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Localization of Nestin in Amygdaloid Kindled Rat: An Immunoelectron Microscopic Study

Published online by Cambridge University Press:  02 December 2014

Toshitaka Nakagawa ,
Osamu Miyamoto ,
Najma A. Janjua ,
Roland N. Auer ,
Seigo Nagao  and
Toshifumi Itano
Toshitaka Nakagawa
Affiliation:
Departments of Neurobiology, Kagawa Medical University, Miki, Kagawa, Japan
Osamu Miyamoto
Affiliation:
Departments of Neurobiology, Kagawa Medical University, Miki, Kagawa, Japan
Najma A. Janjua
Affiliation:
Departments of Neurobiology, Kagawa Medical University, Miki, Kagawa, Japan
Roland N. Auer
Affiliation:
Departments of Pathology and Clinical Neurosciences, Neuroscience Research Group, Faculty of Medicine, University of Calgary, Alberta, Canada
Seigo Nagao
Affiliation:
Department of Neurological Surgery, Kagawa Medical University, Miki, Kagawa, Japan
Toshifumi Itano
Affiliation:
Departments of Neurobiology, Kagawa Medical University, Miki, Kagawa, Japan
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Abstract

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Background:

Nestin is a class VI intermediate filament protein, expressed during early embryonic development in mammals. Postnatally, nestin and its mRNA are down-regulated and gradually disappear. Recently, nestin expression has been detected in the adult nervous system, and it has been suggested that this protein may be related to neurogenesis, although, its role in the mechanism of neurogenesis is not known.

Methods:

The present study examined the localization of nestin in CNS tissue of the amygdaloid kindled rat by light and electron microscopy.

Results:

Kindled animals showed nestin expression mainly in the piriform cortex and the perirhinal cortex. By light microscopy, nestin was shown to be expressed in astrocytes, neurons, and endothelial cells. Electron microscopy demonstrated nestin expression in endothelial cells, astrocytic perivascular end feet, the rare pericyte, neurons and oligodendrocytes.

Conclusion:

We conclude that epilepsy causes widespread nestin expression in many cell types in the CNS, including non-neural cells.

Type
Other
Information
Canadian Journal of Neurological Sciences , Volume 31 , Issue 4 , November 2004 , pp. 514 - 519
Copyright
Copyright © The Canadian Journal of Neurological 2004

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