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EXTRACTION AND BIOASSAY OF FEMALE SEX PHEROMONE OF WHITE-MARKED TUSSOCK MOTH, ORGYIA LEUCOSTIGMA (LEPIDOPTERA: LYMANTRIIDAE)1

Published online by Cambridge University Press:  31 May 2012

G. G. Grant
G. G. Grant
Affiliation:
Insect Pathology Research Institute, Canadian Forestry Service, Sault Ste. Marie, Ontario
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Abstract

Factors affecting the extraction and bioassay of the sex pheromone of white-marked tussock moths were evaluated. Dipping the female pheromone glands in a solvent (preferably hexane) to produce a wash containing active material was a particularly effective and reproducible method of obtaining a potent extract. These were most active if obtained from virgin females < 2 days old. Extracts from recently mated females were only slightly less active than those from virgin females. Males were most responsive in bioassays when 2 to 3 days old. The maximum percentage response of males was obtained at 1 × 10−4 FE (female equivalents); beyond this concentration the percentage response decreased. Characteristically, extracts lost their potency within 1–3 weeks of preparation. It is suggested that either an inhibitor or loss of a chemical component of the sex pheromone causes an extract to become inactive.

Résumé

L’auteur évalua sur la Chenille à houppes blanches les facteurs affectant l’extraction et le bio-essai de la phéromone sexuelle d’individus striés de blanc. Le trempage de glandes femelles à phéromone dans un solvant (préférablement de l’hexane) afin de produire une mixture contenant du matériel actif s’avéra une méthode efficace et répétable d’obtenir l’extrait actif. Celui-ci fut le plus actif lorsque obtenu de femelles vierges de deux jours ou moins. Les extraits provenant de femelles récemment accouplées étaient légèrement moins actifs. Les mâles produisaient le plus lors de bio-essais lorsque âgés de 2 à 3 jours. Le pourcentage de rendement maximal des mâles fut obtenu à 1 × 10−4 EF (équivalents en femelles); passé cette concentration, le pourcentage de rendement diminua Une à trois semaines après leur préparation l’activité des extraits diminua de façon caractéristique. Ceci est probablement causé par un inhibiteur ou par le perte d’un composant chimique de la phéromone sexuelle.

Type
Articles
Information
The Canadian Entomologist , Volume 107 , Issue 3 , March 1975 , pp. 303 - 309
Copyright
Copyright © Entomological Society of Canada 1975

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References

Acree, F. Jr., Beroza, M., Holbrook, R. F., and Haller, H. L.. 1959. The stability of hydrogenated gypsy moth sex attractant. J. econ. Ent. 52: 82–85.Google Scholar
Bartell, R. J. and Roelofs, W. L.. 1973. Evidence for natural secondary compounds which modify the response of males of the red-banded leaf-roller to female sex pheromone. Ann. ent. Soc. Am. 66: 481–483.Google Scholar
Beroza, M. 1967. Nonpersistent inhibitor of the gypsy moth sex attractant in extracts of the insect. J. econ. Ent. 60: 875–876.CrossRefGoogle Scholar
Bierl, B. A., Beroza, M., and Collier, C. W.. 1970. Potent sex attractant of the gypsy moth: Its isolation, identification, and synthesis. Science 170: 87–89.CrossRefGoogle Scholar
Burton, R. L. 1970. A low-cost artificial diet for the corn earworm. J. econ. Ent. 63: 1969–1970.CrossRefGoogle Scholar
Cardé, R. T., Roelofs, W. L., and Doane, C. C.. 1973. Natural inhibitor of the gypsy moth sex attractant. Nature 241: 474–475.CrossRefGoogle Scholar
Doane, C. C. 1968. Aspects of mating behaviour of the gypsy moth. Ann. ent. Soc. Am. 61: 768–773.CrossRefGoogle Scholar
Grant, G. G., Brady, U. E., and Brand, J. M.. 1972. Male armyworm scent brush secretion: Identification and electroantennogram study of major components. Ann. ent. Soc. Am. 65: 1224–1227.CrossRefGoogle Scholar
Lawrence, L. A. and Bartell, R. J.. 1972. The effect of age on calling behaviour of virgin females of Epiphyas postvittana (Lepidoptera) and on their pheromone content and ovarian development. Entomologia exp. appl. 15: 455–464.CrossRefGoogle Scholar
Percy, J. E., Gardiner, E. J., and Weatherston, J.. 1971. Studies of physiologically active arthropod secretions. VI. Evidence for a sex pheromone in female Orgyia leucostigma (Lepidoptera: Lymantriidae). Can. Ent. 103: 706–712.CrossRefGoogle Scholar
Shorey, H. H., McFarland, S. V., and Gaston, L. K.. 1968. Sex pheromones of noctuid moths. XIII. Changes in pheromone quantity, as related to reproductive age and mating history, in females of seven species of Noctuidae (Lepidoptera). Ann. ent. Soc. Am. 61: 372–376.CrossRefGoogle Scholar
Sower, L. L., Coffelt, J. A., and Vick, K. W.. 1973. Sex pheromone: A simple method of obtaining relatively pure material from females of five species of moths. J. econ. Ent. 66: 1220–1222.CrossRefGoogle Scholar