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Malondialdehyde, Glutathione Peroxidase, and Superoxide Dismutase in Cerebrospinal Fluid During Cerebral Vasospasm in Monkeys

Published online by Cambridge University Press:  18 September 2015

R. Loch Macdonald ,
Bryce K.A. Weir ,
Tim D. Runzer  and
Michael G.A. Grace
R. Loch Macdonald
Affiliation:
Division of Neurosurgery, University of Alberta, Edmonton
Bryce K.A. Weir*
Affiliation:
Division of Neurosurgery, University of Alberta, Edmonton
Tim D. Runzer
Affiliation:
Division of Neurosurgery, University of Alberta, Edmonton
Michael G.A. Grace
Affiliation:
Department of Surgery, University of Alberta, Edmonton
*
Department of Surgery, 2D. 102 Walter MacKenzie Health Sciences Center, University of Alberta, Edmonton, Alberta, Canada T6G 1V6
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Abstract:

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Cerebral vasospasm may result from lipid peroxidation induced by oxyhemoglobin in the subarachnoid space after subarachnoid hemorrhage. To test this theory, vasospasm was induced in monkeys by intrathecal injections of oxyhemoglobin or supernatant fluid from autologous blood incubated in vitro. Concentration of malondialdehyde (MDA), a product of lipid peroxidation, was elevated in cerebrospinal fluid (CSF) in association with vasospasm caused by oxyhemoglobin and supernatant fluid. Intrathecal injections of methemoglobin or bilirubin did not cause vasospasm or increased CSF MDA. Activity of glutathione peroxidase in CSF increased significantly after injection of oxyhemoglobin and methemoglobin. There were no significant changes in CSF superoxide dismutase activity although there was a trend towards higher activities in animals treated with oxyhemoglobin, methemoglobin, bilirubin, and supernatant fluid. These results show oxyhemoglobin-induced vasospasm is associated with MDA and lipid peroxidation in the subarachnoid space. Furthermore, detection of peroxidation products after injection of oxyhemoglobin in the absence of erythrocyte membranes indicates that oxyhemoglobin may directly damage cerebral arteries and brain by inducing lipid peroxidation in these structures. Depletion of free-radical scavenging enzymes in CSF did not seem necessary for development of vasospasm. In fact, there was a tendency for vasospasm to elevate enzyme activities, as if production of scavengers was induced by excess free radicals in the subarachnoid space.

Type
Articles
Information
Canadian Journal of Neurological Sciences , Volume 19 , Issue 3 , August 1992 , pp. 326 - 332
Copyright
Copyright © Canadian Neurological Sciences Federation 1992

References

1.Asano, T, Tanishima, T,. Sasaki, T, Sano, K.Possible participation of free radical reactions initiated by clot lysis in the pathogenesis of vasospasm after subarachnoid hemorrhage. In: Wilkins, RH, ed. Cerebral Arterial Spasm. Baltimore, Williams and Wilkins 1980; 190–201.Google Scholar
2.Steele, JA, Stockbridge, N, Maljkovic, G, Weir, B.Free radicals mediate actions of oxyhemoglobin on cerebrovascular smooth muscle cells. Circ Res 1991; 68: 416–423.CrossRefGoogle ScholarPubMed
3.Sasaki, T, Asano, T, Sano, K.Cerebral vasospasm and free radical reactions. Neurol Med Chir (Tokyo) 1980; 20: 145–153.CrossRefGoogle ScholarPubMed
4.Sasaki, T, Wakai, S, Asano, T, Watanabe, T, Kirino, T, et al. The effect of a lipid hydroperoxide of arachidonic acid on the canine basilar artery. J Neurosurg 1981; 54: 357–365.CrossRefGoogle ScholarPubMed
5.Macdonald, RL, Weir, BKA, Saito, K, Kanamaru, K, Findlay, JM, et al. Etiology of cerebral vasospasm in primates. J Neurosurg 1991; 75: 415–424.Google Scholar
6.Elliott, KAC, Jasper, HH.Physiological salt solutions for brain surgery. J Neurosurg 1949; 6: 140–152.CrossRefGoogle ScholarPubMed
7.Martin, W, Villani, GM, Jothianandan, D, Furchgott, RF.Blockade of endothelium-dependent and glyceryl trinitrate-induced relaxation of rabbit aorta by certain ferrous hemoproteins. J Pharmacol Exp Ther 1985; 233: 679–685.Google ScholarPubMed
8.Bull, AW, Marnett, LJ.Determination of malondialdehyde by ionpairing high-performance liquid chromatography. Analytical Biochem 1985; 149: 284–290.CrossRefGoogle ScholarPubMed
9.Marklund, SL.Direct assay with potassium superoxide. In: Greenwald, RA, ed. CRC Handbook of Methods for Oxygen Radical Research. Boca Raton, CRC Press Inc., 1985; 249–255.Google Scholar
10.Wendel, A.Glutathione peroxidase. In: Jakoby, WB, ed. Methods in Enzymology. New York, Academic Press Inc. 1981; 77: 325–333.Google Scholar
11.Gunzler, WA, Kremers, H, Flohe, L.An improved coupled test procedure for glutathione peroxidase (EC 1.11.1.9) in blood. Z Klin Chem Klin Biochem 1974; 12: 444–448.Google Scholar
12.Liszczak, TM, Varsos, VG, Black, PMcL, Kistler, JP, et al. Cerebral arterial constriction after experimental subarachnoid hemorrhage is associated with blood components within the arterial wall. J Neurosurg 1983; 58: 18–26.CrossRefGoogle ScholarPubMed
13.Fujii, S, Fujitsu, K.Experimental vasospasm in cultured arterial smooth-muscle cells. Part I: contractile and ultrastructural changes caused by oxyhemoglobin. J Neurosurg 1988; 69: 92–97.CrossRefGoogle ScholarPubMed
14.Okada, H, Endo, S, Kamiyama, K, Suzuki, J.Oxyhemoglobin-induced cerebral vasospasm and sequential changes of vascular ultrastructure. Neurol Med Chir (Tokyo) 1980; 20: 573–582.CrossRefGoogle ScholarPubMed
15.Halliwell, B, Gutteridge, JMC.Oxygen free radicals and iron in relation to biology and medicine: some problems and concepts. Arch Biochem Biophys 1986; 246: 501–514.CrossRefGoogle ScholarPubMed
16.Kanamaru, K, Weir, BKA, Findlay, JM, Grace, M, Macdonald, RL.A dosage study of the effect of the 21-aminosteroid U74006F on chronic cerebral vasospasm in a primate model. Neurosurgery 1990; 27: 29–38.CrossRefGoogle Scholar
17.Steinke, DE, Weir, BKA, Findlay, JM, Tanabe, T, Grace, M, et al. A trial of the 21-aminosteroid U74006F in a primate model of chronic cerebral vasospasm. Neurosurgery 1989; 24: 179–186.CrossRefGoogle Scholar
18.Fujita, Y, Shingu, T, Gi, H, Araki, O, Matsunaga, M, et al. Mechanism of cerebral vasospasm induced by oxyhemoglobin. In: Brock, M, ed. Modern Neurosurgery I. Berlin, Springer-Verlag, 1982; 359–374.CrossRefGoogle Scholar
19.Misra, HP, Fridovich, I.The generation of superoxide radicals during the autoxidation of hemoglobin. J Biol Chem 1972; 247: 6960–6962.CrossRefGoogle ScholarPubMed
20.Wever, R, Oudega, B, Van Gelder, BF.Generation of superoxide radicals during the autooxidation of mammalian oxyhemoglobin. Biochem Biophys Acta 1973; 302: 475–478.Google Scholar
21.Halliwell, B, Gutteridge, JMS, eds. Free Radicals in Biology and Medicine. Oxford, Clarendon Press 1985: 139–189.Google Scholar
22.Koide, T, Noda, Y, Hata, S, Sugioka, K, Kobayashi, S, et al. Contraction of the canine basilar artery following linoleic, arachi-donic, 13-hydroperoxylinoleic, or 15-hydroperoxyarachidonic acid (41294). Proc Soc Exper Biol Med 1981; 168: 399–402.CrossRefGoogle Scholar
23.Koide, T, Neichi, T, Takato, M, Matsushita, H, Sugioka, K, et al. Possible mechanisms of 15-hydroperoxyarachidonic acid-induced contraction of the canine basilar artery in vitro. J Pharmacol Exp Ther 1982; 221: 481–488.Google ScholarPubMed
24.Suzuki, N, Nakamura, T, Imabayashi, S, Ishikawa, Y, Sasaki, T, et al. Identification of 5-hydroxy eicosatetraenoic acid in cere-brospinal fluid after subarachnoid hemorrhage. J Neurochem 1983; 41: 1186–1189.CrossRefGoogle Scholar
25.Sakaki, S, Kuwabara, H, Ohta, S.Biological defence mechanism in the pathogenesis of prolonged cerebral vasospasm in the patients with ruptured intracranial aneurysms. Stroke 1986; 17: 196–202.CrossRefGoogle Scholar
26.Watanabe, T, Sasaki, T, Asano, T, Takakura, K, Sano, K, et al. Changes in glutathione peroxidase and lipid peroxides in cere-brospinal fluid and serum after subarachnoid hemorrhage – with special reference to the occurrence of cerebral vasospasm. Neurol Med Chir (Tokyo) 1988; 28: 645–649.CrossRefGoogle Scholar
27.Sakaki, S, Ohta, S, Nakamura, H, Takeda, S.Free radical reaction and biological defense mechanism in the pathogenesis of prolonged vasospasm in experimental subarachoid hemorrhage. J Cereb Blood Flow Metab 1988; 8: 1–8.CrossRefGoogle Scholar
28.Tokoro, K.Cerebral vasospasm and lipoperoxide damage – morphological localization and measurement of lipoperoxide in pro-longed cerebral vasospam. No Shinkei Geka 1984; 12: 1049–1058.Google Scholar
29.Feher, J, Csomos, G, Verecki, A, eds. Free Radical Reactions in Medicine. Berlin, Springer-Verlag, 1987; 18–39.Google Scholar
30.Okamoto, S, Handa, H, Toda, N.Role of intrinsic arachidonate metabolites in the vascular action of erythrocyte breakdown products. Stroke 1984; 15: 60–64.CrossRefGoogle ScholarPubMed
31.Toda, N.Mechanisms of contracting action of oxyhemoglobin in isolated monkey and dog cerebral arteries. Am J Physiol 1990; 258: H57–H63.Google ScholarPubMed
32.Macdonald, RL, Weir, BKS, Runzer, TD, Grace, MGA, Poznansky, MJ.Effect of intrathecal superoxide dismutase and catalase on oxyhemoglobin-induced vasospasm in monkeys. Neurosurgery 1992 (in press).Google ScholarPubMed
33.Halliwell, B, Gutteridge, JMC.Oxygen radicals and the nervous system. Trends Neurosci 1985; 8: 22–26.CrossRefGoogle Scholar
34.Clemens, MR, Einsele, H, Remmer, H, Waller, HD.An essential requirement for ferrous-haemoglobin in the hydrogen peroxide stimulated oxidation of red blood cell membrane lipids. Biochem Pharmacol 1985; 34: 1339–1341.CrossRefGoogle ScholarPubMed
35.Fujita, Y, Shingu, T, Yamada, K, Araki, O, Matsunaga, M, et al. Noxious free radicals derived from oxyhemoglobin as a cause of prolonged vasospasm. Neurol Med Chir (Tokyo) 1980; 20: 137–144.CrossRefGoogle ScholarPubMed
36.Kuwabara, H, Sakaki, S, Nakano, K, Yano, M, Hatakeyama, T, et al. A study on the changes of superoxide dismutase activity and the amount of lipid peroxide in patients with ruptured intracranial aneurysms with special reference to the pathogenesis of vasospasm. Nosochu no Geka 1982; 11: 159–164.Google Scholar