Hostname: page-component-586b7cd67f-rdxmf Total loading time: 0 Render date: 2024-11-26T19:08:39.785Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of donor sex and different cell treatments on development of yak–bovine interspecies nuclear transfer embryos

Published online by Cambridge University Press:  27 June 2008

Liu Ying ,
Zhu Shi-En ,
Li Rong ,
Wang Li-Li ,
Wang Hai-Ping ,
Ding Fang-Rong ,
Li Jing ,
Li Song  and
Dai Yun-Ping
Liu Ying
Affiliation:
Animal Sci-tech College, China Agricultural University, Beijing 100094, China Geneprint Biotechnology Co. Ltd, Beijing 100094, China
Zhu Shi-En
Affiliation:
Animal Sci-tech College, China Agricultural University, Beijing 100094, China
Li Rong
Affiliation:
Geneprint Biotechnology Co. Ltd, Beijing 100094, China
Wang Li-Li
Affiliation:
State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100094, China
Wang Hai-Ping
Affiliation:
Geneprint Biotechnology Co. Ltd, Beijing 100094, China
Ding Fang-Rong
Affiliation:
Geneprint Biotechnology Co. Ltd, Beijing 100094, China
Li Jing
Affiliation:
Geneprint Biotechnology Co. Ltd, Beijing 100094, China
Li Song
Affiliation:
Geneprint Biotechnology Co. Ltd, Beijing 100094, China
Dai Yun-Ping*
Affiliation:
State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100094, China
*
*Corresponding author. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The purpose of this study was to evaluate the effects of donor sex, treatments of cell cycle synchronization and donor nuclei obtained from fresh or frozen–thawed conditions on developmental competence of yak–bovine interspecies nuclear transfer embryos. Bovine (Bos taurus) oocytes were used as recipients and yak (Bos grunniens) ear fibroblast cells were used as donors. Results indicated that the development rate of male blastocysts was higher than that of female (56.6% versus 39.5%, P<0.05), whereas cleavage and total cell number showed no difference between the two groups. No significant difference was observed in the development and quality of blastocysts with donor cells treated by serum starvation or contact inhibition, and there was no significant difference in embryo development with fresh or frozen–thawed donor cells, whereas the cleavage rate in the group of frozen–thawed cells was significantly lower than that of the fresh cell group (54.5% versus 78.2%, P<0.05). The results demonstrated that donor sex could impact the developmental competence of yak–bovine interspecies nuclear transfer embryos, whereas different treatments of cell cycle synchronization and freezing had little influence.

Keywords

yakinterspecies nuclear transferdonor cellembryo development
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 1 , April 2008 , pp. 55 - 60
Copyright
Copyright © China Agricultural University 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

First published in Journal of Agricultural Biotechnology 2008, 16(1): 61–65

References

Cibelli, JB, Stice, SL, Golueke, PJ, et al. (1998) Cloned transgenic calves produced from nonquiescent fetal fibroblasts. Science 280(5367): 1256–1258.CrossRefGoogle ScholarPubMed
Dominko, T, Mitalipova, M, Haley, B, et al. (1999) Bovine oocyte cytoplasm supports development of embryos produced by nuclear transfer of somatic cell nuclei from various mammalian species. Biology of Reproduction 60(6): 1496–1502.CrossRefGoogle ScholarPubMed
Gómez, MC, Jenkins, JA, Giraldo, A, et al. (2003) Nuclear transfer of synchronized African wild cat somatic cells into enucleated domestic cat oocytes. Biology of Reproduction 69(3): 1032–1041.CrossRefGoogle ScholarPubMed
Guo, JT, An, ZX, Li, Y, et al. (2002) Cloned goats (Capra hircus) from adult ear cells. Science in China (Series C) 45: 260–267.CrossRefGoogle ScholarPubMed
Hashem, A, Bhandari, DP and Kang, SK (2007) Cell cycle analysis and interspecies nuclear transfer of in vitro cultured skin fibroblasts of the Siberian tiger (Panthera tigris altaica). Molecular Reproduction and Development 74(4): 404–411.CrossRefGoogle ScholarPubMed
Hayes, O, Ramos, B, Rodríguez, LL, Aguilar, A, Badía, T and Castro, FO (2005) Cell confluency is as efficient as serum starvation for inducing arrest in the G0/G1 phase of the cell cycle in granulosa and fibroblast cells of cattle. Animal Reproduction Science 87(3–4): 181–192.CrossRefGoogle ScholarPubMed
Kues, WA, Anger, M, Carnwath, JW, Paul, D, Motlik, J and Niemann, H (2000) Cell cycle synchronization of porcine fetal fibroblasts: effects of serum deprivation and reversible cell cycle inhibitors. Biology of Reproduction 62(2): 412–419.CrossRefGoogle ScholarPubMed
Lanza, RP, Cibelli, JB, Diaz, F, et al. (2000) Cloning of an endangered species (Bos gaurus) using interspecies nuclear transfer. Cloning 2(2): 79–90.CrossRefGoogle ScholarPubMed
Li, YX, Dai, YP, Du, WH, et al. (2007) In vitro development of yak (Bos grunniens) embryos generated by interspecies nuclear transfer. Animal Reproduction Science 101(1–2): 45–59.CrossRefGoogle ScholarPubMed
Loi, P, Ptak, G, Barboni, B, Fulka, J Jr, Cappai, P and Clinton, M (2001) Genetic rescue of an endangered mammal by cross-species nuclear transfer using post-mortem somatic cells. Nature Biotechnology 19(10): 962–964.CrossRefGoogle ScholarPubMed
Sansinena, MJ, Hylan, D, Hebert, K, Denniston, RS and Godke, RA (2005) Banteng (Bos javanicus) embryos and pregnancies produced by interspecies nuclear transfer. Theriogenology 63(4):1081–1091.CrossRefGoogle ScholarPubMed
Wilmut, I, Schnieke, AE, McWhir, J, Kind, AJ and Campbell, KH (1997) Viable offspring derived from fetal and adult mammalian cells. Nature 385(6619): 810–813.CrossRefGoogle ScholarPubMed
Xue, F, Tian, XC, Du, F, et al. (2002) Aberrant patterns of X chromosome inactivation in bovine clones. Nature Genetics 31(2): 216–222.CrossRefGoogle ScholarPubMed