Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-22T18:04:40.075Z Has data issue: false hasContentIssue false

A Rapid Staining Method for Clinical Study of Cockroach Blood Cells1

Published online by Cambridge University Press:  31 May 2012

D. S. Sarkaria
Affiliation:
Department of Entomology, Cornell University, Ithaca, N.Y.
Sergio Bettini
Affiliation:
Department of Entomology, Cornell University, Ithaca, N.Y.
R. L. Patton
Affiliation:
Department of Entomology, Cornell University, Ithaca, N.Y.

Extract

In order to expedite the study of the effects of various biologically active chemicals upon the blood cells of insects a rapid clinical type method was needed that would allow preparations to be made with small (1 microliter or less) samples of blood which could be drawn without sacrificing the insect, and could be fixed and stained quickly for examination. A method meeting these requirements for the study of the blood cells of the American and German cockroaches has been developed.

Type
Articles
Copyright
Copyright © Entomological Society of Canada 1951

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Gatenby, J. B., and Beams, H. W.. Lee's Microtomist's Vade-Mecum. The Blakiston Company, Philadelphia, Pa., Toronto, Canada. 1950.Google Scholar
Jones, J. C.The Normal Hemocyte Picture of the Yellow Mealworm, Tenebrie molitor Linnaeus. Iowa State College Journal of Science 24: 355361. 1950.Google Scholar
Shull, W. E.Inhibition of Coagulation in the Blood of Insects by Fatty Acid Vapor Treatment. Ann. Ent. Soc. Amer. 29: 341349. 1936.CrossRefGoogle Scholar
Yeager, J. F., and Tauber, O.. On Counting Mitotically Dividing Cells in the Blood of the Cockroach, Periplaneta orientalis, Linn. Proc. Soc. Exp. Biol. and Med. 20: 861863. 1933.CrossRefGoogle Scholar
Yeager, J. F.A Modified Wright's Blood-staining Procedure for Smears of Heat-fixed Insect Blood. Ann. Ent. Soc. Amer. 31: 914. 1938.CrossRefGoogle Scholar
Yeager, J. F.Electrical Stimulation of Isolated Heart Preparations from Periplaneta americana. Jour. Agr. Res. 59: 121137. 1939.Google Scholar
Yeager, J. F.The Blood Picture of the Southern Armyworm (Prodenia eridania). Jour. Agr. Res. 71: 140. 1945.Google Scholar