Hostname: page-component-586b7cd67f-gb8f7 Total loading time: 0 Render date: 2024-11-28T10:40:36.519Z Has data issue: false hasContentIssue false
  • English
  • Français

PATHOGENICITY OF THE IRELAND STRAIN OF NUCLEAR POLYHEDROSIS VIRUS TO SPRUCE BUDWORM, CHORISTONEURA FUMIFERANA, LARVAE

Published online by Cambridge University Press:  31 May 2012

P.M. Ebling ,
J.W. Barrett  and
B.M. Arif
P.M. Ebling
Affiliation:
Canadian Forest Service, Natural Resources Canada, P.O. Box 490, Sault Ste. Marie, Ontario, Canada P6A 5M7
J.W. Barrett
Affiliation:
Canadian Forest Service, Natural Resources Canada, P.O. Box 490, Sault Ste. Marie, Ontario, Canada P6A 5M7
B.M. Arif
Affiliation:
Canadian Forest Service, Natural Resources Canada, P.O. Box 490, Sault Ste. Marie, Ontario, Canada P6A 5M7
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The spruce budworm, Choristoneura fumiferana (Clem.), nuclear polyhedrosis virus (CfMNPV) has been studied extensively for its potential use as a bio-insecticide (Cunningham 1995). Recent advances in recombinant DNA technology have been impetuous in the genetic engineering of this virus to increase its virulence and (or) speed of action. This aspect of research has been concentrated on the modification of a plaque purified (Ireland strain) isolate obtained from the wild-type virus population (Arif et al. 1984). To assess any improvement in the effectiveness against the target pest, the virulence of the unaltered virus must first be determined. Bioassay results that have been previously reported by Kaupp and Ebling (1990) were conducted on the wild-type virus found in nature which consists of a mixture of viruses (Arif et al. 1994). Results of bioassays conducted to determine dose–response and time–response of fifth-instar spruce budworm larvae to the Ireland strain of CfMNPV are reported here.

Type
Articles
Information
The Canadian Entomologist , Volume 130 , Issue 1 , February 1998 , pp. 107 - 108
Copyright
Copyright © Entomological Society of Canada 1998

References

Arif, B.M., and Brown, K.W.. 1975. Purification and properties of a nuclear polyhedrosis virus from Choristoneura fumiferana. The Canadian Journal of Microbiology 21: 1224–1231.CrossRefGoogle ScholarPubMed
Arif, B.M., Jamieson, P., Sohi, S.S., Kaupp, W., and MacDonald, J.A.. 1994. Symbiotic-like relationship between two MNPV's of the spruce budworm, Choristoneura fumiferana. pp. 145–146in Abstracts, Vol. II, VIth International Colloquium on Invertebrate Pathology and Microbial Control, Montpellier, France. Society for Invertebrate Pathology.Google Scholar
Arif, B.M., Kuzio, J., Faulkner, P., and Doerfler, W.. 1984. The genome of Choristoneura fumiferana nuclear polyhedrosis virus: molecular cloning and mapping of the EcoRI, BamHI, SmaI, XbaI and BglII restriction sites. Virus Research 1: 605–614.Google Scholar
Cunningham, J.C. 1995. Baculoviruses as microbial insecticides. pp. 261–292in Reuveni, R. (Ed.), Novel Approaches to Integrated Pest Management. CRC Press, Boca Raton, Florida.Google Scholar
Ebling, P.M., and Kaupp, W.J.. 1997. Pathogenicity of a nuclear polyhedrosis virus to forest tent caterpillar, Malacosoma disstria (Hübner) (Lepidoptera: Lasiocampidae). The Canadian Entomologist 129: 195–196.Google Scholar
Kaupp, W.J., and Ebling, P.M.. 1990. Response of third-, fourth-, fifth-, and sixth-instar spruce budworm, Choristoneura fumiferana (Clem.), larvae to nuclear polyhedrosis virus. The Canadian Entomologist 122: 1037–1038.Google Scholar
LeOra Software. 1994. POLO-PC: a user's guide to Probit Or LOgit analysis. LeOra Software, 1119 Shattuck Ave., Berkeley, California.Google Scholar
McMorran, A. 1965. A synthetic diet of the spruce budworm, Choristoneura fumiferana (Clem.) (Lepidoptera: Tortricidae). The Canadian Entomologist 97: 58–82.CrossRefGoogle Scholar
Robertson, J.L., and Preisler, H.K.. 1992. Pesticide bioassays with arthropods. CRC Press, London.Google Scholar
Wigley, P.J. 1980. Counting micro-organisms. pp. 29–35in Kalmakoff, J., and Longworth, J.F. (Eds.), Microbial Control of Insect Pests. New Zealand Department of Scientific and Industrial Research Bulletin 228.Google Scholar