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INDUCTION OF PHEROMONE PRODUCTION IN FEMALES OF HELIOTHIS VIRESCENS (F.) AND H. SUBFLEXA (GN.) (LEPIDOPTERA: NOCTUIDAE) DURING THE PHOTOPHASE

Published online by Cambridge University Press:  31 May 2012

P.E.A. Teal ,
A. Oostendorp  and
J.H. Tumlinson
P.E.A. Teal
Affiliation:
Insect Attractants, Behavior and Basic Biology Research Laboratory, USDA, ARS, PO Box 14565, Gainesville, Florida, USA 32604
A. Oostendorp
Affiliation:
Insect Attractants, Behavior and Basic Biology Research Laboratory, USDA, ARS, PO Box 14565, Gainesville, Florida, USA 32604
J.H. Tumlinson
Affiliation:
Insect Attractants, Behavior and Basic Biology Research Laboratory, USDA, ARS, PO Box 14565, Gainesville, Florida, USA 32604
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Abstract

Production of sex pheromone was induced during the photophase, a time during which pheromone is not normally produced, in females of Heliothis virescens (F.) and H. subflexa (Gn.) by injection of homogenates and partially purified extracts of the brain – subesophageal ganglion complex of conspecific females or H. zea (Boddie) females or synthetic pheromone biosynthesis activating neuropeptide (PBAN). The amount of (Z)-11-hexadecenal, the major component of the pheromone of both species, increased during the first 60 min after injection of PBAN then leveled off and declined in H. virescens. In H. subflexa, the amount of this aldehyde increased during the first 120 min and declined after 240 min. Studies indicated that extracts of the brain – subesophageal ganglion complex that did not contain neurally produced biogenic amines induced production of as much pheromone as did homogenates containing these compounds. Dose-response studies indicated that the optimal dose of synthetic PBAN for induction of pheromone during a 60-min incubation was 5.0 pmol. In vivo maintenance of pheromone production required continuous stimulation of the pheromone gland.

Résumé

Une injection d’homogénats et d’extraits partiellement purifiés du complexe cerveau – ganglion suboesophagien de femelles de même espèce ou de femelles de Heliothis zea ou encore une injection du neuropeptide synthétique activateur de la synthèse de phéromone (PBAN) provoque la production de la phéromone sexuelle chez les femelles de Heliothis virescens (F.) et de H. subflexa (Gn.) durant la photophase, une période où la phéromone n’est pas normalement produite. Chez H. virescens, la quantité de (Z)-11-hexadéccnal, la constituant principal de la phéromone chez les deux espèces, augmente durant les 60 premières minutes après l’injection de PBAN pour ensuite se stabiliser et décliner. Chez H. subftexa, la teneur de cet aldéhyde augmente pendant les 120 premières minutes et commence à décroître après 240 min, Les extracts de cerveau – ganglion suboesophagien qui ne contenaient pas d’aminés biogéniques produites par le système nerveux ont provoqué autant de production de phéromone que ceux qui en contenaient. Des dosages indiquent que la dose optimale de PBAN synthétique pour la production de phéromone lors d’une incubation de 60 min est de 5,0 pmol. Le maintien de la production de phéromone in vivo exige une stimulation continuelle de la glande à phéromone.

[Traduit par la rédaction]

Type
Articles
Information
The Canadian Entomologist , Volume 125 , Issue 2 , April 1993 , pp. 355 - 366
Copyright
Copyright © Entomological Society of Canada 1993

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