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SOLUTION SEPARATION AND MAXIMUM LIKELIHOOD DENSITY ESTIMATES OF HEMLOCK LOOPER (LEPIDOPTERA: GEOMETRIDAE) EGGS IN MOSS
Published online by Cambridge University Press: 31 May 2012
Abstract
Counting hemlock looper (Lambditm fiscellaria lugnbrosa) eggs attached to moss samples is tedious and inaccurate. Moss samples treated with a 0.5% NaOH solution for 1 minute releases the eggs, which can be washed and filtered out of the moss. Other debris can be separated through differential flotation with 15% NaCl solution. Three such washings provide data for analysis with the maximum likelihood method, producing density estimates with low-standard errors.
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