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Effect of some factors on the fusion rate of bovine–rabbit interspecies reconstructed eggs

Published online by Cambridge University Press:  12 February 2007

Zhang Xiao-Rong ,
Liu Ya ,
Zhang Yun-Hai ,
Chen Da-Yuan ,
Lian Li ,
Li Jin-Song ,
Jin Ren-Tao ,
Zhang Zhi-Guo ,
Zhang Mei-Ling  and
Wang Cun-Li
Zhang Xiao-Rong*
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China Animal Science and Technology College, Anhui Agricultural University, Hefei 230036, China
Liu Ya
Affiliation:
Animal Science and Technology College, Anhui Agricultural University, Hefei 230036, China
Zhang Yun-Hai
Affiliation:
College of Biotechnology, China Agricultural University, Beijing 100094, China
Chen Da-Yuan
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
Lian Li
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
Li Jin-Song
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
Jin Ren-Tao
Affiliation:
Animal Science and Technology College, Anhui Agricultural University, Hefei 230036, China
Zhang Zhi-Guo
Affiliation:
Animal Science and Technology College, Anhui Agricultural University, Hefei 230036, China
Zhang Mei-Ling
Affiliation:
Animal Science and Technology College, Anhui Agricultural University, Hefei 230036, China
Wang Cun-Li
Affiliation:
Animal Science and Technology College, Anhui Agricultural University, Hefei 230036, China
*
*Corresponding author: E-mail: [email protected] or [email protected]
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Abstract

Tests were conducted to highlight the effects of donor cell treatment (bovine G0- and non-G0 phase somatic cells refrigerated for 1–5 or 12–14 days at 4°C), fusion solution (with or without cytochalasin B) and age of the rabbit (young, 3–5-months old; or multiparous, 10–12 months old) as oocyte donor on the fusion and mortality rates of bovine–rabbit interspecies reconstructed eggs. Results showed that there were no significant differences (P>0.05) in fusion and mortality rates of eggs reconstructed with G0 cells or non-G0 cells as nuclear donor somatic cells. The same was also true when nuclear donor somatic cells (G0 cells or non-G0 cells) were treated at low temperature (4°C) for 1–5 or 12–14 days. The fusion rate of reconstructed eggs did not change significantly (P>0.05) when adding cytochalasin B into the fusion solution, but the mortality rate increased significantly (P<0.05). No significant effect (P>0.05) was found in fusion rate of the eggs reconstructed with young rabbits' and multiparous rabbits' oocytes as recipient, but the mortality rate decreased significantly (P<0.01) for young rabbit oocytes compared with multiparous rabbit ones.

Keywords

nuclear donor cellreconstructed eggcytochalasin Bfusion rate
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 1 , Issue 3 , December 2004 , pp. 135 - 138
Copyright
Copyright © China Agricultural University and Cambridge University Press 2004

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