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Morphology of testes, spermatogenesis, sperm bundles, and spermatozoa of Kerria chinensis (Hemiptera: Kerriidae)

Published online by Cambridge University Press:  19 September 2018

Lucksanaveejit Seubparu ,
Mingkwan Nipitwathanaphon ,
Wijit Wisoram ,
David Merritt  and
Lertluk Ngernsiri
Lucksanaveejit Seubparu
Affiliation:
Department of Genetics, Faculty of Science, Kasetsart University, 50 Ngamwongwan Road, Lat Yao, Chatuchak, Bangkok, 10900, Thailand
Mingkwan Nipitwathanaphon
Affiliation:
Centre of Advanced Studied in Tropical Natural Resources, Kasetsart University, 50 Ngamwongwan Road, Lat Yao, Chatuchak, Bangkok 10900, Thailand
Wijit Wisoram
Affiliation:
Faculty of Science and Technology, Rajamangala University of Technology Tawan-ok, 43 Moo6 Bangpra, Sriracha, Chonburi, 20110, Thailand
David Merritt
Affiliation:
School of Biological Sciences, The University of Queensland, Brisbane, QLD 4072, Australia
Lertluk Ngernsiri*
Affiliation:
Centre of Advanced Studied in Tropical Natural Resources, Kasetsart University, 50 Ngamwongwan Road, Lat Yao, Chatuchak, Bangkok 10900, Thailand
*
1Corresponding author (e-mail: [email protected])
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Abstract

The filamentous spermatozoa of scale insects (Hemiptera) are highly modified compared with those of typical insects. Here, we investigate the morphology of the testes, sperm bundles, spermatozoa, and spermatogenesis of the wingless Kerria chinensis (Mahdihassan) (Hemiptera: Kerriidae), a shellac-producing scale insect. Each testis contains two antiparallel groups of several hundred syncytial sperm bundles. In each spermatocyte cyst, 16 primary spermatocytes divide via inverted meiosis, resulting in 16 quadrinucleated spermatids, each having two euchromatic and two heterochromatic nuclei. During spermiogenesis, each spermatid produces two spermatozoa protruding out of the spermatid close to the two euchromatic nuclei and their tails then grow in opposite directions. In each cyst, the 32 spermatozoa form two sperm bundles lying in an antiparallel direction oriented to different ends of the testis. Each spermatozoon has three distinct regions, an apex, a filamentous region and a tail. The spermatozoa have long thread-like nuclear cores that occupy about one-fourth of the sperm body length, located primarily in the posterior half. At the anterior end of the spermatozoon is a translucent, swollen vesicle and a distal, densely-stained structure; a putative acrosome of a type not previously reported in the spermatozoa of scale insects.

Type
Physiology, Biochemisty, Development, & Genetics
Information
The Canadian Entomologist , Volume 150 , Issue 5 , October 2018 , pp. 594 - 609
Copyright
© Entomological Society of Canada 2018 

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