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Effects of sublethal doses of chlorfluazuron on the biochemical constituents of eggs of Spodoptera litura (Lepidoptera: Noctuidae)

Published online by Cambridge University Press:  03 January 2012

F. Perveen*
Affiliation:
Laboratory of Applied Entomology, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan
*
2Corresponding author (e-mail: [email protected]).

Abstract

The effects of sublethal doses (LD10: 1.00 ng/larva; LD30: 3.75 ng/larva) of chlorfluazuron on the biochemical constituents of eggs of the tobacco cutworm, Spodoptera litura (F.), are described. Chlorfluazuron was applied topically to fifth-instar larvae and the subsequent adults were allowed to mate according to larval treatment (LD10-treated female × LD10-treated male and LD30-treated female × LD30-treated male). Biochemical constituents of eggs resulting from these pairings were analyzed at various stages of embryonic development. Compared with controls, LD10 or LD30 reduced egg constituents as follows: protein (min.–max.) by 32.8%–34.5% or 62.0%–67.3%, lipid by 33%–34% or 62%–67%, carbohydrates by 30%–39% or 60%–67%, DNA by 33%–40% or 60%–69%, RNA by 31%–34% or 59%–67%, and ecdysteroid by 22%–83% or 28%–92%, respectively. The relative proportions of constituents in control eggs were as follows: protein > lipid > carbohydrate, and RNA > DNA. Three low and three high peaks in ecdysteroid titres were observed. Compared with controls, all peaks were reduced in LD10 or LD30 eggs as follows: low peaks: 1st (at 8 h): 32% or 66%; 2nd (at 16 h): 33% or 67%; 3rd (at 52 h): 35% or 65%; high peaks: 1st (at 32 h): 83% or 92%; 2nd (at 64 h): 65% or 82%; 3rd (at 84 h): 84 h, 36% or 63%, respectively. In addition, the first two high peaks were delayed by 4 h in LD10 eggs and by 8 h in LD30 eggs compared with controls. Sublethal doses of chlorfluazuron reduced the amounts of biochemical constituents of eggs during embryogenesis in S. litura.

Résumé

Notre étude décrit les effets de doses sublétales (LD10 : 1,00 ng/larve; LD30 : 3,75 ng/larve) de chlorfluazuron sur les constituants biochimiques des œufs du ver gris commun, Spodoptera litura (F.). Nous avons procédé à un traitement topique des larves de cinquième stade au chlorfluazuron et avons laissé les adultes produits s’accoupler en fonction du traitement larvaire (femelle traitée par LD10 x mâle traité par LD10 et femelle traitée par LD30 x mâle traité par LD30). Nous avons analysé les constituants biochimiques des œufs produits par ces accouplements à diverses étapes du développement embryonnaire. Les constituants des œufs sont réduits par LD10 et LD30, les protéines (min.–max.) respectivement de 32,8 % – 34,5 % et de 62,0 % – 67,3 %, les lipides de 33 % – 34 % et de 62 % – 67 %, les hydrates de carbone de 30 % – 39 % et de 60 % – 67 %, l’ADN de 33 % – 40 % et de 60 % – 69 %, l’ARN de 31 % – 34 % et de 59 % – 67% et les ecdystéroïdes de 22 % – 83 % et de 28 % – 92 %. Les proportions relatives des constituants dans les œufs témoins sont par ordre protéines>lipides>hydrates de carbone et ARN>ADN. On observe trois maximums élevés et trois minimums plus bas dans les titres d’ecdystéroïdes. Par comparaison aux témoins, tous les maximums sont réduits chez les œufs traités par LD10 et LD30: les maximums bas, le premier à 8h respectivement de 32 % et de 66%, le second à 16 h de 33 et de 67 % et le troisième à 52 h de 35 % et de 65 %; les maximums élevés, le premier à 32 h respectivement de 83 % et de 92 %, le second à 64 h de 65 % et de 82 % et le troisième à 84 h de 36 % et de 63 %. De plus, il y a un délai de 4 h dans les deux premiers maximums élevés chez les œufs traités par LD10 et de 8h chez ceux traités par LD30 par comparaison aux témoins. Les doses sublétales de chlorfluazuron réduisent donc les quantités des constituants biochimiques des œufs durant l’embryogenèse chez S. litura.

[Traduit par la Rédaction]

Type
Articles
Copyright
Copyright © Entomological Society of Canada 2011

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