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Association of IL-4 (intron 3) and IL-10 (-1082) gene polymorphisms with risk of mitral valve disease in children with rheumatic heart disease

Published online by Cambridge University Press:  28 October 2015

Sherif M. Yousry ,
Yasser Sedky  and
Alaa Sobieh
Sherif M. Yousry*
Affiliation:
Department of Clinical Pathology, Cairo University, Giza, Egypt
Yasser Sedky
Affiliation:
Department of Pediatrics, Cairo University, Giza, Egypt
Alaa Sobieh
Affiliation:
Department of Pediatrics, Cairo University, Giza, Egypt
*
Correspondence to: S. M. Yousry, Department of Clinical Pathology, Cairo University, 132 Al Bahr Al Azam Street, Giza 11221, Egypt. Tel: (+2)0235736727; Fax: (+2)0235700977; E-mail: [email protected]
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Abstract

Aim

Rheumatic heart disease is an inflammatory disease of cardiac tissue. The underlying pathogenic mechanisms highlight a complex interplay of immunological, genetic, and environmental factors. The aim of the present study was to investigate whether IL-4 (intron 3) and IL-10 (-1082) gene polymorphisms could be associated with susceptibility and/or severity of rheumatic heart disease among patients from the Egyptian population.

Materials and methods

A cohort of 140 Egyptian children with rheumatic heart disease and 100 healthy controls were enrolled in this case–control study. Genotyping for IL-4 (intron 3) and IL-10 (-1082) gene polymorphisms was carried out for all patients using a polymerase chain reaction-based analysis.

Results

No significant difference in the distribution of genotypes and allelic frequencies between rheumatic heart disease cases and controls for IL-4 (intron 3) (p=0.17; OR 1.07, 95% CI 0.82–3.74) and IL-10 (-1082) (p=0.49; OR 1.03, 95% CI 0.65–2.71) gene polymorphisms was observed. Further categorisation of patients into mitral valve disease and combined valve disease subgroups showed that cases with mitral valve disease have significantly higher frequency of the RP2 allele of IL-4 (intron 3) (p=0.03; OR 2.98, 95% CI 1.93–6.15) and the G allele of IL-10 (-1082) (p=0.04; OR 2.14, 95% CI 1.62–4.95) when compared with controls.

Discussion

Our study shows that IL-4 (intron 3) and IL-10 (-1082) gene polymorphisms are not significantly associated with susceptibility to rheumatic heart disease, but they might play a role in the pathogenesis of patients with mitral valve disease.

Keywords

Rheumatic heart diseaseIL-4IL-10polymerase chain reactionmitral valve disease
Type
Original Articles
Information
Cardiology in the Young , Volume 26 , Issue 7 , October 2016 , pp. 1290 - 1296
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Copyright
© Cambridge University Press 2015 

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