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Selection of Aedes aegypti (Diptera: Culicidae) strains that are susceptible or refractory to Dengue-2 virus

Published online by Cambridge University Press:  23 April 2013

Paola A. Caicedo ,
Olga L. Barón ,
Mauricio Pérez ,
Neal Alexander ,
Carl Lowenberger  and
Clara B. Ocampo
Paola A. Caicedo
Affiliation:
Centro Internacional de Entrenamiento e Investigaciones Médicas (CIDEIM), Carrera 125 No. 19–225, Santiago de Cali, Colombia
Olga L. Barón
Affiliation:
Centro Internacional de Entrenamiento e Investigaciones Médicas (CIDEIM), Carrera 125 No. 19–225, Santiago de Cali, Colombia
Mauricio Pérez
Affiliation:
Centro Internacional de Entrenamiento e Investigaciones Médicas (CIDEIM), Carrera 125 No. 19–225, Santiago de Cali, Colombia
Neal Alexander
Affiliation:
Centro Internacional de Entrenamiento e Investigaciones Médicas (CIDEIM), Carrera 125 No. 19–225, Santiago de Cali, Colombia
Carl Lowenberger
Affiliation:
Department of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, British Columbia V5A 1S6, Canada
Clara B. Ocampo*
Affiliation:
Centro Internacional de Entrenamiento e Investigaciones Médicas (CIDEIM), Carrera 125 No. 19–225, Santiago de Cali, Colombia
*
1Corresponding author (e-mail: [email protected]).
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Abstract

The vector competence (VC) of Aedes aegypti (Linnaeus) (Diptera: Culicidae) varies geographically and is affected by both genetic and environmental factors. Understanding the molecular mechanisms that influence VC may help develop novel control strategies. The selection of susceptible and refractory strains is the first step in this process. We collected immature A. aegypti in the field and established strains that were susceptible and refractory to Dengue-2 virus by isofamily selection through several generations. Infection was detected by immunofluorescence of head or midgut tissues to determine infection barriers and the % of VC by tissue. We selected three strains: Susceptible (Cali-S) (96.4% susceptible at F19), Refractory with a midgut escape barrier (Cali-MEB) (44.1% refractory at F15), and Refractory with a midgut infection barrier (Cali-MIB) (40% refractory at F16). The effects of the infection were measured using Kaplan–Meier survival rates over the first seven generations. All selected strains showed a similar decrease in survival and in the number of eggs laid/female through the seven generations, suggesting that changes were a result of the selection process rather than the virus infection. The results of this study suggest that VC is associated with multiple genes, which have additive effects on susceptibility.

Résumé

La compétence vectorielle (CV) d’Aedes aegypti (Linnaeus) (Diptera: Culicidae) varie géographiquement et est influencée par des facteurs génétiques et environnementaux. Comprendre les mécanismes moléculaires qui affectent la CV pourrait aider à développer de nouvelles stratégies de contrôle des moustiques. La sélection de souches susceptibles et réfractaires est la première étape dans ce processus. Nous avons recueilli des A. aegypti immatures sur le terrain et des souches susceptibles et réfractaires au virus dengue-2 on été établies par la sélection d'isofamilles sur plusieurs générations. L'infection a été détectée par immunofluorescence sur des tissus de la tête ou de l'intestin moyen pour déterminer les barrières d'infection et le % de CV par tissu. Nous avons sélectionné trois souches: Susceptible (Cali-S) (96,4% sensibles à F19), Réfractaire avec une barrière contre la dissémination dans l'intestin moyen (Cali-MEB) (44,1% réfractaire à F15) et Réfractaires avec une barrière contre l'infection dans l'intestin moyen (Cali-MIB) (40% réfractaire à F16). Les effets de l'infection ont été mesurés en utilisant les taux de survie de Kaplan–Meier au cours des sept premières générations. Toutes les souches sélectionnées ont montré une diminution similaire du taux de survie et du nombre d'oeufs pondus/femelle à travers les sept générations, ce qui suggère que les changements sont le résultat du processus de sélection plutôt que l'infection par le virus. Les résultats de cette étude suggèrent que la CV est associée à de multiples gènes qui ont des effets additifs sur la susceptibilité.

Type
Physiology, Biochemistry, Development, and Genetics
Information
The Canadian Entomologist , Volume 145 , Issue 3 , June 2013 , pp. 273 - 282
Copyright
Copyright © Entomological Society of Canada 2013 

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