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SPECTROPHOTOMETRIC DETERMINATION OF LARVAL INGESTION RATES IN THE SPRUCE BUDWORM (LEPIDOPTERA: TORTRICIDAE)

Published online by Cambridge University Press:  31 May 2012

Arthur Retnakaran
Arthur Retnakaran
Affiliation:
Forest Pest Management Institute, Canadian Forestry Service, Sault Ste. Marie, Ontario P6A 5M7
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Abstract

The food intake of fourth, fifth, and sixth instar spruce budworm, Choristoneura fumiferana Clemens, was investigated by feeding larvae for 24 h artificial diet incorporating amaranth dye. Amaranth was selected because it followed Beer's law over a wide concentration range, mixed well with the meridic diet, could be extracted in ice-cold water, was not absorbed by larval tissue, had minimal feeding deterrence as well as marginal adverse chronic effects on the larvae, and finally had negligible effect on ingestion rate over a 24 h period. The results indicated that 6th instars consumed 20 times and 5th instars 3 times as much as 4th instars, but when compared on a body weight basis the ingestion rate was similar in all three instars. Temperature and photoperiod influenced the rate of food intake. The application of these findings in assessing defoliation to the forests as well as estimating dosage of pesticides for control of this species are discussed.

Résumé

On a étudié la quantité de nourriture ingérée par les larves de la tordeuse des bourgeons de l'épinette (Choristoneura fumiferana [Clem.] des quatrième, cinquième et sixième stades soumises pendant 24 heures à un régime artificiel coloré à l'amarante. On a choisi l'amarante parce que c'est un colorant qui obéit à la loi de Beer dans un large éventail de concentrations, qu'il se mélange bien avec les constituants du régime, qu'il peut être extrait à l'eau glacée, mais n'est pas absorbé par les tissus des larves, qu'il ne dissuade que très peu les larves et ne produit sur elles qu'un faible effet chronique nuisible et, enfin, qu'il n'a qu'un effet négligeable sur la qu antité de nourriture ingérée en 24 heures. D'après les résultats, les larves des sixième et cinquième ont ingéré respectivement 20 et 3 fois plus de nourriture que les larves du quatrième stade, mais, en fonction du poids corporel, la proportion ingérée était semblable dans les trois cas. La température et la photopériode ont influé sur la quantité de nourriture ingérée. On discute de l'application de ces résultats à l'évaluation de la défoliation des forêts ainsi qu'à l'estimation de la dose de pesticides à utiliser contre la tordeuse.

Type
Articles
Information
The Canadian Entomologist , Volume 115 , Issue 1 , January 1983 , pp. 31 - 40
Copyright
Copyright © Entomological Society of Canada 1983

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