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Prevalence of baculoviruses in spruce budworm (Lepidoptera: Tortricidae) populations in New Brunswick

Published online by Cambridge University Press:  02 April 2012

Christopher J. Lucarotti ,
Eldon S. Eveleigh ,
Tomo Royama ,
Benoit Morin ,
Peter McCarthy ,
Peter M. Ebling ,
William J. Kaupp ,
Claude Guertin  and
Max Arella
Christopher J. Lucarotti*
Affiliation:
Natural Resources Canada, Canadian Forest Service — Atlantic Forestry Centre, PO Box 4000, Fredericton, New Brunswick, Canada E3B 5P7
Eldon S. Eveleigh
Affiliation:
Natural Resources Canada, Canadian Forest Service — Atlantic Forestry Centre, PO Box 4000, Fredericton, New Brunswick, Canada E3B 5P7
Tomo Royama
Affiliation:
Natural Resources Canada, Canadian Forest Service — Atlantic Forestry Centre, PO Box 4000, Fredericton, New Brunswick, Canada E3B 5P7
Benoit Morin
Affiliation:
Natural Resources Canada, Canadian Forest Service — Atlantic Forestry Centre, PO Box 4000, Fredericton, New Brunswick, Canada E3B 5P7
Peter McCarthy
Affiliation:
Natural Resources Canada, Canadian Forest Service — Atlantic Forestry Centre, PO Box 4000, Fredericton, New Brunswick, Canada E3B 5P7
Peter M. Ebling
Affiliation:
Natural Resources Canada, Canadian Forest Service — Great Lakes Forestry Centre, 1219 Queen Street East, Sault Ste. Marie, Ontario, Canada P6A 2E5
William J. Kaupp
Affiliation:
Natural Resources Canada, Canadian Forest Service — Great Lakes Forestry Centre, 1219 Queen Street East, Sault Ste. Marie, Ontario, Canada P6A 2E5
Claude Guertin
Affiliation:
Institut Armand-Frappier, Centre de Recherche en Virologie, Laval, Quebec, Canada H7V 1B7
Max Arella
Affiliation:
Institut Armand-Frappier, Centre de Recherche en Virologie, Laval, Quebec, Canada H7V 1B7
*
1 Corresponding author (e-mail: [email protected]).
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Abstract

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Outbreak and declining populations of spruce budworm (Choristoneura fumiferana (Clem.)) were sampled extensively at three locations in New Brunswick, Canada, between 1982 and 1992 and were examined for the prevalence of granulosis and nuclear polyhedrosis viruses (Baculoviridae). Larvae, pupae, and adults were collected using a variety of methods. Spruce budworm nuclear polyhedrosis virus (CfMNPV) genomic DNA probes and wet-mount light microscopy were used to determine CfMNPV prevalence in 50 274 juvenile spruce budworms. Spruce budworm granulosis virus (ChfuGV) genomic DNA probes were used to determine the prevalence of ChfuGV in 25 703 of these same samples. The prevalence of both viruses was low, with ChfuGV and CfMNPV not found in more than 15% and 2%, respectively, of samples in any collection in a given year. Prevalence of ChfuGV was greatest in mid- to late June in sixth-instar larvae. Each virus was detected in only two of 2177 female moths and in none of the 420 male moths examined. In the entire collection, cytoplasmic polyhedrosis virus (Reoviridae) was detected in only two budworm larvae and entomopoxvirus (Poxviridae) was not detected in any.

Résumé

Des populations épidémiques et en déclin de la tordeuse des bourgeons de l'épinette (TBE) (Choristoneura fumiferana (Clem.)) situées à trois endroits au Nouveau Brunswick, Canada, ont été échantillonnées à grande échelle entre 1982 et 1992 et ont été examinées pour évaluer la prévalence des virus de la granulose et de la polyhedrose nucléaire (Baculoviridae). Les larves, les chrysalides et les adultes ont été récoltés par une variété de méthodes. Des sondes d'ADN génomique du virus de la polyhedrose nucléaire de la TBE (CfMNPV) ainsi que des frottis d'insectes ont été utilizés pour déterminer la prévalence du CfMNPV chez 50 274 individus juvéniles de la TBE. Des sondes d'ADN génomique du virus de la granulose de la TBE (ChfuGV) ont été utilisées pour déterminer sa prévalence chez 25 703 de ces mêmes échantillons. La prévalence des deux virus était faible, ne dépassant pas 15% pour ChfuGV et pas plus de 2% pour le CfMNPV, pour chaque méthode d'échantillonage, pour chaque année d'étude. La prévalence du ChfuGV atteint son maximum entre le milieu et la fin de juin, au sixième stade de développement larvaire. Chaque virus n'a été détecté que chez deux des 2177 adultes femelles et chez aucun des 420 adultes mâles examinés. Parmis tous les specimens recueillis, le virus de la polyhèdrose cytoplasmique (Reoviridae) n'a été détecté que dans deux larves de la TBE alors que l'entomopoxvirus (Poxviridae) ne l'a été dans aucun.

Type
Articles
Information
The Canadian Entomologist , Volume 136 , Issue 2 , April 2004 , pp. 255 - 264
Copyright
Copyright © Entomological Society of Canada 2004

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