Hostname: page-component-cd9895bd7-fscjk Total loading time: 0 Render date: 2024-12-26T04:57:20.654Z Has data issue: false hasContentIssue false
  • English
  • Français

GENETICS OF GLOSSINA MORSITANS MORSITANS (DIPTERA: GLOSSINIDAE): II. ELECTROPHORETIC BANDING PATTERNS OF MIDGUT ALKALINE PHOSPHATASE

Published online by Cambridge University Press:  31 May 2012

R. H. Gooding  and
B. M. Rolseth
R. H. Gooding
Affiliation:
Department of Entomology, University of Alberta, Edmonton T6G 2E3
B. M. Rolseth
Affiliation:
Department of Entomology, University of Alberta, Edmonton T6G 2E3
Get access Rights & Permissions [Opens in a new window]

Abstract

The digestive section of the midgut of Glossina morsitans morsitans Westwood contains a phosphatase with a pH optimum of approximately 9.2 and with low substrate specificity; the enzyme was classified as an alkaline phosphatase (E.C. 3.1.3.1).

Polyacrylamide gel (6%) electrophoresis (at pH 8.9) of the digestive portion of the midguts of adult G. morsitans morsitans revealed three alkaline phophatase phenotypes. Midgut phosphatase was postulated to be under control of a single locus (designated alkph) with two alleles. Gene frequencies were in Hardy-Weinberg equilibrium in two laboratory populations while a third, highly inbred population had only one phenotype. Phenotype frequencies were not significantly different among females of various ages from the Edmonton colony. Breeding experiments provided direct evidence for single locus control of midgut phosphatase.

Type
Articles
Information
The Canadian Entomologist , Volume 110 , Issue 12 , December 1978 , pp. 1241 - 1246
Copyright
Copyright © Entomological Society of Canada 1978

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Barka, T. 1961. Studies of acid phosphatase. I. Electrophoretic separation of acid phosphatase of rat liver on polyacrylamide gels. J. Histochem. Cytochem. 9: 542–547.CrossRefGoogle ScholarPubMed
Chen, P. S., Toribara, T. Y., and Warner, H.. 1956. Microdetermination of phosphorus. Anal. Chem. 28: 1756–1758.CrossRefGoogle Scholar
Dixon, W. J. and Massey, F. J.. 1957. Introduction to statistical analysis. McGraw-Hill, New York. xiii + 488 pp.Google Scholar
Geest, L. P. S. van der and Kawooya, J.. 1975. Genetic variation in some enzyme systems in the tsetse fly Glossina morsitans. Entornologia exp. appl. 18: 508–514.CrossRefGoogle Scholar
Gooding, R. H. and Rolseth, B. M.. 1976. Digestive processes of haematophagous insects. XI. Partial purification and some properties of six proteolytic enzymes from the tsetse fly Glossina morsitans morsitans Westwood (Diptera: Glossinidae). Can. J. Zool. 54: 1950–1959.CrossRefGoogle ScholarPubMed
Rolseth, B. M. and Gooding, R. H.. 1978. Genetics of Glossina morsitans morsitans (Diptera: Glossinidae). I. Electrophoretic banding patterns of xanthine oxidase and aldehyde oxidase. Can. Ent. 110: 1233–1239.CrossRefGoogle Scholar