, but low at other stages. The optimum medium for callus induction was Murashige and Skoog (MS) medium+2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D). The optimum green plantlet differentiation medium was MS medium. Some abnormal plantlets regenerated from calli. When these plantlets were transferred to another differentiation medium [MS+1.0 mg/l 1-naphthaleneacetic acid (NAA)+0.2 mg/l 6-benzylaminopurine (6-BA)], shoot formation and elongation were induced. This allowed 90.91% of them to develop into normal green plantlets. The optimum rooting medium was 1/2MS+0.2 mg/l 3-Indolylacetonitrile (IAA)+80 g/l sucrose. An efficient regeneration system for young spike culture of wheat was set up based on such methods. Using this wheat-regeneration system, young spikes and immature embryos of 17 genotypes of wheat were in vitro cultured to study and compare the callus induction frequencies and green plantlet differentiation frequencies. The results of two successive years showed that in 15 out of the 17 genotypes (88.24%) the green plantlet differentiation frequencies were higher than those of immature embryos by 6.2–65.1%. These results showed that the regeneration system established in this trial for young spike culture of wheat was effective.

Keywords

efficient regeneration systemin vitro culturewheatyoung spike
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 3 , Issue 2 , August 2006 , pp. 147 - 154
Copyright
Copyright © China Agricultural University and Cambridge University Press 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Altpeter, F, Vasil, V, Srivastava, V, Stoger, E and Vasil, I (1996) Accelerated production of transgenic wheat (Triticums aestivum L.) plants. Plant Cell Reports 16: 12–17.CrossRefGoogle Scholar
Eapen, S and Rao, PS (1985) Plant regeneration from immature inflorescence callus cultures of wheat-rye and triticale. Euphytica 34: 153–159.CrossRefGoogle Scholar
Hou, BK, Yu, HM and Teng, SY (1997) Effects of low temperature on induction and differentiation of wheat callus. Plant Cell Tissue and Organ Culture 49: 35–38.CrossRefGoogle Scholar
Lang, ML, Chen, RM, Zhao, JJ, Wen, SM and Yang, XJ (2001) Investigation on several factors influencing the cultural effects of wheat young spikes. Plant Physiology Communications 37(5): 393–395 (in Chinese).Google Scholar
Liu, LX, Zheng, QC, Zhao, LS, et al. (2001) Study on receptor system for wheat genetic transformation without genotype dependence. Acta Agriculturae Nucleatae Sinica 15(5): 308–310 (in Chinese with English abstract).Google Scholar
Maddock, SE, Lancaster, VA, Risott, R and Franklin, J (1983) Plant regeneration from cultured immature embryos and inflorescences of 25 cultivars of wheat. Journal of Experimental Botany 34(144): 915–926.CrossRefGoogle Scholar
Marcinska, I, Biesaga, KJ, Dubert, F and Kozdoj, J (1999) Effect of length and developmental stage of spike on the induction and differentiation efficiency of callus tissue in winter wheat. Evidence for generative development of regenerated plants. Acta Physiologiae Plantarum 21(4): 355–363.CrossRefGoogle Scholar
Murashige, T and Skoog, F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473–497.CrossRefGoogle Scholar
Quan, JL, Liu, ZQ, Chen, YF, Han, DJ, Li, CL and Ren, HL (1999) Studies on the effect of sucrose and plant hormone on the formation and growth of immature inflorescences somaclone in wheat. Acta Botanica Boreali-Occidentalia Sinica 19(6): 87–91 (in Chinese with English abstract).Google Scholar
Rasco-Gaunt, S, Riley, A, Barcelo, P and Lazzeri, PA (1999) Analysis of particle bombardment parameters to optimize DNA delivery into wheat tissues. Plant Cell Reports 19(2): 118–127.CrossRefGoogle Scholar
Sharma, VK, Rao, A, Varshney, A and Kothari, SL (1995) Comparison of developmental stages of inflorescence for high frequency plant regeneration in Triticum aestivum L. and T. durum Desf. Plant Cell Reports 15(3): 227–231.CrossRefGoogle ScholarPubMed
Shu, LH (1982) The response of young spike of wheat at distinct development stages to in vitro culture. Chinese Science Bulletin 27(14): 882–884 (in Chinese).Google Scholar
Skoog, F and Miller, CO (1957) Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symposium of the Society for Experimental Biology 11: 118–131.Google ScholarPubMed
Viertel, K and Hess, D (1996) Shoot tips of wheat as an alternative source for regenerable embryogenic callus cultures. Plant Cell Tissue and Organ Culture 44: 183–188.CrossRefGoogle Scholar
Wang, P, Pei, CJ and Chen, YR (1990) The response of different inflorescence sections of winter wheat at distinct development stages to in vitro culture. Acta Agriculturae Boreali-Sinica 5(1): 28–32 (in Chinese with English abstract).Google Scholar
Wu, BH, Ren, ZL, Liu, DC and Huang, LX (1997) Variability of callus induction, growth and plant regeneration from immature inflorescence among wheat varieties. In: Huang, G and Zheng, Y-L (editors) Proceedings of Fourth National Youth Symposium on Crop Genetics and Breeding. Chengdu: Science and Technology Press, pp. 281–289 (in Chinese with English abstract).Google Scholar
Xiao, XG, Zhang, AM and Nie, XL (2000) Wheat genetic transformation: advances and prospects. Journal of Agricultural Biotechnology 8(2): 111–116 (in Chinese with English abstract).Google Scholar
Yu, SL (1980) Crop Cultivation. Beijing: Agriculture Press (in Chinese).Google Scholar
Zhao, LS (2005) Optimization of regeneration system of tissue culture and transformation of DREB1A gene in wheat. Masters thesis, Chinese Academy of Agricultural Sciences.Google Scholar
Zheng, QC, Zhu, YL and Chen, WH (1989) Plant regeneration from growing tips cultures of wheats and its variation. Acta Agriculturae Nucleatae Sinica 3(3): 129–136 (in Chinese with English abstract).Google Scholar