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Genetic and morphometric variations in the Lebanese populations of the flower-head-infesting fruit fly, Terellia serratulae (Diptera: Tephritidae)

Published online by Cambridge University Press:  29 July 2016

S.G. Haddad ,
C.A. Smith ,
M.S. Al-Zein  and
K.M. Knio
S.G. Haddad
Affiliation:
Biology Department, Faculty of Arts & Sciences, American University of Beirut, PO Box 11-0236, Riad El Solh, Beirut, Lebanon
C.A. Smith
Affiliation:
Biology Department, Faculty of Arts & Sciences, American University of Beirut, PO Box 11-0236, Riad El Solh, Beirut, Lebanon
M.S. Al-Zein
Affiliation:
Biology Department, Faculty of Arts & Sciences, American University of Beirut, PO Box 11-0236, Riad El Solh, Beirut, Lebanon
K.M. Knio*
Affiliation:
Biology Department, Faculty of Arts & Sciences, American University of Beirut, PO Box 11-0236, Riad El Solh, Beirut, Lebanon
*
2Corresponding author (e-mail: [email protected]).
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Abstract

The polyphagous fruit fly, Terellia serratulae (Linnaeus) (Diptera: Tephritidae), exploits hosts belonging to three genera of thistles: Carduus Linnaeus, Cirsium Miller, and Picnomon Adanson (Asteraceae). The difference in phenology among its hosts suggests intraspecific variation. Comparative morphometric and genetic studies revealed differences among its populations. Adults reared from different hosts showed intraspecific morphological variations. Canonical discriminant analysis based on two head and four wing measurements divided the adults into four distinct clusters with 70% accuracy, reflecting four host-associated populations. The most useful predictors in distinguishing adults associated with the different host plants were wing width and head length, in addition to ovipositor length for females. Only the ovipositor tip in females reared from Picnomon acarna (Linnaeus) Cassini was clearly distinct. Mitochondrial DNA sequencing revealed genetic differentiation among the different populations of T. serratulae with the P. acarna-associated population being most distinct. Sequencing a region of the mtND1 gene and mtCOXI gene revealed nine and seven haplotypes, respectively. Surprisingly, haplotype sequences of flies emerging from P. acarna showed a sequence divergence of over 3% for both genes. This study provides morphometric and molecular evidence supporting that the Lebanese T. serratulae population associated with P. acarna most likely constitutes a distinct host race.

Type
Biodiversity & Evolution
Information
The Canadian Entomologist , Volume 149 , Issue 1 , February 2017 , pp. 73 - 88
Copyright
© Entomological Society of Canada 2016 

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Footnotes

Subject Editor: Chris Schmidt

1

Equal contribution.

References

Baloch, G.M. and Khan, A.G. 1973. Biological control of Carduus sp. II. Phenology, biology, and host specificity of Terellia serratulae L. (Diptera: Trypetid). Technical Bulletin of the Commonwealth Institute of Biological Control, 16: 11–22.Google Scholar
Berlocher, S.H. and Feder, J.L. 2002. Sympatric speciation in phytophagous insects: moving beyond controversy? Annual Review of Entomology, 47: 773–815.Google Scholar
Boulétreau-Merle, J., Allemand, R., Cohet, Y., and David, J.R. 1982. Reproductive strategy in Drosophila melanogaster: significance of a genetic divergence between temperate and tropical populations. Oecologia, 53: 323–329.Google Scholar
Cognato, A.I. 2006. Standard percent DNA sequence difference for insects does not predict species boundaries. Journal of Economic Entomology, 99: 1037–1045.Google Scholar
Drès, M. and Mallet, J. 2002. Host races in plant-feeding insects and their importance in sympatric speciation. Philosophical Transactions: Biological Sciences, 357: 471–492.CrossRefGoogle ScholarPubMed
Feder, J.L. and Filchak, K.E. 1999. It’s about time: the evidence for host plant-mediated selection in the apple maggot fly, Rhagoletis pomonella, and its implications for fitness trade-offs in phytophagous insects. Entomologia Experimentalis et Applicata, 91: 211–225.Google Scholar
Fletcher, B.S. 1989. Life history strategies of tephritid fruit flies. In Fruit flies: their biology, natural enemies and control. Edited by A.S. Robinson and G. Hooper. Elsevier Science Publishers, Amsterdam, The Netherlands. Pp. 195–208.Google Scholar
Freidberg, A. and Kugler, J. 1989. Fauna Palaestina, Insecta IV – Diptera: Tephritidae. Israel Academy of Sciences and Humanities, Jerusalem, Israel.Google Scholar
French, N., Yu, S., Biggs, P., Holland, B., Fearnhead, P., Binney, B., et al. 2013. Evolution of Campylobacter species in New Zealand. In Campylobacter ecology and evolution. Edited by S.K. Sheppard and G. Méric. Horizon Scientific Press, Norfolk, United Kingdom. Pp. 221–240.Google Scholar
Goeden, R.D. 1985. Host-plant relations of Trupanea species (Diptera: Tephritidae) in southern California. Proceedings of the Entomological Society of Washington, 87: 564–571.Google Scholar
Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41: 95–98.Google Scholar
Hebert, P.D.N., Cywinska, A., Ball, S.L., and deWaard, J.R. 2003. Biological identifications through DNA barcodes. Proceedings of the Royal Society of London Series B, 270: 313–321.Google Scholar
Kinkorova, J. 1991. Life histories of Terellia serratulae and Urophora stylata (Diptera: Tephritidae) and their co-occurrence on Cirsium vulgare . Acta Entomologica Bohemoslovica, 88: 293–298.Google Scholar
Knio, K.M., Kalash, S.H., and White, I.M. 2002. Flowerhead-infesting fruit flies (Diptera: Tephritidae) on thistles (Asteraceae) in Lebanon. Journal of Natural History, 36: 617–629.Google Scholar
Knio, K.M., White, I.M., and Al-Zein, M.S. 2007. Host-race formation in Chaetostomella cylindrica (Diptera: Tephritidae): morphological and morphometric evidence. Journal of Natural History, 41: 1697–1715.CrossRefGoogle Scholar
Korneyev, V. and Dirlbek, J. 2000. The fruit flies (Diptera: Tephritidae) of Syria, Jordan and Iraq. Studia Dipterologica, 7: 463–482.Google Scholar
Michaelis, H. 1986. Competition of Urophora stylata F. and Terellia serratulae L. (Diptera: Tephritidae) in the flowerheads of Cirsium vulgare . Pest Control: Operations and Systems Analysis in Fruit Fly Management, 11: 191–199.Google Scholar
Post, G.E. 1933. Flora of Syria, Palestine, and Sinai. American University of Beirut Press, Beirut, Lebanon.Google Scholar
R Development Core Team. 2008. R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. Available from http://www.R-project.org [accessed from 19 March 2016].Google Scholar
Redfern, M. 1968. The natural history of spear thistle-heads. Field Studies, 2: 669–717.Google Scholar
Sayar, N., Smith, C., White, I.M., and Knio, K. 2009. Terellia fuscicornis (Diptera: Tephritidae): biological and morphological adaptation on artichoke and milk thistle. Journal of Natural History, 43: 1159–1181.Google Scholar
Sharp, P.M. and Li, W.H. 1989. On the rate of DNA sequence evolution in Drosophila . Journal of Molecular Evolution, 28: 398–402.Google Scholar
Smith, C.A., Al-Zein, M.S., Sayar, N.P., and Knio, K.M. 2009. Host races in Chaetostomella cylindrica (Diptera: Tephritidae): genetic and behavioral evidence. Bulletin of Entomological Research, 99: 425–432.Google Scholar
Smith, P.T., McPheron, B.A., and Kambhampati, S. 2002. Phylogenetic analysis of mitochondrial DNA supports the monophyly of Dacini fruit flies (Diptera: Tephritidae). Annals of the Entomological Society of America, 95: 658–664.Google Scholar
SPSS Inc. 2007. SPSS for Windows, version 16.0. SPSS Inc., Chicago, Illinois, United States of America.Google Scholar
Stireman, J.O., Nason, J.D., and Heard, S.B. 2005. Host-associated genetic differentiation in phytophagous insects: general phenomenon or isolated exceptions? Evidence from a goldenrod-insect community. Evolution, 59: 2573–2587.Google Scholar
Stoffolano, J. 1989. Structure and function of the ovipositor of tephritids. In Fruit flies of economic importance. Edited by R. Cavalloro. A.A. Balkema, Rotterdam, The Netherlands. Pp. 141–146.Google Scholar
Tauber, C.A. and Tauber, M.J. 1989. Sympatric speciation in insects: perception and perspective. In Speciation and its consequences. Edited by D. Otte and J.E. Endler. Sinauer Associates, Sunderland, Massachusetts, United States of America. Pp. 307–344.Google Scholar
Whipple, A.V., Abrahamson, W.G., Khamiss, M.A., Heinrich, P.L., Urian, A.G., and Northridge, E.M. 2009. Host-race formation: promoted by phenology, constrained by heritability. Journal of Evolutionary Biology, 22: 793–804.Google Scholar
White, I.M. 1988. Tephritid flies (Diptera: Tephritidae). Handbook for the identification of British insects. Royal Entomological Society of London, 10: 1–134.Google Scholar
Zwölfer, H. 1972. Investigations on Chaetorellia species associated with C. solstitialis. In Weed project for the University of California, Division of Biological Control. Report 7. Commonwealth Institute of Biological Control, Delémont, Switzerland.Google Scholar
Zwölfer, H. 1988. Evolutionary and ecological relationships among the insect fauna of thistles. Annual Review of Entomology, 33: 103–122.Google Scholar
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