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Ouabain Induced Seizures: Site of Production and Response to Anticonvulsants

Published online by Cambridge University Press:  18 September 2015

Duncan L.W. Davidson ,
Yasuo Tsukada  and
André Barbeau
Yasuo Tsukada
Affiliation:
Department of Neurobiology, Clinical Research Institute of Montreal
André Barbeau*
Affiliation:
Department of Neurobiology, Clinical Research Institute of Montreal
*
Clinical Research Institute of Montreal, 110 Pine Avenue West, Montreal, Quebec, Canada.
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Ouabain, an inhibitor of Na+-K+-ATP'ase, has been administered intraventricularly to rats to study the effect of impairment of membrane transport mechanisms on the genesis of seizures. Running and leaping seizures occur rapidly after injection oj ouabain in a low volume (10μl) when the maximal uptake of ouabain (39.8%) is in the hippocampus. Generalized clonic-lonic seizures are induced by higher volume injections (50μl) associated with wider distribution of ouabain, including the cerebellum and brainstem.

Ouabain was injected into cerebral cortex, caudate nucleus, dorsal hippocampus, fastigeal nucleus, ventrolateral mesencephalic reticular formation and cerebellar cortex. The cerebellar injections produced both running and leaping and generalized clonic-lonic seizures. It is suggested that this results from decreased inhibitory effect of vermal and paravermal Purkinje cells on intra-cerebellar nuclei, which alters cerebellar influence on the reticular formation and the limbic system.

Diphenylhydantoin, phenobarbitone, phenacemide, carbamezepine and clonazepam but not ethosuximide are effective against generalized clonic-lonic seizures, suggesting that this is a model for “grand mat” but not “petit mal” seizure mechanisms. It is furthermore suggested that running and leaping are subcortical, probably limbic, seizures that are most relevant as a model for temporal lobe seizures.

Type
Research Article
Information
Canadian Journal of Neurological Sciences , Volume 5 , Issue 4 , November 1978 , pp. 405 - 411
Copyright
Copyright © Canadian Neurological Sciences Federation 1978

References

REFERENCES

Ajmone Marsan, C. (1972). Focal electrical stimulation. In: (Purpura, D.P., Penry, D., Woodbury, D.M. and Walter, R., eds). Experimental Models of Epilepsy. Raven Press, New York, pp. 147–162.Google Scholar
Alberici, M.Rodriguez De Lores Arnais, B. and De Robertis, E.Glutamic acid decarboxylase inhibition and ultrastructural changes by the convulsant drug allylglycine. Biochemical Pharmacology, 18, 137–143.CrossRefGoogle Scholar
Babb, T.H., Mitchell, A.G., and Crandall, P.H. (1974). Fastigiobulbar and dentatothalamic influence on hippo-campal cobalt epilepsy in the cat. Electroencephalography and Clincial Neurophysiology, 36, 144–154.Google Scholar
Baldy-Moulinier, M., Arias, L.P., and Passouant, P. (1973). Hippocampal epilepsy produced by ouabain. Europ. Neurol., 9, 333–348.CrossRefGoogle ScholarPubMed
Bennett, J.P., Logan, W.J. and Snyder, S.H. (1973). Aminoacids as central nervous transmitters: the influence of ions, amino acid analogues, and ontogeny on transport systems for L-glutamic and L-aspartic acids and glycine into central nervous synaptosomes of the rat. Journal of Ncurochemistry, 21, 1533–1550.CrossRefGoogle ScholarPubMed
Bergmann, F., Costin, A. and Gutman, J. (1963). A low threshold convulsive area in the rabbits mesencephalon. Electroencephalography and Clinical Neurophysiology, 15, 683–690.CrossRefGoogle ScholarPubMed
Bergmann, F., Costin, A., Chaimovitz, M., and Zerachia, A. (1970). Seizure activity evoked by implantation of ouabain and related drugs into cortical and subcortical regions of the rabbit brain. Neuropharmacology, 9, 441–449.CrossRefGoogle ScholarPubMed
Bogdanski, D.F., Tissari, A., and Brodie, B.B (1968). Role of Sodium, potassium, ouabain and reserpine in uptake, storage and metabolism of biogenic amines in synaptosomes. Life Sciences, 7, 419–428.CrossRefGoogle ScholarPubMed
Chen, R.C., Forster, F.M. (1973). Cursive epilepsy and gelastic epilepsy. Neurology, 23, 1019–1028.CrossRefGoogle ScholarPubMed
Cooke, P.M., Snider, R.S. (1955). Some cerebellar influences on electrically-induced cerebral seizures. Epilepsia, 4, 19–28.CrossRefGoogle ScholarPubMed
Cooper, I.S., Crighel, E., and Amin, I. (1973). Clinical and physiological effects of stimulation of the paleocerebellum in human. Journal of the American Geriatrics Society, 21, 40–43.CrossRefGoogle Scholar
Cooper, I.S., Amin, I., and Gilman, S. (1973). The effect of chronic cerebellar stimulation upon epilepsy in man. Transactions of the American Neurological Association, 98, 7–11.Google ScholarPubMed
Cornog, J., Gonotas, N.K., and Fierman, J.R. (1967). Effects of intracerebral injection of ouabain on the fine structure of rat cerebral cortex. American Journal of Pathology, 51, 573–590.Google ScholarPubMed
Dahl, J.L., and Hokin, L.E. (1974). The sodium-potassium adenosine triphosphatase. Annual Review of Biochemistry, 43, 327–356.CrossRefGoogle Scholar
Davidson, D.L.W., and Barbeau, A. (1975). Locomotor activity and seizures induced by picrotoxin in the fastigeal nucleus of the rat. Experimental Neurology, 49, 622–627.CrossRefGoogle ScholarPubMed
De Robertis, E., Rodriguez De Lores Arnais, G. and Alberici, M. (1969). Ultrastructural neurochemistry. In: (Jasper, H.H., Ward, A.A., and Pope, A., eds.). Basic Mechanisms of the Epilepsies. Little Brown, Boston, pp. 146–151.Google Scholar
Donaldson, J., St.-Pierre, T., Minnich, J.L., and Barbeau, A. (1971). Seizures in rats associated with divalent cation inhibition of Na+-K+-APT’ase. Canadian Journal of Biochemistry, 49, 1217–1224.CrossRefGoogle Scholar
Dow, R.S. (1965). Extrinsic regulatory mechanisms of seizure activity. Epilepsia, 6, 122–140.CrossRefGoogle ScholarPubMed
Glowinski, J., and Iversen, L.L. (1966). Regional studies of catecholamines in the rat brain. I- The disposition of 3(H) norepi-nephrine, 3(3) dompamine and 3(H) dopa in various regions of the brain. Journal of Neurochemistry, 13, 655–669.CrossRefGoogle Scholar
Halpern, L.M., and Julien, R.M. (1972). Augmentation of cerebellar Purkinje cells discharge rate after diphenylhydentoin. Epilepsia, 13, 377–395.CrossRefGoogle Scholar
Harper, J.W., Heath, R.G. (1973). Anatomic connections of the fastigeal nucleus to the rostral forebrain in the cat. Experimental Neurology, 39, 285–292.CrossRefGoogle Scholar
Horion, R.W.. and Meldrum, B.S. (1973). Seizures induced by allylglycine, 3 mcrcaptoproprionic acid and 4-deoxypyri-doxine in mice and photosensitive baboons, and different modes of inhibition of cerebral glutamic acid decarboxylase. British Journal of Pharmacology and Chemotherapy, 49, 52–63.Google Scholar
Hutton, T., Frost, J.D. and Foster, J. (1972). The influence of the cerebellum in cat penicillin epilepsy. Epilepsia, 13. 401–408. Iwata, M. and Snider, R.S. (1959).CrossRefGoogle ScholarPubMed
Iwata, M., and Snider, R.S. (1959). Cerebello-hippocampal influence on the electroencephalogram. Electroencephalography and Clinical Neurophysiology, 2, 439–446.CrossRefGoogle Scholar
Izumi, K.Donaldson, J.. Minnich, J.L. and Barbeau, A. (1973). Ouabain-induced seizures in rats: Suppressive effects of taurine and 7-aminobutyric acid. Canadian Journal of Physiology and Pharmacy, 51, 885–889.CrossRefGoogle Scholar
Julien, R.M., and Halpern, L.M. (1972). Effects of diphenylhydantoin and other antiepileptic drugs on epileptiform activity and Purkinje cell discharge rats. Epilepsia, 13, 387–400.CrossRefGoogle Scholar
Konig, J.R.F., and Klippel, R.A. (1963). The Rat Brain, Williams and Wilkins Company, Baltimore.Google Scholar
Lewin, E. (1970) Epileptogenic foci induced with ouabain. Electroencephalography and Clincial Neurophysiology, 29, 402–403.CrossRefGoogle ScholarPubMed
Myers, R.D. (1966). Injection of solutions into cerebral tissue: Relation between volume and diffusion. Phys. Behav., 1, 171–174.CrossRefGoogle Scholar
Pappius, H., and Elliott, K.A.C. (1954). Adenosine triphosphatase, electrolytes and oxygen uptake rate of human normal and epileptogenic cerebral cortex. Canadian Journal of Biochemistry and Physiology, 37. 484–490.CrossRefGoogle Scholar
Pedley, T.A., Zuckerman, E.C., and Glaser, G.H. (1969). Epileptogenic effects of localized ventricular perfusion of ouabain on dorsal hippocampus. Experimental Neurology, 25, 207–219.CrossRefGoogle ScholarPubMed
Pellegrino, L.J., and Cushman, A.J. (1967). A Stereotaxic Atlas of the Rat Brain, Appleton Century-Croft, New York.Google Scholar
Petsche, H., Rappelsberg, P., Frey, Z.S., and Suchatski, B.U. (1973). The epileptogenic effect of ouabain (g-strophan-thin), it’s action on the EGG and cortical morphology. Epilepsia, 14, 243–260.CrossRefGoogle Scholar
Purpura, D.P.. Penry, J.K., Tower, D., Woodbury, D.M. and Walters, R. (Eds.) (1972). Experimental Models of Epilepsy, Raven Press, New York, pp. 1–615.Google Scholar
Racine, R.J. (1972). Modification of seizure activity by electrical stimulation: I. After-discharge threshold. Electroencephalo-graphy and Clincial Neurophysiology. 32, 269–279.CrossRefGoogle ScholarPubMed
Rapport, R.L., Harris, A.B., Friez, P.N., Ojemann, G.A. (1975). Human epileptic brain: N, K, ATP’ase activity and phenytoin concentrations. Archives of Neurology, 32, 549–554.CrossRefGoogle Scholar
Reis, D.J., Doba, N., and Nathan, M.A. (1973). Predatory attack, grooming, and consumatory behaviors evoked by electrical stimulation of cat cerebellar nuclei. Science, 182. 845–847.CrossRefGoogle Scholar
Skou, J.C. (1965). Enzymatic basis for active transport of Na+ and K+ across cell membrane. Physiological Reviews, 46, 596–615.CrossRefGoogle Scholar
Testa, G., and Gloor, P. (1974). Generalized penicillin epilepsy in the cat: Effect of midbrain cooling. Electroencephalography and Clinical Neurophysiology, 36, 517–524.CrossRefGoogle ScholarPubMed
Tower, D.B. (1965). Problems associated with studies of electrolyte metabolism in normal and epileptogenic cerebral cortex. Epilepsia. 6. 183–197.CrossRefGoogle ScholarPubMed
Tower, D.B. (1968). Ouabain and the distribution of calcium and magnesium in cerebral tissues in vitro. Experimental Brain Research, 6, 273–283.CrossRefGoogle ScholarPubMed
Tsukada, Y., Inoue, N., Donaldson, J., and Barbeau, A. (1974). Suppressive effects of various amino acids against .ouabain-induced seizures in rats. Canadian Journal of Neurological Sciences, 1, 214–221.CrossRefGoogle Scholar
White, T.D., and Keen, P. (1971). Effects of inhibitors of Na+-K+) - dependent adenosine triphosphatase on the uptake of norepine-phrine by synaptosomes. Molec. Pharm. 7, 40–45.Google Scholar
Udvarhelyi, G.B., and Walker, E.A. (1965). Dissemination of acute focal seizures in the monkey. Archives of Neurology, 12. 333–356.CrossRefGoogle ScholarPubMed