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GENETICS OF GLOSSINA MORSITANS MORSITANS (DIPTERA: GLOSSINIDAE): II. ELECTROPHORETIC BANDING PATTERNS OF MIDGUT ALKALINE PHOSPHATASE

Published online by Cambridge University Press:  31 May 2012

R. H. Gooding  and
B. M. Rolseth
R. H. Gooding
Affiliation:
Department of Entomology, University of Alberta, Edmonton T6G 2E3
B. M. Rolseth
Affiliation:
Department of Entomology, University of Alberta, Edmonton T6G 2E3
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Abstract

The digestive section of the midgut of Glossina morsitans morsitans Westwood contains a phosphatase with a pH optimum of approximately 9.2 and with low substrate specificity; the enzyme was classified as an alkaline phosphatase (E.C. 3.1.3.1).

Polyacrylamide gel (6%) electrophoresis (at pH 8.9) of the digestive portion of the midguts of adult G. morsitans morsitans revealed three alkaline phophatase phenotypes. Midgut phosphatase was postulated to be under control of a single locus (designated alkph) with two alleles. Gene frequencies were in Hardy-Weinberg equilibrium in two laboratory populations while a third, highly inbred population had only one phenotype. Phenotype frequencies were not significantly different among females of various ages from the Edmonton colony. Breeding experiments provided direct evidence for single locus control of midgut phosphatase.

Type
Articles
Information
The Canadian Entomologist , Volume 110 , Issue 12 , December 1978 , pp. 1241 - 1246
Copyright
Copyright © Entomological Society of Canada 1978

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