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Prevalence of baculoviruses in spruce budworm (Lepidoptera: Tortricidae) populations in New Brunswick

Published online by Cambridge University Press:  02 April 2012

Christopher J. Lucarotti ,
Eldon S. Eveleigh ,
Tomo Royama ,
Benoit Morin ,
Peter McCarthy ,
Peter M. Ebling ,
William J. Kaupp ,
Claude Guertin  and
Max Arella
Christopher J. Lucarotti*
Affiliation:
Natural Resources Canada, Canadian Forest Service — Atlantic Forestry Centre, PO Box 4000, Fredericton, New Brunswick, Canada E3B 5P7
Eldon S. Eveleigh
Affiliation:
Natural Resources Canada, Canadian Forest Service — Atlantic Forestry Centre, PO Box 4000, Fredericton, New Brunswick, Canada E3B 5P7
Tomo Royama
Affiliation:
Natural Resources Canada, Canadian Forest Service — Atlantic Forestry Centre, PO Box 4000, Fredericton, New Brunswick, Canada E3B 5P7
Benoit Morin
Affiliation:
Natural Resources Canada, Canadian Forest Service — Atlantic Forestry Centre, PO Box 4000, Fredericton, New Brunswick, Canada E3B 5P7
Peter McCarthy
Affiliation:
Natural Resources Canada, Canadian Forest Service — Atlantic Forestry Centre, PO Box 4000, Fredericton, New Brunswick, Canada E3B 5P7
Peter M. Ebling
Affiliation:
Natural Resources Canada, Canadian Forest Service — Great Lakes Forestry Centre, 1219 Queen Street East, Sault Ste. Marie, Ontario, Canada P6A 2E5
William J. Kaupp
Affiliation:
Natural Resources Canada, Canadian Forest Service — Great Lakes Forestry Centre, 1219 Queen Street East, Sault Ste. Marie, Ontario, Canada P6A 2E5
Claude Guertin
Affiliation:
Institut Armand-Frappier, Centre de Recherche en Virologie, Laval, Quebec, Canada H7V 1B7
Max Arella
Affiliation:
Institut Armand-Frappier, Centre de Recherche en Virologie, Laval, Quebec, Canada H7V 1B7
*
1 Corresponding author (e-mail: [email protected]).
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Abstract

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Outbreak and declining populations of spruce budworm (Choristoneura fumiferana (Clem.)) were sampled extensively at three locations in New Brunswick, Canada, between 1982 and 1992 and were examined for the prevalence of granulosis and nuclear polyhedrosis viruses (Baculoviridae). Larvae, pupae, and adults were collected using a variety of methods. Spruce budworm nuclear polyhedrosis virus (CfMNPV) genomic DNA probes and wet-mount light microscopy were used to determine CfMNPV prevalence in 50 274 juvenile spruce budworms. Spruce budworm granulosis virus (ChfuGV) genomic DNA probes were used to determine the prevalence of ChfuGV in 25 703 of these same samples. The prevalence of both viruses was low, with ChfuGV and CfMNPV not found in more than 15% and 2%, respectively, of samples in any collection in a given year. Prevalence of ChfuGV was greatest in mid- to late June in sixth-instar larvae. Each virus was detected in only two of 2177 female moths and in none of the 420 male moths examined. In the entire collection, cytoplasmic polyhedrosis virus (Reoviridae) was detected in only two budworm larvae and entomopoxvirus (Poxviridae) was not detected in any.

Résumé

Des populations épidémiques et en déclin de la tordeuse des bourgeons de l'épinette (TBE) (Choristoneura fumiferana (Clem.)) situées à trois endroits au Nouveau Brunswick, Canada, ont été échantillonnées à grande échelle entre 1982 et 1992 et ont été examinées pour évaluer la prévalence des virus de la granulose et de la polyhedrose nucléaire (Baculoviridae). Les larves, les chrysalides et les adultes ont été récoltés par une variété de méthodes. Des sondes d'ADN génomique du virus de la polyhedrose nucléaire de la TBE (CfMNPV) ainsi que des frottis d'insectes ont été utilizés pour déterminer la prévalence du CfMNPV chez 50 274 individus juvéniles de la TBE. Des sondes d'ADN génomique du virus de la granulose de la TBE (ChfuGV) ont été utilisées pour déterminer sa prévalence chez 25 703 de ces mêmes échantillons. La prévalence des deux virus était faible, ne dépassant pas 15% pour ChfuGV et pas plus de 2% pour le CfMNPV, pour chaque méthode d'échantillonage, pour chaque année d'étude. La prévalence du ChfuGV atteint son maximum entre le milieu et la fin de juin, au sixième stade de développement larvaire. Chaque virus n'a été détecté que chez deux des 2177 adultes femelles et chez aucun des 420 adultes mâles examinés. Parmis tous les specimens recueillis, le virus de la polyhèdrose cytoplasmique (Reoviridae) n'a été détecté que dans deux larves de la TBE alors que l'entomopoxvirus (Poxviridae) ne l'a été dans aucun.

Type
Articles
Information
The Canadian Entomologist , Volume 136 , Issue 2 , April 2004 , pp. 255 - 264
Copyright
Copyright © Entomological Society of Canada 2004

References

Barrett, J.W., Ladd, T.R., Primavera, M.J., Retnakaran, A., Sohi, S.S., Palli, S.R. 2000. Nucleopolyhedrovirus pathology in spruce budworm larvae. The Canadian Entomologist 132: 581–90Google Scholar
Cunningham, J.C., Kaupp, W.J. 1995. Insect viruses. pp 327–40 in Armstrong, J.A., Ives, W.G.H. (Eds), Forest insect pests in Canada. Ottawa, Ontario: Natural Resources CanadaGoogle Scholar
Doane, C.C. 1975. Infectious sources of nuclear polyhedrosis virus persisting in natural habitats of the gypsy moth. Environmental Entomology 4: 392–4Google Scholar
Ebling, P.M., Barrett, J.W., Arif, B.M. 1998. Pathogenicity of the Ireland strain of nuclear polyhedrosis virus to spruce budworm, Choristoneura fumiferana larvae. The Canadian Entomologist 130: 107–8CrossRefGoogle Scholar
Forté, A.J., Guertin, C., Cabana, J. 1999. Pathogenicity of a granulovirus towards Choristoneura fumiferana (Lepidoptera: Tortricidae). The Canadian Entomologist 131: 725–7Google Scholar
Greenbank, D.O. 1963. Host species and the spruce budworm. Memoirs of the Entomological Society of Canada 31: 219–23Google Scholar
Kaupp, W.J., Ebling, P.M. 1990. Response of third-, fourth-, fifth-, and sixth-instar spruce budworm, Choristoneura fumiferana (Clem.), larvae to nuclear polyhedrosis virus. The Canadian Entomologist 122: 1037–8Google Scholar
Kaupp, W.J., Ebling, P.M. 1993. Horseradish peroxidase-labelled probes and enhanced chemiluminescence to detect baculoviruses in gypsy moth and eastern spruce budworm larvae. Journal of Virological Methods 44: 89–98Google Scholar
Kaupp, W.J., Cunningham, J.C., Cadogan, B.L. 1990. Aerial application of high dosages of nuclear polyhedrosis virus to early instar spruce budworm, Choristoneura fumiferana (Clem.). Information report FPMI-X- 85. Sault Ste. Marie, Ontario: Forestry Canada, Forest Pest Management InstituteGoogle Scholar
Kukan, B. 1999. Vertical transmission of nucleopolyhedrovirus in insects. Journal of Invertebrate Pathology 74: 103–11CrossRefGoogle ScholarPubMed
Lethiecq, J-L, Régnière, J. 1988. Comparative description of the physical characteristics and vegetation of six sites used by the Canadian Forestry Service in the study of the spruce budworm population dynamics. Canadian Forestry Service, Laurentian Forestry Centre, Information Report LAU-X-83Google Scholar
Loughton, B.G., Derry, C., West, A.S. 1963. Spiders and the spruce budworm. Memoirs of the Entomological Society of Canada 31: 249–68CrossRefGoogle Scholar
Lucarotti, C.J. 1997. Baculoviruses as management tools in the control of spruce budworm and other forest pest insects. pp 7–8in Régnière, J., Delisle, J. (Eds), Proceedings of the 17th Eastern Spruce Budworm Work Conferences, Québec, Quèbec, 2–3 April 1997. Canadian Forest Service, Laurentian Forestry Centre, Information Report LAU-X-113BGoogle Scholar
McGugan, B.M. 1954. Needle-mining habits and larval instars of the spruce budworm. The Canadian Entomologist 86: 439–54Google Scholar
McMorran, A. 1965. A synthetic diet for the spruce budworm, Choristoneura fumiferana (Clem.) (Lepidoptera: Tortricidae). The Canadian Entomologist 97: 58–62CrossRefGoogle Scholar
Miller, C.A. 1963 a. The spruce budworm. Memoirs of the Entomological Society of Canada 31: 12–19CrossRefGoogle Scholar
Miller, C.A. 1963 b. Parasites and the spruce budworm. Memoirs of the Entomological Society of Canada 31: 228–44CrossRefGoogle Scholar
Mook, L.J. 1963. Birds and the spruce budworm. Memoirs of the Entomological Society of Canada 31: 268–71Google Scholar
Morris, R.F. 1963 a. Introduction. Memoirs of the Entomological Society of Canada 31: 7–12CrossRefGoogle Scholar
Morris, R.F. 1963 b. Predation and the spruce budworm. Memoirs of the Entomological Society of Canada 31: 244–8CrossRefGoogle Scholar
Neilson, M.M. 1963. Disease and the spruce budworm. Memoirs of the Entomological Society of Canada 31: 272–87Google Scholar
Otvos, I.S., Cunningham, J.C., Shepherd, R.F. 1995. Douglas-fir tussock moth, Orgyia pseudotsugata. pp 127–132in Armstrong, J.A., Ives, W.G.H. (Eds), Forest insect pests in Canada. Ottawa, Ontario: Natural Resources CanadaGoogle Scholar
Podgwaite, J.D., Shields, K.S., Zerillo, R.T., Bruen, R.B. 1979. Environmental persistence of the nucleopolyhedrosis virus of the gypsy moth, Lymantria dispar. Environmental Entomology 8: 528–36CrossRefGoogle Scholar
Retnakaran, A., Tomkins, W.L., Primavera, M.J., Palli, S.R. 1999. Feeding behaviour of the first-instar Choristoneura fumiferana and Choristoneura pinus pinus (Lepidoptera: Torticidae). The Canadian Entomologist 131: 79–84CrossRefGoogle Scholar
Royama, T. 1984. Population dynamics of the spruce budworm Choristoneura fumiferana. Ecological Monographs 54: 429–62CrossRefGoogle Scholar
Royama, T. 1992. Analytical population dynamics. London: Chapman and HallCrossRefGoogle Scholar
Royama, T. 2001. Measurement, analysis, and interpretation of mortality factors in insect survivorship studies, with reference to the spruce budworm, Choristoneura fumiferana (Clem.) (Lepidoptera: Tortricidae). Population Ecology 43: 157–78CrossRefGoogle Scholar
Strongman, D.B., Eveleigh, E.S., van Frankenhuyzen, K., Royama, T. 1997. The occurrence of two types of entomopathogenic bacilli in natural populations of the spruce budworm, Choristoneura fumiferana. Canadian Journal of Forest Research 27: 1922–7Google Scholar
Weseloh, R.M., Andreadis, T.G. 1992. Epizootiology of the fungus, Entomophaga maimaiga, and its impact on gypsy moth populations. Journal of Invertebrate Pathology 59: 133–41Google Scholar
West, R.J., Cunninghan, J.C., Kaupp, W.J. 1987. Ground spray applications of Virtuss®, a nuclear polyhedrosis virus, against whitemarked tussock moth larvae at Bottom Brook, Newfoundland in 1986. Information report N-X-257. St. John's, Newfoundland: Forestry Canada, Newfoundland and Labrador RegionGoogle Scholar
West, R.J., Kaupp, W.J., Cunningham, J.C. 1989. Aerial applications of Virtuss®, a nuclear polyhedrosis virus, against whitemarked tussock moth in Newfoundland in 1987. Information report N-X-270. St. John's, Newfoundland: Forestry Canada, Newfoundland and Labrador RegionGoogle Scholar
Woods, S.A., Elkinton, J.S. 1987. Bimodal patterns of mortality from nuclear polyhedrosis virus in gypsy moth (Lymantria dispar) populations. Journal of Invertebrate Patholology 50: 151–7Google Scholar
Yuen, L., Dionne, J., Arif, B., Richardson, C. 1990. Identification and sequencing of the spheroidin gene of Choristoneura fumiferana entomopoxvirus. Virology 182: 427–33Google Scholar