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Cannabinoids and Dopamine Receptors' Action on Calcium Current in Rat Neurons

Published online by Cambridge University Press:  02 December 2014

C. Vásquez ,
R. Navarro-Polanco ,
G. Hernández ,
J. Ruiz ,
D.G. Guerra ,
L.M. Baltazar ,
M. Huerta  and
X. Trujillo
C. Vásquez*
Affiliation:
Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colonia Villa de San Sebastián, Colima, Colima, C.P. 28040, México
R. Navarro-Polanco
Affiliation:
Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colonia Villa de San Sebastián, Colima, Colima, C.P. 28040, México
G. Hernández
Affiliation:
Facultad de Enfermeria, Universidad de Colima, Colonia Las Viboras, Colima, Colima, C.P. 28040, Mexico
J. Ruiz
Affiliation:
Facultad de Enfermeria, Universidad de Colima, Colonia Las Viboras, Colima, Colima, C.P. 28040, Mexico
D.G. Guerra
Affiliation:
Facultad de Enfermeria, Universidad de Colima, Colonia Las Viboras, Colima, Colima, C.P. 28040, Mexico
L.M. Baltazar
Affiliation:
Facultad de Medicina, Universidad de Colima, Colonia Las Viboras, Colima, Colima, C.P. 28040, Mexico
M. Huerta
Affiliation:
Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colonia Villa de San Sebastián, Colima, Colima, C.P. 28040, México
X. Trujillo
Affiliation:
Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colonia Villa de San Sebastián, Colima, Colima, C.P. 28040, México
*
Centro Universitario de Investigaciones Biomédicas, Avenida 25 de julio # 965, Universidad de Colima, Colonia Villa de San Sebastián, Colima, Colima, C.P. 28040, México
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Abstract:

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Objective:

To study the effects of cannabinoid, glutamate, and dopamine agonists and antagonists on the calcium current in rat sympathetic neurons.

Methods:

Calcium current was recorded using the whole-cell variant of the patch-clamp technique. After expression in neuronal membranes of the cannabinoid CB1, glutamate mGluR2, or dopamine D1 receptor (by microinjection of the relevant receptor's cDNA into the neuron's nucleus) agonists' and antagonists' effects were observed.

Results:

Applications of agonists of the expressed receptor (0.1-10 µM) decreased the calcium current. The calcium current was increased after application of cannabinoid antagonists (AM251 and AM630); these compounds thus act as inverse agonists in this preparation. Glutamate and dopamine antagonists had no effects on the calcium current by themselves. Combined application of cannabinoids and dopamine, but not glutamate, agonists produced a decrement in the calcium current that was bigger than either of the effects seen when one agonist was applied alone.

Conclusions:

These results suggest that cannabinoid with dopamine receptors have an interactive inhibitory effect on the calcium current in this preparation, indicating that within the nervous system, receptor interactions may be important in the regulation of ion-channel functions.

Résumé:

RÉSUMÉ: Objectif:

Étudier les effets d’agonistes et d’antagonistes de cannabinoïdes, du glutamate et de la dopamine sur le courant calcique dans des neurones sympathiques de rat.

Méthodes:

Le courant calcique a été enregistré au moyen de la technique patch-clamp sur cellules intactes. Des effets agonistes et antagonistes ont été observés après expression dans les membranes neuronales du récepteur cannabinoïde de type CB1, du récepteur métabotropique du glutamate mGluR2 ou du récepteurs D1 de la dopamine par micro injection de l’ADNc correspondant au récepteur dans le noyau du neurone.

Résultats:

L’application d’agonistes du récepteur exprimé (0,1 à 10 mmol) diminuait le courant calcique. Le courant calcique était augmenté suite à l’application d’antagonistes cannabinoïdes tels l’AM251 et l’AM630. Ces substances agissent donc comme des agonistes inverses dans cette préparation. Les antagonistes du glutamate et de la dopamine n’avaient pas d’effet sur le courant calcique par eux-mêmes. Une application combinée d’agonistes de cannabinoïdes et de dopamine diminuait davantage le courant calcique que l’application de chacun d’eux seul, ce qui n’était pas le cas des agonistes du glutamate.

Conclusions:

Ces résultats suggèrent un effet inhibiteur interactif des récepteurs cannabinoïdes et des récepteurs dopaminergiques sur le courant calcique dans cette préparation. Ceci indique que, dans le système nerveux, l’interaction de récepteurs pourrait être importante dans la régulation des fonctions des canaux ioniques.

Type
Experimental Neurosciences
Information
Canadian Journal of Neurological Sciences , Volume 32 , Issue 4 , November 2005 , pp. 529 - 537
Copyright
Copyright © The Canadian Journal of Neurological 2005

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