Background: Radiofrequency rhizotomy is an efficacious technique for treatment of trigeminal neuralgia that is classically performed with the patient awake. Previous studies have investigated methods for both anatomic and neurophysiologic optimization for nerve targeting. Methods: We performed a retrospective review of prospectively collected data on patients undergoing radiofrequency rhizotomy under a general anesthetic. Electrodes are placed in the temporalis, masseter and one of mylohyoid or anterior belly of digastric muscles. We then localize of the correct subdivision of the trigeminal nerve. The division of the trigeminal nerve with pain shows a muscle response, which is not present in normal subdivisions. Results: A total of 23 radiofrequency rhizotomies were performed under general anesthetic. Abnormal conduction reflexes were present in all cases, and dissipated after lesioning. Pre-operative BNI pain scores were 4.1 ± 0.3, which dropped to 1.8 ± 1.9 post-op (p=0.003). Number of pain medications (2.9 ± 0.6 v. 1.3 ± 1.3, p=0.007) and number of patients with opioid usage (78.7% v. 21.7%, p=0.007) both decreased after rhizotomy. Conclusions: Using a novel conduction pathway, we have successfully been able to monitor radiofrequency rhizotomy in patients operated on under a general anesthetic, with promising preliminary results. Further work is needed to better evaluate this intervention.