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Distribution of Dopamine in 35 Subregions of the Rat Caudate-Putamen: A High Performance Liquid Chromatography with Electrochemical Detection Analysis

Published online by Cambridge University Press:  18 September 2015

Thérèse Di Paolo ,
Michel Daigle  and
André Dupont
Thérèse Di Paolo*
Affiliation:
the Department of Molecular Endocrinology, Le Centre Hospitalier de l’Université Laval, Quebec GI V 4G2, Canada
Michel Daigle
Affiliation:
the Department of Molecular Endocrinology, Le Centre Hospitalier de l’Université Laval, Quebec GI V 4G2, Canada
André Dupont
Affiliation:
the Department of Molecular Endocrinology, Le Centre Hospitalier de l’Université Laval, Quebec GI V 4G2, Canada
*
Department of Molecular Endocrinology, Le Centre Hospitalier de l’Université Laval, Quebec, P.Q., Canada GI V4G2
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Topographical variations in the endogenous content of dopamine were studied in the rat caudate-putamen. For this purpose, a high performance liquid chromatography with electrochemical detection procedure for the determination of norepinephrine, epinephrine and dopamine in nervous tissue samples is described. Tissue preparation required only homogenization in acidic solution and centrifugation prior to application to the chromatograph. Detection limits in the low picogram range were found for the catecholamines separated. This assay was used in combination with a micropunch dissection technique. The performance of this analytical technique is illustrated by the detailed mapping of dopamine content in 35 subregions of the rat caudate-putamen. The distribution of dopamine varies along the major axis of the nucleus.

Type
Research Article
Information
Canadian Journal of Neurological Sciences , Volume 9 , Issue 4 , November 1982 , pp. 421 - 427
Copyright
Copyright © Canadian Neurological Sciences Federation 1982

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