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Nesting biology and DNA barcode analysis of Ceratina dupla and C. mikmaqi, and comparisons with C. calcarata (Hymenoptera: Apidae: Xylocopinae)

Published online by Cambridge University Press:  03 January 2012

J.L. Vickruck ,
S.M. Rehan ,
C.S. Sheffield  and
M.H. Richards
J.L. Vickruck*
Affiliation:
Department of Biological Sciences, Brock University, 500 Glenridge Avenue, St. Catharines, Ontario, Canada L2S 3A1
S.M. Rehan
Affiliation:
Department of Biological Sciences, Brock University, 500 Glenridge Avenue, St. Catharines, Ontario, Canada L2S 3A1
C.S. Sheffield
Affiliation:
Department of Biology, York University, 4700 Keele Street, Toronto, Ontario, Canada M3J 1P3
M.H. Richards
Affiliation:
Department of Biological Sciences, Brock University, 500 Glenridge Avenue, St. Catharines, Ontario, Canada L2S 3A1
*
1Corresponding author (e-mail: [email protected]).
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Abstract

Using DNA barcode analysis, nest collections, and pan-trapping we compared molecular differences, nesting behaviour, and phenology of three of the four species of Ceratina Latreille present in the Niagara Region of southern Ontario, Canada: C. dupla Say, C. calcarata Robertson, and C. mikmaqi Rehan and Sheffield. Ceratina dupla and C. mikmaqi were separated by five fixed nucleotide differences and an average sequence divergence of 1.86%. In our population, C. mikmaqi and C. calcarata were common and C. dupla was rare. Ceratina dupla nested earlier than C. mikmaqi and C. calcarata, and sometimes produced a second brood in late July – early August. Each species constructed linear nests in the pith of dead twigs, C. mikmaqi and C. dupla usually in Fuller's teasel (Dipsacus fullonum L.; Dipsacaceae) and C. calcarata usually in raspberry (Rubus L.; Rosaceae). Genetically distinct, each species occupies a slightly different niche in the Niagara bee assemblage.

Résumé

À l'aide d'une analyse des codes de barre d'ADN, de récoltes de nids et de piégeage avec des plateaux, nous comparons les différences moléculaires, le comportement de nidification et la phénologie de trois des quatre espèces de Ceratina Latreille présentes dans la région de Niagara du sud de l'Ontario, Canada, soit C. dupla Say, C. calcarata Robertson et C. mikmaqi Rehan et Sheffield. Ceratina dupla et C. mikmaqi se distinguent par des différences fixes dans cinq nucléotides et la divergence moyenne de leurs séquences est de 1,86 %. Dans notre peuplement, C. mikmaqi et C. calcarata sont communs, alors que C. dupla est rare. Ceratina dupla niche plus tôt que C. mikmaqi et C. calcarata et produit quelquefois une seconde portée à la fin de juillet — début d'août. Chaque espèce construit un nid linéaire dans la moelle de brindilles mortes, C. mikmaqi et C. dupla généralement de cardère des foulons (Dipsacus fullonum L.; Dipsacaceae) et C. calcarata de framboisiers (Rubus L.; Rosaceae). Chacune des espèces, génétiquement distincte, occupe une niche légèrement différente au sein du peuplement d'abeilles de la région de Niagara.

[Traduit par la Rédaction]

Type
Biodiversity & Evolution
Information
The Canadian Entomologist , Volume 143 , Issue 3 , June 2011 , pp. 254 - 262
Copyright
Copyright © Entomological Society of Canada 2011

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