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Population structure and phylogenetic relationships of Ceutorhynchus neglectus (Coleoptera: Curculionidae)

Published online by Cambridge University Press:  02 April 2012

R. D. Laffin ,
L. M. Dosdall  and
F.A.H. Sperling
R. D. Laffin*
Affiliation:
Department of Biological Sciences, ESB 2-08 Earth Sciences Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2E9
L. M. Dosdall
Affiliation:
Department of Agricultural, Food and Nutritional Science, 4-10 Agriculture/Forestry Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2P5
F.A.H. Sperling
Affiliation:
Department of Biological Sciences, ESB 2-08 Earth Sciences Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2E9
*
1 Corresponding author (e-mail: [email protected]).
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Abstract

Ceutorhynchus neglectus Blatchley is a weevil that is native to, and widely distributed in, North America. It has life-history characteristics similar to its alien invasive congener, Ceutorhynchus obstrictus (Marsham), the cabbage seedpod weevil. Our study was undertaken to compare the population structure of C. neglectus in North America to that of C. obstrictus, which, in contrast, was introduced only recently to North America and might be expected to have a simpler population structure. We also compared the population structure of C. neglectus to that of Pissodes strobi (Peck), which is known to possess high levels of intraspecific variation and is also a Nearctic weevil. We sequenced a 790-bp fragment of mtDNA (cytochrome oxidase I (COI) gene) and a 117-bp fragment of nuclear DNA (internal transcribed spacer region 1 (ITS1)). Nested clade analysis inferred contiguous range expansion and restricted gene flow with isolation by distance. Analysis of molecular variance also supported restricted gene flow between geographically distant populations. However, within-species variation in C. neglectus was lower than that for other weevil species including C. obstrictus. We also examined DNA divergences and phylogenetic relationships among 10 species of Ceutorhynchus using parsimony analysis of a 2.3-kb fragment of mtDNA (COI–COII) and a 541-bp fragment of nuclear DNA (elongation factor 1α).

Résumé

Ceutorhynchus neglectus Blatchley est un charançon indigène à l'Amérique du Nord où il a une grande répartition géographique. Les caractéristiques de son cycle biologique ressemblent à celles de son congénère exotique et envahissant, Ceutorhynchus obstrictus (Marsham), le charançon de la silique. Notre étude veut comparer la structure de population de C. neglectus en Amérique du Nord à celle de C. obstrictus qui doit vraisemblablement avoir une structure de population plus simple, car il n'a été introduit que récemment en Amérique du Nord. Nous avons aussi comparé la structure de population de C. neglectus à celle de Pissodes strobi (Peck) qui est bien caractérisé par une forte variation intraspécifique et qui est aussi une espèce néarctique. Nous avons séquencé un fragment de 790 pb d'ADNmt (le gène de la cytochrome C oxydase, COI) et un fragment de 117 pb d'ADN nucléaire (l'espaceur interne transcrit 1, ITS1). Une analyse cladistique emboîtée indique une expansion de l'aire de répartition par contiguïté et un flux génique restreint par l'isolement relié à la distance. Une analyse AMOVA confirme aussi le flux génique restreint entre les populations éloignées géographiquement. Cependant, la variation intraspécifique chez C. neglectus est plus faible que chez les autres charançons, y compris C. obstrictus. Nous avons aussi examiné les divergences de l'ADN et les relations phylogénétiques chez 10 espèces de Ceutorhynchus par une analyse de parcimonie d'un fragment de 2,3 kb d'ADNmt (COI–COII) et d'un fragment de 541 pb d'ADN nucléaire (le facteur d'élongation 1α).

[Traduit par la Rédaction]

Type
Articles
Information
The Canadian Entomologist , Volume 137 , Issue 6 , December 2005 , pp. 672 - 684
Copyright
Copyright © Entomological Society of Canada 2005

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