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Effects of diapause duration on postdiapause performance of spruce budworm (Lepidoptera: Tortricidae) during mass rearing on artificial diet

Published online by Cambridge University Press:  02 April 2012

Kees van Frankenhuyzen*
Affiliation:
Great Lakes Forestry Centre, Canadian Forest Service, Natural Resources Canada, 1219 Queen Street East, Sault Ste. Marie, Ontario, Canada P6A 2E5
Peter Ebling
Affiliation:
Great Lakes Forestry Centre, Canadian Forest Service, Natural Resources Canada, 1219 Queen Street East, Sault Ste. Marie, Ontario, Canada P6A 2E5
John Dedes
Affiliation:
Great Lakes Forestry Centre, Canadian Forest Service, Natural Resources Canada, 1219 Queen Street East, Sault Ste. Marie, Ontario, Canada P6A 2E5
Doug Pitt
Affiliation:
Great Lakes Forestry Centre, Canadian Forest Service, Natural Resources Canada, 1219 Queen Street East, Sault Ste. Marie, Ontario, Canada P6A 2E5
*
1 Corresponding author (e-mail: [email protected]).
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Abstract

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The spruce budworm, Choristoneura fumiferana (Clem.), has an obligatory winter dormancy period that lasts up to 10 months in the field. In the Great Lakes Forestry Centre rearing facility, neonate larvae spin hibernacula in cheesecloth, which is then stored at 2 °C for between 20 and 30 weeks. Although dormancy survival and synchrony of postemergence development are highest when larvae are stored in the cold for 16–35 weeks, it is not known how cold-storage duration affects spruce budworm performance once diapause has been completed. We exposed approximately 9250 second-instar larvae (belonging to three rearing cohorts) to 2 °C for 16, 19, 22, 25, 28, 31, 34, or 37 weeks and monitored various postdiapause performance variables. Increasing cold storage from 16 to 25 weeks or more resulted in small (approximately 10%) increases in dormancy survival and larval development rates (from second instar to pupation), a larger (up to 23%) increase in pupal mass and realized fecundity (number of eggs laid per female), and an increase of at least 25% in late-instar survival (from fifth instar to pupation). The only variable that was negatively affected was the pupal survival, but the decrease was relatively small. Therefore, storing diapausing larvae for at least 25 weeks optimizes postdiapause performance variables that are important for mass-rearing efficiency.

Résumé

La tordeuse des bourgeons de l’épinette (Choristoneura fumiferana (Clem.)) traverse durant l’hiver une période de diapause obligatoire qui peut durer jusqu’à 10 mois sur le terrain. Dans les installations d’élevage du Centre de foresterie des Grands Lacs, les chenilles néonates se tissent des hibernaculums dans la gaze, puis sont entreposées à 2 °C pendant 20–30 semaines. Même si le taux de survie à la diapause et le synchronisme du développement postémergence sont meilleurs chez les chenilles entreposées au froid pendant 16–35 semaines, nous ne connaissons pas les effets de la durée d’entreposage en chambre froide sur la performance de la tordeuse des bourgeons de l’épinette, une fois la diapause levée. Nous avons exposé environ 9250 chenilles du deuxième stade (issues de trois cohortes d’élevage différentes) à une température de 2 °C pendant 16, 19, 22, 25, 28, 31, 34 ou 37 semaines, puis avons surveillé diverses variables de la performance postdiapause. Nous avons fait passer la durée de conservation en chambre froide de 16–25 semaines ou plus, ce qui a entraîné de faibles (à peu près 10 %) augmentations des taux de survie à la diapause et de développement larvaire (depuis le deuxième stade jusqu’à la chrysalidation) ainsi qu’une augmentation plus importante (à peu près 23 %) de la masse des chrysalides et de la fécondité réalisée. Le dernier stade (du cinquième stade à la chrysalidation) présentait la plus forte augmentation (à peu près 25 % – 30 %). L’émergence des chrysalides est la seule variable qui ait subi des effets négatifs, mais la diminution était relativement faible. Par conséquent, la conservation en chambre froide de chenilles en diapause pendant au moins 25 semaines optimize les variables de la performance postdiapause qui contribuent de façon importante à l’efficacité de l’élevage de masse.

[Traduit par la Réduction]

Type
Articles
Copyright
Copyright © Entomological Society of Canada 2007

References

Chang, Y.-F., Tauber, M.J., and Tauber, C.A. 1996. Reproduction and quality of F1 offspring in Chrysoperla carnea: differential influence of quiescence, artificially induced diapause, and natural diapause. Journal of Insect Physiology, 42: 521528.CrossRefGoogle Scholar
Grisdale, G.D. 1970. An improved laboratory method for rearing large numbers of spruce budworm, Choristoneura fumiferana (Lepidoptera: Tortricidae). The Canadian Entomologist, 102: 11111117.CrossRefGoogle Scholar
Grisdale, D.G. 1984. A laboratory method for mass rearing the eastern spruce budworm, Choristoneura fumiferana. In Advances and challenges in insect rearing. Edited by King, E.G. and Leppla, N.C.. United States Department of Agriculture Research Service Technical Bulletin, pp. 223231.Google Scholar
Han, E.-N., and Bauce, E. 1993. Physiological changes and cold hardiness of spruce budworm, Choristoneura fumiferana (Clem.) during pre-diapause and diapause development under laboratory conditions. The Canadian Entomologist, 125: 10431053.CrossRefGoogle Scholar
Han, E.-N., and Bauce, E. 1995. Glycerol synthetis by diapausing larvae in response to the timing of low temperature exposure, and implications for overwintering survival of the spruce budworm. Journal of Insect Physiology, 41: 981985.CrossRefGoogle Scholar
Han, E.-N., and Bauce, E. 1996. Diapause development of spruce budworm larvae, Choristoneura fumiferana (Clem.) (Lepidoptera: Tortricidae), at temperatures favouring post-diapause development. The Canadian Entomologist, 128: 167169.CrossRefGoogle Scholar
Han, E.-N., and Bauce, E. 1997. Effects of early temperature exposure on diapause development of spruce budworm (Lepidoptera: Tortricidae). Environmental Entomology, 26: 307310.CrossRefGoogle Scholar
Hodek, I., and Hodkova, M. 1988. Multiple role of temperature during insect diapause: a review. Entomologia Experimentalis et Applicata, 29: 153165.CrossRefGoogle Scholar
Kroon, A., and Veenendaal, R.L. 1998. Trade-off between diapause and other life-history traits in the spider mite Tetranychus urticae. Ecological Entomology, 23: 298304.CrossRefGoogle Scholar
Littell, R.C., Milliken, G.A., Stroup, W.W., and Wolfinger, R.D. 1996. SAS® system for mixed models [computer program]. SAS Institute Inc., Cary, North Carolina.Google Scholar
McMorran, A. 1973. Effects of pre-storage treatment on survival of diapausing larvae of the spruce budworm, Choristoneura fumiferana (Lepidoptera: Tortricidae). The Canadian Entomologist, 105: 10051009.CrossRefGoogle Scholar
Minitab Inc. 2004. Statistical Software Release 14 [computer program]. Minitab Inc., State College, Pennsylvania.Google Scholar
Stehr, G. 1954. A laboratory method for rearing of the spruce budworm, Choristoneura fumiferana (Clem.) (Lepidoptera: Tortricidae). The Canadian Entomologist, 86: 423428.CrossRefGoogle Scholar
van Frankenhuyzen, K., Ebling, P., McCron, B., Ladd, T., Gauthier, D., and Vossbrinck, C. 2004. Occurrence of Cystosporogenes sp. (Protozoa: Microsporidia) in a multi-species insect production facility and its elimination from a colony of the eastern spruce budworm, Choristoneura fumiferanae (Clem.) (Lepidoptera: Tortricidae). Journal of Invertebrate Pathology, 87: 1628.CrossRefGoogle Scholar
Wang, X.-P., Xue, F.-S., Hua, A., and Ge, F. 2006. Effects of diapause duration on future reproduction in the cabbage beetle, Colephellus bowringi: positive or negative? Physiological Entomology, 31: 190196.Google ScholarPubMed
Wellington, W.G. 1950. Variations in silk-spinning and locomotor activities of larvae of the spruce budworm, Choristoneura fumiferana (Clem.) at different rates of evaporation. Transactions of the Royal Society of Canada, 44: 89101.Google ScholarPubMed