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Sarcoplasmic Reticulum Calcium Release Channels in Ventricles of Older Adult Hamsters*

Published online by Cambridge University Press:  31 March 2010

Peter A. Nicholl  and
Susan E. Howlett
Peter A. Nicholl
Affiliation:
Department of Pharmacology, Dalhousie University
Susan E. Howlett*
Affiliation:
Department of Pharmacology, Dalhousie University
*
Requests for offprints should be sent to: / Les demandes de tirés-à-part doivent être addressées à : S.E. Howlett, Ph.D., Department of Pharmacology, 5850 College St., Dalhousie University, Halifax, NS B3H 1X5. ([email protected])
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Abstract

Whether the density of sarcoplasmic reticulum (SR) calcium release channels / ryanodine receptors in the heart declines with age is not clear. We investigated age-related changes in the density of «3H»-ryanodine receptors in crude ventricular homogenates, which contained all ligand binding sites in heart and in isolated junctional SR membranes. Experiments utilized young (120 days) and older adult (300 days) hamsters. «3H»-ryanodine binding site density did not change with age in crude homogenate preparations, although total heart protein concentration increased significantly with age. In contrast, the density of «3H»-ryanodine binding sites decreased markedly in heavy SR membranes purified from older hearts. These results show that demonstration of age-related changes in cardiac ryanodine receptor density depends upon the preparation used. Furthermore, the increase in total ventricular protein with age suggests that normalization of data by membrane protein should be used with caution in studies of aging heart.

Résumé

Il n/est pas certain que la densité des canaux calciques / récepteurs à la ryanodine du réticulum sarcoplasmique (RS) dans le muscle cardiaque diminue en fonction de l/âge. Nous avons analysé les changements liés à l/âge dans la densité des récepteurs à la «3H»-ryanodine dans des broyats ventriculaires grossiers contenant tous les sites de liaison des ligands dans le muscle cardiaque, ainsi que dans les membranes jonctionnelles isolées du RS. Des expériences ont été réalisées sur de jeunes hamsters (de 120 jours) et sur des hamsters adultes (de 300 jours). La densité des sites de liaison de la «3H»-ryanodine ne changeait pas dans les préparations de broyat grossier, mais la concentration totale des protéines cardiaques augmentait de façon significative en fonction de l/âge. Par contre, la densité des sites de liaison de la «3H»-ryanodine diminuait sensiblement dans les membranes lourdes purifiées du réticulum sarcoplasmique provenant de coeurs plus âgés. Ces résultats montrent que les démonstrations des changements liés à l/âge dans la densité des récepteurs à la ryanodine dans le muscle cardiaque dépendent du type de préparation utilisée. De plus, l/augmentation des protéines ventriculaires totales en fonction de l/âge suggère qu/il convient d/être prudent lorsqu/on procède à une normalisation des données en fonction des protéines de la membrane dans les études sur le vieillissement du muscle cardiaque.

Keywords

agingsarcoplasmic reticulumcalcium release channelexcitation-contraction couplingryanodine receptorvieillissementréticulum sarcoplasmiquecanaux calciquescouplage excitation/contractionrécepteurs à la ryanodine
Type
Articles
Information
Canadian Journal on Aging / La Revue canadienne du vieillissement , Volume 25 , Issue 1 , Spring/Printemps 2006 , pp. 107 - 113
Copyright
Copyright © Canadian Association on Gerontology 2006

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Footnotes

*

The authors would like to thank H. Chan for excellent technical assistance. This study was supported by a grant from the Canadian Institutes of Health Research.

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