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LABORATORY BIOASSAYS OF MIXTURES OF BACILLUS THURINGIENSIS AND CHITINASE1

Published online by Cambridge University Press:  31 May 2012

Paul G. Fast
Paul G. Fast
Affiliation:
Insect Pathology Research Institute, Canadian Forestry Service, Sault Ste. Marie, Ontario P6A 5M7
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Abstract

The addition of chitinase at the rate of 1000–4000 nephelometric units/U.S. gallon to Bacillus thuringiensis insecticide for use against spruce budworm has been advocated.

Bioassays of V instar spruce budworm larvae against seven levels of Thuricide® at four levels of chitinase corresponding to 0, 22, 220, and 2200 nephelometric units/U.S. gallon were conducted by placing larvae on balsam fir foliage (Abies balsamea) dipped in appropriate dilutions of Thuricide® with chitinase. Mortality was assessed 6 days later. An additional replicated assay at a relatively massive dose of chitinase, 33,000 nephelometric units/U.S. gallon, was compared simultaneously with a 0 chitinase control. The data were homogeneous and linear by statistical standards. No effect of chitinase on LC50 or slope of the regression line was observed.

Type
Articles
Information
The Canadian Entomologist , Volume 110 , Issue 2 , 01 February 1978 , pp. 201 - 203
Copyright
Copyright © Entomological Society of Canada 1978

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References

Berger, L. R. and Reynolds, D. M.. 1958. The chitinase system of a strain of Streptomyces griseus. Biochim. Biophys. Acta 29: 522–534.Google Scholar
Dimond, J. B.1972. A demonstration of Bacillus thuringiensis plus the enzyme chitinase against the spruce budworm in Maine. Part I Efficacy. Misc. Rep. Maine agric. Exp. Stn 144.Google Scholar
Dimond, J. B.1975. Efficacy of materials tested for control of spruce budworm damage in Maine. I. Chemical insecticides. II. Biological insecticides. Misc. Rep. Maine Life Sci. and Agric. Exp. Stn 174.Google Scholar
Fast, P. G.1977. Bacillus thuringiensis δ-endotoxin: On the relative roles of spores and crystals in toxicity to spruce budworm (Lepidoptera: Tortricidae). Can. Ent. 110: 1515–1518.Google Scholar
Finney, D. J.1971. Probit analysis, 3rd ed. Cambridge University Press. 333 pp.Google Scholar
Grisdale, D.1970. An improved laboratory method for rearing large numbers of spruce budworm, Choristoneura fumiferana (Lepidoptera: Tortricidae). Can. Ent. 102: 1111–1117.Google Scholar
Monreal, J. and Reese, E. T.. 1969. The chitinase of Serratia marcescens. Can. J. Microbiol. 15: 689–696.Google Scholar
Morris, O. N.1973. Dosage-mortality studies with commercial Bacillus thuringiensis sprayed in a modified Potters Tower against some forest insects. J. invert. Path. 22: 108–114.Google Scholar
Morris, O. N.1976. A 2-year study of the efficacy of Bacillus thuringiensis – chitinase combinations in spruce budworm (Choristoneura fumiferana) control. Can. Ent. 108: 225–233.Google Scholar
Nigam, P. C. 1974. Laboratory study of comparative toxicity against fifth instar larvae. In Bacillus thuringiensis: Evaluation of commercial preparations of Bacillus thuringiensis with and without chitinase against spruce budworm. Inf. Rep. Chem. Control Res. Inst. CC-X-59.Google Scholar
Smirnoff, W. A.1971. Effect of chitinase on the action of Bacillus thuringiensis. Can. Ent. 103: 1829–1831.Google Scholar
Smirnoff, W. A. 1973 a. The possible use of Bacillus thuringiensis plus chitinase formulation for the control of spruce budworm outbreaks. Jl N.Y. Acad. Sci. 81: 196–200.Google Scholar
Smirnoff, W. A. 1973 b. Results of tests with Bacillus thuringiensis and chitinase on larvae of the spruce budworm. J. invert. Path. 21: 116–118.CrossRefGoogle Scholar
Smirnoff, W. A. 1976. Analysis of operational application of Bacillus thuringiensis against spruce budworm. Proc. N. Am. Symp. on Microbial Control of Spruce Budworm. Abbott Laboratories Ltd., Montreal.Google Scholar
Smirnoff, W. A. 1977. Confirmations expérimentales du potential du complexe Bacillus thuringiensis et chitinase pour la répression de la tordeuse des bourgeons de l'épinette, Choristoneura fumiferana (Lepidoptera: Tortricidae). Can. Ent. 109: 351–358.CrossRefGoogle Scholar
Smirnoff, W. A., Randall, A. P., Martineau, R., Haliburton, W., and Juneau, A.. 1973. Field test of the effectiveness of chitinase additive to Bacillus thuringienses Berliner against Choristoneura fumiferana (Clem.). Can. J. For. Res. 3: 228–236.CrossRefGoogle Scholar