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A Simple and Reproducible Experimental in Vivo Glioma Model

Published online by Cambridge University Press:  18 September 2015

R.N. Auer ,
R.F. Del Maestro  and
R. Anderson
R.N. Auer
Affiliation:
Departments of Pathology, (Neuropathology), Brain Research Laboratory, Clinical Neurological Sciences, and Departments of Microbiology and immunology, University of Western Ontario London, Canada
R.F. Del Maestro*
Affiliation:
Departments of Pathology, (Neuropathology), Brain Research Laboratory, Clinical Neurological Sciences, and Departments of Microbiology and immunology, University of Western Ontario London, Canada
R. Anderson
Affiliation:
Departments of Pathology, (Neuropathology), Brain Research Laboratory, Clinical Neurological Sciences, and Departments of Microbiology and immunology, University of Western Ontario London, Canada
*
Brain Research Laboratory, Dept. of Clinical Neurological Sciences, Victoria Hospital, London, Ontario N6A 4G5
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A simple and reproducible injection technique has been developed for inducing an in vivo experimental astrocytoma model in rats. Newborn rats were injected with a suspension of astrocytoma C-6 cells in doses ranging from 5 to 1 X 105 cells. The optimum dose of tumor cells was found to be between 104 and 105 cells, with a consistent 100% take rate seen above 1 X 104 injected cells. The injection of less than 104 cells resulted in a decreased ability to induce tumors, and a prolonged survival. The pathology was consistent with that of a glioblastoma multiforme. The tumor showed a diffuse infiltrating border, necrosis, and pseudopalisading. Light microscopy revealed undifferentiated tumor cells while electron microscopy demonstrated rough endoplasmic reticulum, Golgi complexes, mitochondria, and the occasional cilium and centriole. The nature of the astrocytoma C-6 cell line, and the advantages, disadvantages and possible uses of the model are discussed.

Type
Research Article
Information
Canadian Journal of Neurological Sciences , Volume 8 , Issue 4 , November 1981 , pp. 325 - 331
Copyright
Copyright © Canadian Neurological Sciences Federation 1981

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