Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-22T22:58:09.010Z Has data issue: false hasContentIssue false
  • English
  • Français

Resolving the taxonomy of the Merodon dobrogensis species subgroup (Diptera: Syrphidae), with the description of a new species

Published online by Cambridge University Press:  19 December 2019

Ljiljana Šašić Zorić ,
Jelena Ačanski Open the ORCID record for Jelena Ačanski [Opens in a new window] ,
Ante Vujić ,
Gunilla Ståhls ,
Mihajla Djan  and
Snežana Radenković
Ljiljana Šašić Zorić
Affiliation:
BioSense Institute, University of Novi Sad, Dr Zorana Đinđića 1, 21000Novi Sad, Serbia
Jelena Ačanski*
Affiliation:
BioSense Institute, University of Novi Sad, Dr Zorana Đinđića 1, 21000Novi Sad, Serbia
Ante Vujić
Affiliation:
Faculty of Sciences, Department of Biology and Ecology, University of Novi Sad, Trg Dositeja Obradovića 3, 21000Novi Sad, Serbia
Gunilla Ståhls
Affiliation:
Zoology Unit, Finnish Museum of Natural History, University of Helsinki, PO Box 17, 00014Helsinki, Finland
Mihajla Djan
Affiliation:
Faculty of Sciences, Department of Biology and Ecology, University of Novi Sad, Trg Dositeja Obradovića 3, 21000Novi Sad, Serbia
Snežana Radenković
Affiliation:
Faculty of Sciences, Department of Biology and Ecology, University of Novi Sad, Trg Dositeja Obradovića 3, 21000Novi Sad, Serbia
*
*Corresponding author. Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The taxonomy of Merodon dobrogensis Bradescu, 1982 (Diptera: Syrphidae) species subgroup was reviewed. Multiple data sources (morphology, geometric morphometry of wings and surstylus, molecular data, and distributional data) were used to investigate the species subgroup in the manner of integrative taxonomy. Merodon dobrogensis Bradescu, 1982 and M. puniceus Vujić, Radenković, and Pérez-Bañón, 2011 are supported as distinct species belonging to the M. dobrogensis species complex within the M. dobrogensis species subgroup. Additionally, evidence is presented for the description of a new species, M. rojoi Radenković and Vujić new species, with a distribution in mainland Greece, the Greek island Euboea, and the Peloponnese. A short diagnosis is provided for the M. aureus species group, the M. dobrogensis species subgroup, and the newly defined M. dobrogensis species complex, in addition to a description of the new species, with drawings and photographs of adult morphology.

Type
Research Papers
Information
The Canadian Entomologist , Volume 152 , Issue 1 , February 2020 , pp. 36 - 59
Copyright
© 2019 Entomological Society of Canada 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ačanski, J., Vujić, A., Djan, M., Obreht Vidaković, D., Ståhls, G., and Radenković, S. 2016. Defining species boundaries in the Merodon avidus complex (Diptera, Syrphidae) using integrative taxonomy, with the description of a new species [online]. European Journal of Taxonomy, 237: 1–25. https://doi.org/10.5852/ejt.2016.237.Google Scholar
Belshaw, R., Lopez-Vaamonde, C., Degerli, N., and Quicke, D.L. 2001. Paraphyletic taxa and taxonomic chaining: evaluating the classification of braconine wasps (Hymenoptera: Braconidae) using 28S D2-3 rDNA sequences and morphological characters. Biological Journal of the Linnean Society, 73: 411–424. https://doi.org/10.1111/j.1095-8312.2001.tb01370.x.CrossRefGoogle Scholar
Bookstein, F.L. 1997. Landmark methods for forms without landmarks: morphometrics of group differences in outline shape. Medical Image Analysis, 1: 225–243. https://doi.org/10.1016/S1361-8415(97)85012-8.CrossRefGoogle ScholarPubMed
Bradescu, V. 1982. Deux espèces nouvelles des genres Cheilosia Meigen et Merodon Meigen (Diptera, Syrphidae). Revue Roumaine de Biologie – Serie de Biologie Animale, 27: 11–15.Google Scholar
Chen, H., Rangasamy, M., Tan, S.Y., Wang, H., and Siegfried, B.D. 2010. Evaluation of five methods for total DNA extraction from western corn rootworm beetles. Public Library of Science One, 5: e11963. https://doi.org/10.1371/journal.pone.0011963.Google ScholarPubMed
Chroni, A., Grković, A., Ačanski, J., Vujić, A., Radenković, S., Veličković, N., et al. 2018. Disentangling a cryptic species complex and defining new species within the Eumerus minotaurus group (Diptera: Syrphidae), based on integrative taxonomy and Aegean palaeogeography. Contributions to Zoology, 87: 197–225.CrossRefGoogle Scholar
Folmer, O., Black, M., Hoeh, W., Lutz, R., and Vrijenhoek, R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3: 294–299. Available from https://www.mbari.org/wp-content/uploads/2016/01/Folmer_94MMBB.pdf [accessed 27 March 2019].Google ScholarPubMed
Goloboff, P. (1999). NONA computer program. Published by the author, Tucuman, Argentina.Google Scholar
Gorrochotegui-Escalante, N., Munoz, M.D.L., Fernandez-Salas, I., Beaty, B.J., and Black, W.C. 2000. Genetic isolation by distance among Aedes aegypti populations along the northeastern coast of Mexico. The American Journal of Tropical Medicine and Hygiene, 62: 200–209. https://doi.org/10.4269/ajtmh.2000.62.200.CrossRefGoogle Scholar
Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41: 95–98. Available from www.mbio.ncsu.edu/BioEdit/bioedit.html [accessed 16 October 2019].Google Scholar
Hijmans, R.J., Cameron, S.E., Parra, J.L., Jones, P.G., and Jarvis, A. 2005. Very high resolution interpolated climate surfaces for global land areas. International Journal of Climatology, 25: 1965–1978.CrossRefGoogle Scholar
Klingenberg, C.P. 2011. MorphoJ: an integrated software package for geometric morphometrics. Version 2.0. Molecular Ecology Resources, 11: 353–357. https://doi.org/10.1111/j.1755-0998.2010.02924.x.CrossRefGoogle Scholar
Kočiš Tubić, N., Ståhls, G., Ačanski, J., Djan, M., Obreht Vidaković, D., Hayat, R., et al. 2018. An integrative approach in the assessment of species delimitation and structure of the Merodon nanus species group (Diptera: Syrphidae). Organisms Diversity & Evolution, 18: 479–497. https://doi.org/10.1007/s13127-018-0381-7.CrossRefGoogle Scholar
Kumar, S., Stecher, G., and Tamura, K. 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33: 1870–1874. https://doi.org/10.1093/molbev/msw054.CrossRefGoogle ScholarPubMed
Librado, P. and Rozas, J. 2009. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25: 1451–1452. https://doi.org/10.1093/bioinformatics/btp187.CrossRefGoogle ScholarPubMed
Mantel, N.A. 1967. The detection of disease clustering and a generalized regression approach. Cancer Research, 27: 209–220.Google Scholar
Marcos-García, M.Á., Vujić, A., and Mengual, X. 2007. Revision of Iberian species of the genus Merodon (Diptera: Syrphidae). European Journal of Entomology, 104: 531–572. Available from https://www.eje.cz/pdfs/eje/2007/03/21.pdf [accessed 12 March 2019].CrossRefGoogle Scholar
Mengual, X., Ståhls, G., Vujić, A., and Marcos-García, M.Á. 2006. Integrative taxonomy of Iberian Merodon species (Diptera: Syrphidae). Zootaxa, 1377: 1–26.Google Scholar
Miller, M.A., Pfeiffer, W., and Schwartz, T. 2010. Creating the CIPRES science gateway for inference of large phylogenetic trees. In Proceedings of the Gateway Computing Environments Workshop (GCE), Institute of Electrical and Electronics Engineers, New Orleans, Louisiana, United States of America. Pp. 1–8. https://doi.org/10.1109/GCE.2010.5676129.CrossRefGoogle Scholar
Nedeljković, Z., Ačanski, J., Djan, M., Obreht-Vidaković, D., Ricarte, A., and Vujić, A. 2015. An integrated approach to delimiting species borders in the genus Chrysotoxum Meigen, 1803 (Diptera: Syrphidae), with description of two new species. Contributions to Zoology, 84: 285–304.CrossRefGoogle Scholar
Nedeljković, Z., Ačanski, J., Vujić, A., Obreht, D., Djan, M., Ståhls, G., and Radenković, S. 2013. Taxonomy of Chrysotoxum festivum Linnaeus, 1758 (Diptera: Syrphidae) – an integrative approach. Zoological Journal of the Linnean Society, 169: 84–102. https://doi.org/10.1111/zoj.12052.CrossRefGoogle Scholar
Nixon, K.C. 2008. ASADO, version 1.85 TNT-MrBayes slaver version 2; mxram 200 (vl 5.30). Available from www.diversityoflife.org/winclada [accessed 24 April 2018].Google Scholar
Petanidou, T., Vujić, A., and Ellis, W.N. 2011. Hoverfly diversity (Diptera: Syrphidae) in a Mediterranean scrub community near Athens, Greece. Annales de la Société Entomologique de France, 47: 168–175. https://doi.org/10.1080/00379271.2011.10697709.CrossRefGoogle Scholar
Quantum GIS Development Team. 2012. Quantum GIS geographic information system. Version 2.12.3 [online]. Available from http://qgis.osgeo.org [accessed 7 October 2019].Google Scholar
Radenković, S., Šašić Zorić, L., Djan, M., Obreht Vidaković, D., Ačanski, J., Ståhls, G., et al. 2018. Cryptic speciation in the Merodon luteomaculatus complex (Diptera: Syrphidae) from the eastern Mediterranean. Journal of Zoological Systematics and Evolutionary Research, 56: 170–191. https://doi.org/10.1111/jzs.12193.CrossRefGoogle Scholar
Radenković, S., Vujić, A., Ståhls, G., Pérez-Bañón, C., Rojo, S., Petanidou, T., and Šimić, S. 2011. Three new cryptic species of the genus Merodon Meigen (Diptera: Syrphidae) from the island of Lesvos (Greece). Zootaxa, 2735: 35–56. http://doi.org/10.11646/zootaxa.2735.1.5.CrossRefGoogle Scholar
Rodríguez, F., Oliver, J.L., Marín, A., and Medina, J.R. 1990. The general stochastic model of nucleotide substitution. Journal of Theoretical Biology, 142: 485–501. https://doi.org/10.1016/S0022-5193(05)80104-3.CrossRefGoogle ScholarPubMed
Rohlf, F.J. 2017a. tpsDig2 version 2.30. Available from https://life.bio.sunysb.edu/morph/morphmet/tpsdig2w32.exe [accessed 20 March 2019].Google Scholar
Rohlf, F.J. 2017b. tpsRelw version 1.68. Available from https://life.bio.sunysb.edu/morph/morphmet/tpsrelww32.exe [accessed 20 March 2019].Google Scholar
Rohlf, F.J. and Slice, D.E. 1990. Extensions of the Procrustes method for the optimal superimposition of landmarks. Systematic Biology, 39: 40–59. https://doi.org/10.2307/2992207.Google Scholar
Rosenberg, M.S. and Anderson, C.D. 2011. PASSaGE: pattern analysis, spatial statistics and geographic exegesis. Version 2. Methods in Ecology and Evolution, 2: 229–232. https://doi.org/10.1111/j.2041-210X.2010.00081.x.CrossRefGoogle Scholar
Šašić, L., Ačanski, J., Đan, M., Kočiš Tubić, N., Veličković, N., Radenković, S., and Vujić, A. 2018. Integrative taxonomy of Merodon caerulescens complex (Diptera: Syrphidae) – evidence of cryptic speciation. Matica Srpska Journal for Natural Sciences, 135: 103–118. https://doi.org/10.2298/ZMSPN1835103S.CrossRefGoogle Scholar
Šašić, L., Ačanski, J., Vujić, A., Ståhls, G., Radenković, S., Milić, D., et al. 2016. Molecular and morphological inference of three cryptic species within the Merodon aureus species group (Diptera: Syrphidae). Public Library of Science One, 11: e0160001. https://doi.org/10.1371/journal.pone.0160001.Google Scholar
Sheets, H.D. 2012. IMP software series. Canisius College, Buffalo, New York, United States of America.Google Scholar
Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H., and Flook, P. 1994. Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Annals of The Entomological Society of America, 87: 651–701. https://doi.org/10.1093/aesa/87.6.651.CrossRefGoogle Scholar
Speight, M.C.D. 2018. Species accounts of European Syrphidae, 2018. In Syrph the Net, the database of European Syrphidae (Diptera). Edited by M.C.D. Speight, E. Castella, J.-P. Sarthou, and C. Vanappelghem. Syrph the Net, 103: 1–302.Google Scholar
Ståhls, G., Vujić, A., Pérez-Bañón, C., Radenković, S., Rojo, S., and Petanidou, T. 2009. COI barcodes for identification of Merodon hoverflies (Diptera, Syrphidae) of Lesvos Island, Greece. Molecular Ecology Resources, 9: 1431–1438. https://doi.org/10.1111/j.1755-0998.2009.02592.x.CrossRefGoogle ScholarPubMed
Ståhls, G., Vujić, A., Petanidou, T., Cardoso, P., Radenković, S., Ačanski, J., et al. 2016. Phylogeographic patterns of Merodon hoverflies in the eastern Mediterranean region: revealing connections and barriers. Ecology and Evolution, 6: 2226–2245. https://doi.org/10.1002/ece3.2021.CrossRefGoogle ScholarPubMed
Stamatakis, A. 2014. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics, 30: 1312–1313. https://doi.org/10.1093/bioinformatics/btu033.CrossRefGoogle ScholarPubMed
Thompson, F.C. 1999. Key to the genera of the flower flies (Diptera: Syrphidae) of the Neotropical Region including descriptions of new genera and species and a glossary of taxonomic terms. Contribution on Entomology, International, 3: 321–378.Google Scholar
TIBCO Software. 2017. Statistica (data analysis software system), version 13. Available from www.statsoft.com/Products/STATISTICA-Features [accessed 16 October 2019].Google Scholar
Veselić, S., Vujić, A., and Radenković, S. 2017. Three new eastern-Mediterranean endemic species of the Merodon aureus group (Diptera: Syrphidae). Zootaxa, 4254: 401–434. http://doi.org/10.11646/zootaxa.4254.4.1.CrossRefGoogle Scholar
Vujić, A., Petanidou, T., Tscheulin, T., Cardoso, P., Radenković, S., Ståhls, G., et al. 2016. Biogeographical patterns of the genus Merodon Meigen, 1803 (Diptera: Syrphidae) in islands of the eastern Mediterranean and adjacent mainland. Insect Conservation and Diversity, 9: 181–191. https://doi.org/10.1111/icad.12156.CrossRefGoogle Scholar
Vujić, A., Radenković, S., Likov, L., Trifunov, S., and Nikolić, T. 2013. Three new species of the Merodon nigritarsis group (Diptera: Syrphidae) from the Middle East. Zootaxa, 3640: 442–464. http://doi.org/10.11646/zootaxa.3640.3.7.CrossRefGoogle ScholarPubMed
Vujić, A., Radenković, S., Ståhls, G., Ačanski, J., Stefanović, A., Veselić, S., et al. 2012. Systematics and taxonomy of the ruficornis group of genus Merodon Meigen (Diptera: Syrphidae). Systematic Entomology, 37: 578–602. https://doi.org/10.1111/j.1365-3113.2012.00631.x.CrossRefGoogle Scholar
Zelditch, M.L., Swiderski, D.L., Sheets, H.D., and Fink, W.L. 2004. Geometric morphometrics for biologists: a primer. Elsevier Academic Press, New York, New York, United States of America.Google Scholar
Supplementary material: File

Šašić Zorić et al. supplementary material

Table S2

Download Šašić Zorić et al. supplementary material(File)
File 9.5 KB
Supplementary material: File

Šašić Zorić et al. supplementary material

Table S1

Download Šašić Zorić et al. supplementary material(File)
File 13.4 KB