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TREHALASE ACTIVITY IN THE SILK GLANDS OF THE SILKWORM, BOMBYX MORI (LEPIDOPTERA: BOMBYCIDAE)

Published online by Cambridge University Press:  31 May 2012

Shuya Shimada
Shuya Shimada
Affiliation:
The 2nd Research Laboratory, Tokushima Factory, Otsuka Pharmaceutical Co., Ltd., Kawauchi-cho, Tokushima, Japan
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Abstract

(1) Crude extracts prepared from the silk glands of the silkworm, Bombyx mori L. contain trehalase activity. (2) Trehalase in the silk glands has a pH of 5.5 and a Km of 0.71 mM. The activity of the enzyme is inhibited by divalent cations such as Mn, Cu, and Zn. (3) By histochemical methods, it is shown that trehalase is localized in the periphery of the silk gland cells, especially in the tunica propria and tunica intima. (4) Trehalase activity is low in fifth instar and increases greatly in spinning stages, after which the activity decreases.

Type
Articles
Information
The Canadian Entomologist , Volume 107 , Issue 12 , December 1975 , pp. 1311 - 1314
Copyright
Copyright © Entomological Society of Canada 1975

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