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E-Selectin Expression Increased in Human Ruptured Cerebral Aneurysm Tissues

Published online by Cambridge University Press:  02 December 2014

Wenqing Jia ,
Rong Wang ,
Jizong Zhao ,
Isabelle Yisha Liu ,
Dong Zhang ,
Xuejiang Wang  and
Xiaodi Han
Wenqing Jia
Affiliation:
Department of Neurosurgery, Capital Medical University
Rong Wang
Affiliation:
Department of Neurosurgery, Capital Medical University
Jizong Zhao*
Affiliation:
Department of Neurosurgery, Capital Medical University
Isabelle Yisha Liu
Affiliation:
School of Medicine, Washington University, St. Louis, Missouri
Dong Zhang
Affiliation:
Department of Neurosurgery, Capital Medical University
Xuejiang Wang
Affiliation:
Department of Pathology, TianTan Hospital, School of Basic Medical Sciences, Capital Medical University
Xiaodi Han
Affiliation:
Department of Neurosurgery, Beijing Tiantan Puhua Hospital, Beijing China Department of Neurosurgery, University of Rochester Medical Center, New York, New York, USA
*
Department of Neurosurgery, TianTan Hospital, Capital Medical University, Beijing China, 100050.
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Abstract

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Objectives:

The purpose of the present study is to investigate the expression of inflammation factor endothelial-leukocyte adhesion molecule (E-selectin, CD62E) in cerebral aneurysm walls and its relationship with aneurysm rupture.

Methods:

Cerebral aneurysm tissue samples were collected at the time of surgical clipping of nine patients with history of subarachnoid hemorrhage, and then compared with control artery tissues from the superficial temporal arteries (STA) of five patients with intracranial tumors. Immunohistochemistry (IHC) was performed to reveal and localize E-selectin expression in the aneurysms and artery tissues. Western blot analysis was used to relatively quantify the level of E-selectine protein expression in cerebral aneurysms when compared with normal arteries.

Results:

E-selectin was detected in the wall of all the aneurysm tissue samples and was rarely found in normal control arteries by IHC, and it was concentrated in proliferating and disorganized epithelia cells. Moreover, with the Western blot method, the E-selectin protein level increased significantly in aneurysm tissues compared to normal STA.

Conclusions:

E-selectin might be an important factor involved in the process of cerebral aneurysm formation and rupture, by promoting inflammation and weakening cerebral artery walls.

Type
Original Articles
Information
Canadian Journal of Neurological Sciences , Volume 38 , Issue 6 , November 2011 , pp. 858 - 862
Copyright
Copyright © The Canadian Journal of Neurological 2011

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