Hostname: page-component-586b7cd67f-r5fsc Total loading time: 0 Render date: 2024-11-24T10:33:13.405Z Has data issue: false hasContentIssue false
  • English
  • Français

IDENTIFICATION OF HOST ORIGIN OF PARASITES OF BARK BEETLES (COLEOPTERA: SCOLYTIDAE) BY FATTY ACID COMPOSITION1

Published online by Cambridge University Press:  31 May 2012

Louis H. Kudon  and
C. Wayne Berisford
Louis H. Kudon
Affiliation:
Department of Entomology, University of Georgia, Athens 30602
C. Wayne Berisford
Affiliation:
Department of Entomology, University of Georgia, Athens 30602
Get access Rights & Permissions [Opens in a new window]

Abstract

The free fatty acid composition of the southern pine beetle, Dendroctonus frontalis Zimmermann, and associated bark beetles was determined by gas-liquid chromatography. The lipid composition of several species of hymenopterous parasites matched the bark beetle hosts from which they were reared. Lipids from field collected parasites were compared with the lipid composition of possible bark beetle hosts to determine host of origin. Parasites ovipositing on a host were usually found to have a lipid composition matching that host. Approximately 20% of the parasites that were observed attempting to parasitize the southern pine beetle apparently developed on other bark beetle hosts.

Résumé

La composition en acides gras libres du dendroctone méridional du pin, Dendroctonus frontalis Zimmermann, et d’autres scolytes qui lui sont associés a été déterminée par chromatographie en phase gazeuse (GLC). La composition lipidique de plusieurs espèces d’hyménoptères parasites correspond à celle des scolytes leur ayant servi d’hôtes. Les lipides de parasites récoltés sur le terrain ont été comparés à ceux de scolytes leur ayant possiblement servi d’hôtes afin de déterminer l’hôte dont ils provenaient. Les parasites déposant leurs œufs sur un hôte montrent généralement une composition lipidique correspondant à celle de cet hôte. Environ 20% des parasites observés en train de parasiter D. frontalis provenaient apparemment d’autres espèces de scolytes.

Type
Articles
Information
The Canadian Entomologist , Volume 113 , Issue 3 , March 1981 , pp. 205 - 212
Copyright
Copyright © Entomological Society of Canada 1981

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Berisford, C. W. 1974. Hymenopterous parasitoids of the eastern juniper bark beetle, Phloeosinus dentatus (Coleoptera: Scolytidae). Can. Ent. 106: 869–872.Google Scholar
Berisford, C. W., Kulman, H. M., and Pienkowski, R. C.. 1970. Notes on the biologies of hymenopterous parasites of Ips spp. bark beetles in Virginia (Coleoptera: Scolytidae). Can. Ent. 102: 484–490.CrossRefGoogle Scholar
Bligh, E. G. and Dyer, W. J.. 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37(8): 911–916.CrossRefGoogle ScholarPubMed
Kudon, L. H. and Berisford, C. W.. 1980. Influence of brood hosts on host preferences of bark beetle parasites. Nature (Lond.) 283: 288–290.Google Scholar
Morrison, W. R. and Smith, L. M.. 1964. Preparation of fatty acid methyl esters and dimethylacetates from lipids with boron fluoride-methanol. J. Lipid Res. 5: 600–608.CrossRefGoogle ScholarPubMed
Sokal, R. R. 1961. Distance as a measure of taxonomic similarity. Syst. Zool. 10: 70–74.Google Scholar
Sokal, R. R. and Sneath, P. H.. 1963. Numerical Taxonomy. Freeman, San Francisco and London. p. 147.Google Scholar
Thompson, S. N. 1973. A review and comparative characterization of the fatty acid compositions of seven insect orders. Comp. Biochem. Physiol. 45B: 467–482.Google Scholar
Thompson, S. N. and Barlow, J. S.. 1972 a. Presence and synthesis of a 20 carbon monounsaturated fatty acid, 9 eicosenoic acid and other fatty acids, in Galleria mellonella (Lepidoptera: Pyralidae). Ann. ent. Soc. Am. 65(5): 1020–1023.CrossRefGoogle Scholar
Thompson, S. N. and Barlow, J. S.. 1972 b. Influence of host fatty acid composition on that of ichneumonid and chalcidoid wasps. J. Parasitol. 58(4): 836–839.Google Scholar
Thompson, S. N. and Barlow, J. S.. 1972 c. Synthesis of fatty acids by the parasite Exeristes comstockii (Hymenoptera) and two hosts, Galleria mellonella (Lepidoptera) and Lucilia sericata (Diptera). Can. J. Zool. 50: 1105–1110.Google Scholar
Thompson, S. N. and Barlow, J. S.. 1973. The inconsistent phospholipid fatty acid composition in an insect parasitoid, Itoplectis conquisitor (Say) (Lepidoptera: Pyralidae). Comp. Biochem. Physiol. 44B: 59–64.Google Scholar
Thompson, S. N. and Barlow, J. S.. 1974. The fatty acid composition of parasitic Hymenoptera and it's possible biological significance. Ann. ent. Soc. Am. 67: 627–632.CrossRefGoogle Scholar
Thompson, S. N. and Barlow, J. S.. 1976. Regulation of lipid metabolism in the insect parasite, Exeristes roborator (Fabricius). J. Parasitol. 62(2): 303–306.Google Scholar
Thompson, S. N. and Bennett, R. B.. 1971. Oxidation of fat during flight of male Douglas fir beetles, Dendroctonus pseudotsugae. J. Insect Physiol. 17: 1555–1563.CrossRefGoogle Scholar
Thompson, S. N. and Johnson, J.. 1978. Further studies on lipid metabolism in the insect parasite, Exeristes roborator (Fabricius). J. Parasitol. 64(4): 731–740.Google Scholar