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Cytochemical Localization of Adenylate Cyclase in Broken Cell Preparations of the Cerebral Cortex

Published online by Cambridge University Press:  18 September 2015

S. W. French ,
D. S. Palmer  and
M. Caldwell
S. W. French*
Affiliation:
Departments of Pathology, University of British Columbia, Canada and the University of California School of Medicine, Davis, California, and the Veterans Administration Hospital, Martinez, California
D. S. Palmer
Affiliation:
Departments of Pathology, University of British Columbia, Canada and the University of California School of Medicine, Davis, California, and the Veterans Administration Hospital, Martinez, California
M. Caldwell
Affiliation:
Departments of Pathology, University of British Columbia, Canada and the University of California School of Medicine, Davis, California, and the Veterans Administration Hospital, Martinez, California
*
Veterans Administration Hospital, 150 Muir Road, Martinez, Ca. 94553, U.S.A.
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Broken cell preparations derived from rat cerebral cortical grey matter were studied cytochemically to localize adenylate cyclase (AC) activity in subcellular organelle membranes. AC activity was localized by visualizing reaction product in brain particulate fractions by electron microscopy. Activity was found in the endoplasmic reticulum, on the inside of the inner mitochondrial membrane and on both leaflets of the nuclear membrane. Reaction product was found in the postsynaptic density area of most synapses. The reaction product tended to be more prominent in the presence of flouride. A synaptosome-rich fraction was shown to have NE stimulated AC activity which was blocked in vitro by both a α-and an β-blocker and in vivo by propranolol.

Type
Research Article
Information
Canadian Journal of Neurological Sciences , Volume 5 , Issue 1 , February 1978 , pp. 33 - 40
Copyright
Copyright © Canadian Neurological Sciences Federation 1978

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