Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-24T12:04:56.875Z Has data issue: false hasContentIssue false
  • English
  • Français

A REFINED METHOD OF HANDLING ROOT MAGGOT LARVAE (DIPTERA: ANTHOMYIIDAE) FOR BIOASSAY1

Published online by Cambridge University Press:  31 May 2012

D. C. Read
D. C. Read
Affiliation:
Research Station, Canada Agriculture, Charlottetown, Prince Edward Island
Get access Rights & Permissions [Opens in a new window]

Abstract

A method is described for rearing and selecting strong, robust populations of first-stage root larvae for bioassay of insecticide toxicants in soil and host plant tissue. Oviposition dishes are placed in cages with flies for a 6-hour period 3 times a week. The eggs are then incubated on silk cloth over moist blotting paper in petri dishes at 21° to 22 °C until they hatch. When the larvae move away from the empty egg cases to a food supply, they are transferred to the centre of another piece of silk cloth lying fiat on a wet sponge. The strongest of the larvae move most quickly outward from the central mass and are transferred to holding trays and later set up for bioassay with plant tissue or treated soil. The weaker larvae are discarded. In 24- to 48-hour tests with larvae obtained by this method, there was no mortality in untreated samples. When larvae were reared to maturity, mortality in untreated media averaged approximately 6% in several hundred tests conducted from 1969 to 1971.

Type
Articles
Information
The Canadian Entomologist , Volume 104 , Issue 4 , April 1972 , pp. 597 - 601
Copyright
Copyright © Entomological Society of Canada 1972

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

de Vries, C. and Gijswijt, M. J.. 1968. A method for testing new insecticides under green-house conditions against the cabbage root fly (Delia brassicae (Bouché)-Diptera: Anthomyiidae). Meded. Rijksfak. Landb. 33: 927–935.Google Scholar
Harris, C. R. 1970. Laboratory evaluation of candidate materials as potential soil insecticides, III. J. econ. Ent. 63: 782–787.CrossRefGoogle Scholar
Harris, C. R. and Hitchon, J. L.. 1970. Laboratory evaluation of candidate materials as potential soil insecticides, II. J. econ. Ent. 63: 2–7.Google Scholar
Harris, C. R. and Mazurek, J. H.. 1966. Laboratory evaluation of candidate materials as potential soil insecticides. J. econ. Ent. 59: 1215–1221.CrossRefGoogle Scholar
Harris, C. R. and Svec, H. J.. 1966. Mass rearing of the cabbage maggot under controlled environmental conditions, with observations on the biology of cyclodiene-susceptible and resistant strains. J. econ. Ent. 59: 569–573.Google Scholar
Harris, C. R., Svec, H. J., and Begg, J. A.. 1966. Mass rearing of root maggots under controlled environmental conditions: seed-corn maggot, Hylemya cilicrura; bean seed fly, H. liturata; Euxesta notata; and Chaetopsis sp. J. econ. Ent. 59: 407–410.Google Scholar
Judge, F. D., Rinick, H. B., and MacEwen, F. L.. 1968. Field testing candidate insecticides on radish, cabbage and cauliflower for control of the cabbage in New York State. J. econ. Ent. 61: 1572–1577.CrossRefGoogle Scholar
MacLeod, D. G. R. 1964. Nutrition and feeding behavior of the adult onion maggot, Hylemya antiqua. J. econ. Ent. 57: 845–847.CrossRefGoogle Scholar
Matsumoto, Y. and Sugiyama, S.. 1967. A simple method for mass rearing of the seed-corn maggot, Hylemya platura Meigen. Ber. Ohara Inst. landw. Biol. 51: 221–225.Google Scholar
Matsumoto, Y. and Thorsteinson, A. J.. 1968. Olfactory response of larvae of the onion maggot, Hylemya antiqua Meigen (Diptera: Anthomyiidae) to organic sulfur compounds. Appl. Ent. Zool. 3: 107–111.Google Scholar
Niemczyk, H. D. 1964. Mass rearing of the onion maggot, Hylemya antiqua, under laboratory conditions. J. econ. Ent. 57: 57–60.Google Scholar
Read, D. C., Ayers, G. W., Campbell, J. E., and Robinson, D. B.. 1961. Note on an apparatus for maintaining constant soil temperatures and soil moistures, used in studies on soil inhibiting insects and diseases. Can. J. Pl. Sci. 41: 876–879.CrossRefGoogle Scholar
Read, D. C. 1965 a. Rearing root maggots, chiefly Hylemya brassicae (Bouché) (Diptera: Anthomyiidae) for bioassay. Can. Ent. 97: 136–141.Google Scholar
Read, D. C. 1965 b. Methods of testing Hylemya root maggots for insecticide resistance. J. econ. Ent. 58: 719–727.CrossRefGoogle Scholar
Read, D. C. 1969. Rearing the cabbage maggot with and without diapause. Can. Ent. 101: 725–737.CrossRefGoogle Scholar
Read, D. C. 1971 a. Bioactivity of Dasanit in a mineral soil, in rutabagas in the field and in storage, and effect of cooking on toxicants in the roots. J. econ. Ent. 64: 597–601.CrossRefGoogle Scholar
Read, D. C. 1971 b. Bioassays on the activation and deactivation of some new insecticides in a mineral soil and absorption of toxic components by rutabagas. J. econ. Ent. 64: 796–800.CrossRefGoogle Scholar