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Genetic divergence among populations of Pissodes yunnanensis (Coleoptera: Curculionidae) in southwestern China

Published online by Cambridge University Press:  02 April 2012

Hongrui Zhang ,
David W. Langor ,
Hui Ye ,
Zhengyue Li  and
Richard D. Laffin
Hongrui Zhang
Affiliation:
Faculty of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China
David W. Langor*
Affiliation:
Canadian Forest Service, Northern Forestry Centre, 5320 122nd Street, Edmonton, Alberta, Canada T6H 3S5
Hui Ye
Affiliation:
Department of Biology, Yunnan University, Kunming 650091, Yunnan Province, China
Zhengyue Li
Affiliation:
Faculty of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China
Richard D. Laffin
Affiliation:
Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada T6E 2E9
*
1Corresponding author (e-mail: [email protected]).
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Abstract

The bark weevil, Pissodes yunnanensis Langor and Zhang, is an important pest of young Yunnan pine, Pinus yunnanensis Franchet (Pinaceae), in southwestern China. Populations of the host and weevil are disjunct, especially in the northwestern part of the range in Yunnan province. To estimate maternal gene flow and examine the genetic structure of Pissodes yunnanensis, we sequenced an 840-bp fragment of the mitochondrial cytochrome c oxidase I (COI) gene in 60 individuals from 7 populations in Yunnan and Guizhou provinces. Percent divergence among populations ranged from 0.001% to 2.1%. Nested clade analysis of 21 haplo types showed evidence of genetic structuring that is inferred to be primarily due to allopatric fragmentation and a low level of gene flow. Analysis of molecular variance also showed significant genetic structuring and restricted gene flow among weevil populations, especially between northwestern and eastern regions of the range. Such genetic structuring may be important for pest management programs. Phylogenetic analyses comparing the same 761-bp sequence among P. yunnanensis, Pissodes punctatus Langor and Zhang from southwestern China, and five Nearctic species of Pissodes showed that (i) the two Chinese species were most closely related to each other, (ii) the four species in the P. strobi (Peck) species group formed a distinct clade, and (iii) these two clades were more similar to each other than to the Nearctic species P. affinis Randall.

Résumé

Le charançon du tronc Pissodes yunnanensis Langor et Zhang est un important ravageur de jeunes pins du Yunnan, Pinus yunnanensis Franchet (Pinaceae), dans le sud-ouest de la Chine. Les populations de l'hôte et du charançon sont séparées, particulièrement dans la portion nord-ouest de leur répartition dans la province du Yunnan. Afin d'estimer le flux génique maternel et d'examiner la structure génétique chez Pissodes yunnanensis, nous avons séquencé un fragment de 840 pb du gène mitochondrial de la sous-unité I de la cytochrome oxydase c (COI) chez 60 individus appartenant à 7 populations dans les provinces de Yunnan et de Guizhou. Le pourcentage de divergence entre les populations varie de 0,001 % à 2,1 %. Une analyse cladistique emboîtée de 21 haploptypes donne des indications d'une structuration génétique que nous croyons être principalement due à la fragmentation allopatrique et à un faible taux de flux génique. L'analyse de la variance moléculaire montre aussi une importante structuration génétique et un flux génique réduit au sein des populations de charançons, particulièrement entre les régions du nord-ouest et de l'est de l'aire de répartition. Une telle structuration génétique peut avoir des conséquences sur les programmes de lutte contre ces ravageurs. Des analyses phylogénétiques qui comparent la même séquence de 761 pb chez P. yunnanensis, Pissodes punctatus Langor et Zhang du sud-ouest de la Chine et cinq espèces néarctiques de Pissodes révèlent que (i) les deux espèces chinoises sont celles qui sont les plus apparentées l'une à l'autre, (ii) les quatre espèces du groupe d'espèces de P. strobi (Peck) forment un clade distinct et (iii) ces deux clades sont plus semblables l'un à l'autre qu'ils ne le sont à l'espèce néarctique P. affinis Randall.

[Traduit par la Rédaction]

Type
Articles
Information
The Canadian Entomologist , Volume 139 , Issue 3 , June 2007 , pp. 308 - 318
Copyright
Copyright © Entomological Society of Canada 2007

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