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GPI-1046 Increases Presenilin-1 Expression and Restores NMDA Channel Activity

Published online by Cambridge University Press:  02 December 2014

Joseph P. Steiner ,
Kathryn B. Payne ,
Christopher Drummond Main ,
Sabrina D'Alfonso ,
Kirsten X. Jacobsen ,
T. Philip Hicks ,
William A. Staines  and
Michael O. Poulter
Joseph P. Steiner
Affiliation:
Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Kathryn B. Payne
Affiliation:
Neuroscience Research Institute, Department of Psychology, Carleton University
Christopher Drummond Main
Affiliation:
Neuroscience Research Institute, Department of Psychology, Carleton University
Sabrina D'Alfonso
Affiliation:
Neuroscience Research Institute, Department of Psychology, Carleton University
Kirsten X. Jacobsen
Affiliation:
Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa
T. Philip Hicks
Affiliation:
Department of Biology and Faculty of Graduate Studies, Lakehead University, Thunder Bay
William A. Staines
Affiliation:
Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa
Michael O. Poulter*
Affiliation:
Neuroscience Research Institute, Department of Psychology, Carleton University
*
Molecular Brain Research Group, Robarts Research Institute, 100 Perth Drive Box 5015, London Ontario, N6A 5K8, Canada
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Abstract

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Background:

Previously we showed that 6-hydroxydopamine lesions of the substantia nigra eliminate corticostriatal LTP and that the neuroimmunolophilin ligand (NIL), GPI-1046, restores LTP.

Methods:

We used cDNA microarrays to determine what mRNAs may be over- or under-expressed in response to lesioning and/or GPI-1046 treatment. Patch clamp recordings were performed to investigate changes in NMDA channel function before and after treatments.

Results:

We found that 51 gene products were differentially expressed. Among these we found that GPI-1046 treatment up-regulated presenilin-1 (PS-1) mRNA abundance. This finding was confirmed using QPCR. PS-1 protein was also shown to be over-expressed in the striatum of lesioned/GPI-1046-treated rats. As PS-1 has been implicated in controlling NMDA-receptor function and LTP is reduced by lesioning we assayed NMDA mediated synaptic activity in striatal brain slices. The lesion-induced reduction of dopaminergic innervation was accompanied by the near complete loss of NDMA receptor-mediated synaptic transmission between the cortex and striatum. GPI-1046 treatment of the lesioned rats restored NMDA-mediated synaptic transmission but not the dopaminergic innervation. Restoration of NDMA channel function was apparently specific as the sodium channel current density was also reduced due to lesioning but GPI-1046 did not reverse this effect. We also found that restoration of NMDA receptor function was also not associated with either an increase in NMDA receptor mRNA or protein expression.

Conclusion:

As it has been previously shown that PS-1 is critical for normal NMDA receptor function, our data suggest that the improvement of excitatory neurotransmission occurs through the GPI-1046-induced up-regulation of PS-1.

Type
Original Article
Information
Canadian Journal of Neurological Sciences , Volume 37 , Issue 4 , July 2010 , pp. 457 - 467
Copyright
Copyright © The Canadian Journal of Neurological 2010

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