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Magnetic Resonance Imaging and 31P Magnetic Resonance Spectroscopy Study of the Effect of Temperature on Ischemic Brain Injury

Published online by Cambridge University Press:  18 September 2015

Garnette R. Sutherland ,
Howard Lesiuk ,
Paul Hazendonk ,
James Peeling ,
Richard Buist ,
Piotr Kozlowski ,
Andrzej Jazinski  and
John K. Saunders
Garnette R. Sutherland
Affiliation:
Departments of Surgery (Neurosurgery), Pharmacology, and Radiology, The University of Manitoba; Department of Chemistry, University of Winnipeg, Winnipeg; and Division of Biological Sciences, National Research Council of Canada, Ottawa
Howard Lesiuk
Affiliation:
Departments of Surgery (Neurosurgery), Pharmacology, and Radiology, The University of Manitoba; Department of Chemistry, University of Winnipeg, Winnipeg; and Division of Biological Sciences, National Research Council of Canada, Ottawa
Paul Hazendonk
Affiliation:
Departments of Surgery (Neurosurgery), Pharmacology, and Radiology, The University of Manitoba; Department of Chemistry, University of Winnipeg, Winnipeg; and Division of Biological Sciences, National Research Council of Canada, Ottawa
James Peeling*
Affiliation:
Departments of Surgery (Neurosurgery), Pharmacology, and Radiology, The University of Manitoba; Department of Chemistry, University of Winnipeg, Winnipeg; and Division of Biological Sciences, National Research Council of Canada, Ottawa
Richard Buist
Affiliation:
Departments of Surgery (Neurosurgery), Pharmacology, and Radiology, The University of Manitoba; Department of Chemistry, University of Winnipeg, Winnipeg; and Division of Biological Sciences, National Research Council of Canada, Ottawa
Piotr Kozlowski
Affiliation:
Departments of Surgery (Neurosurgery), Pharmacology, and Radiology, The University of Manitoba; Department of Chemistry, University of Winnipeg, Winnipeg; and Division of Biological Sciences, National Research Council of Canada, Ottawa
Andrzej Jazinski
Affiliation:
Departments of Surgery (Neurosurgery), Pharmacology, and Radiology, The University of Manitoba; Department of Chemistry, University of Winnipeg, Winnipeg; and Division of Biological Sciences, National Research Council of Canada, Ottawa
John K. Saunders
Affiliation:
Departments of Surgery (Neurosurgery), Pharmacology, and Radiology, The University of Manitoba; Department of Chemistry, University of Winnipeg, Winnipeg; and Division of Biological Sciences, National Research Council of Canada, Ottawa
*
Department of Pharmacology, The University of Manitoba, 770 Bannatyne Avenue, Winnipeg, Manitoba, Canada R3E 0W3
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Abstract:

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Transient forebrain ischemia was induced in rats whose brain temperature was 31, 33, 35, 38, or 40°C. The development of regional injury was followed using magnetic resonance (MR) imaging, with the ultimate extent of neuronal injury quantified histopathologically. Animals in the hypothermic groups showed minimal changes in MR images over 4 days; normothermic animals snowed intensity enhancement attributed to progressive edema developing in the striatum and, later, in the hippocampus. Ischemia at 40°C resulted in widespread edema formation by I day post-ischemia; animals in this group did not survive beyond 30 hours. Histopathological analysis at 4 days (1 day for the hyperthermic group) post-ischemia showed that neuronal damage in the normothermic group was confined to the hippocampus and striatum. Minimal damage was found in the hypothermic groups; damage in the hyperthermic group was severe throughout the forebrain. There were no differences in the pre-ischemia 31P MR spectra for the different groups. During ischemia, the increase in intensity of the Pi peak and the fall in tissue pH increased with temperature in the order hypothermic < normothermic < hyperthermic group of animals. Post-ischemia energy recovery was similar in all groups, while pH recovered more rapidly in hypothermic animals.

Type
Articles
Information
Canadian Journal of Neurological Sciences , Volume 19 , Issue 3 , August 1992 , pp. 317 - 325
Copyright
Copyright © Canadian Neurological Sciences Federation 1992

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