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Construction and utilization of plant expression vectors with PMI gene as selection marker to recover transgenic plants of Citrus sinensis L. Osbeck

Published online by Cambridge University Press:  24 April 2009

Zeng Li-Hui ,
Xu Hai-Feng ,
Wang Hui-Quang ,
Wu Shao-Hua  and
Zhu Yi-Xuan
Zeng Li-Hui*
Affiliation:
College of Horticulture, Fujian Agricultural and Forestry University, Fuzhou 350002, China
Xu Hai-Feng
Affiliation:
College of Horticulture, Fujian Agricultural and Forestry University, Fuzhou 350002, China
Wang Hui-Quang
Affiliation:
College of Horticulture, Fujian Agricultural and Forestry University, Fuzhou 350002, China
Wu Shao-Hua
Affiliation:
College of Horticulture, Fujian Agricultural and Forestry University, Fuzhou 350002, China
Zhu Yi-Xuan
Affiliation:
College of Horticulture, Fujian Agricultural and Forestry University, Fuzhou 350002, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

The plant expression vectors pCAMBIA1301PMI and pBIPMI were constructed by substituting the Escherichia coli phosphomannose-isomerase (PMI) gene for the hpt gene of pCAMBIA1301 and gus gene of pBI121, respectively. Epicotyl explants of the Xuegan sweet orange (Citrus sinensis L. Osbeck) were inoculated with Agrobacterium tumefaciens EHA105- pCAMBIA1301PMI and EHA105-pBIPMI and subsequently selected in a medium supplemented with a combination of 25 g/l mannose and 5 g/l sucrose as the carbon source. The transformation efficiency rate was 27.7% when transformed by pCAMBIA1301PMI and 12.7% by pBIPMI. Genetic transformation was confirmed by chlorophenol red assay and polymerase chain reaction (PCR). A new method for obtaining transgenic Xuegan sweet orange plants was developed using the PMI/mannose selection system.

Keywords

phosphomannose-isomerase (PMI) genemannoseXuegan sweet orangegenetic transformation
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 6 , Issue 1 , April 2009 , pp. 11 - 18
Copyright
Copyright © China Agricultural University 2009

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References

Boscariol, RL, Almeida, WAB, Derbyshire, MTVC, Mourão Filho, FAA and Mendes, BMJ (2003) The use of the PMI/mannose selection system to recover transgenic sweet orange plants (Citrus sinensis L. Osbeck). Plant Cell Reports 22: 122–128.CrossRefGoogle ScholarPubMed
Degenhardt, J, Poppe, A, Montag, J and Szankowski, I (2006) The use of the phosphomannose-isomerase/mannose selection system to recover transgenic apple plants. Plant Cell Reports 25: 1149–1156.CrossRefGoogle ScholarPubMed
Peng, SQ and Chen, SC (2005) A novel selection system for tomato transformation by mannose. Journal of Agricultural Biotechnology 13(2): 141–144.Google Scholar
Weisser, P, Kramer, R and Sprenger, GA (1996) Expression of the Escherichia coli pmi gene, encoding phosphomannose-isomerase in Zymomonas mobilis, leads to utilization of mannose as a novel growth substrate, which can be used as a selective marker. Applied and Environmental Microbiology 6(11): 4155–4161.CrossRefGoogle Scholar
Xu, H, Luan, A, Zeng, L and Wu, S (2007) High efficiency induction of adventitious roots and in vitro regeneration system of sweet orange ‘Xuegan’. Acta Agriculturae Universitatis Jiangxiensis 29(1): 148–151.Google Scholar
Yue, J, Meng, Z, Zhang, L, Han, S, Ling, Q and Wang, W (2005) Cotton transformation with mannose as selective agent. Cotton Science 17(1): 3–7.Google Scholar
Yu, CH, Huang, S, Chen, CX, Deng, ZA, Ling, P and Gmitter, FGJ (2002) Factors affecting Agrobacterium-mediated transformation and regeneration of sweet orange and citrange. Plant Cell, Tissue and Organ Culture 71: 147–155.CrossRefGoogle Scholar
Zeng, LH (2001) Agrobacterium tumefaciens-mediated genetic transformation of litchi (Litchi chinensis Sonn.) with LEAFY gene from Arabidopsis thaliana. Fuzhou: Fujian Agricultural University.Google Scholar