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A COMPARISON OF LABORATORY AND FIELD TESTS OF BACILLUS SPHAERICUS STRAIN 1593 AND BACILLUS THURINGIENSIS VAR. ISRAELENSIS AGAINST AEDES STIMULASS LARVAE (DIPTERA: CULICIDAE)1

Published online by Cambridge University Press:  31 May 2012

Stephen P. Wraight ,
Daniel Molloy  and
Patricia McCoy
Stephen P. Wraight
Affiliation:
Biological Survey, New York State Museum, The State Education Department, Cultural Education Center, Albany, New York 12230
Daniel Molloy
Affiliation:
Biological Survey, New York State Museum, The State Education Department, Cultural Education Center, Albany, New York 12230
Patricia McCoy
Affiliation:
Biological Survey, New York State Museum, The State Education Department, Cultural Education Center, Albany, New York 12230
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Abstract

Bacillus thuringiensis var. israelensis (serotype H-14) and B. sphaericus strain 1593 were tested against Aedes stimulons larvae in the laboratory and in 38-cm-diam, open-ended cylinders embedded in the bottom detritus of a woodland pool. Estimates of LC50 were lower against fourth instars in the field at a mean temperature of 15.9 °C than in the laboratory at 21.1 °C. The greater efficacy in the field was attributed to high daytime water temperatures (mean 20.5 °C) following treatment and exposure of the larvae to substantially greater amounts of toxic material in a larger volume of water than in the laboratory. The regression of probit on log10 concentration was not linear over the entire range of mortality caused by B. sphaericus, increasing the difficulty of estimation of LC values. Bacillus sphaericus was significantly less active than B. thuringiensis.

Résumé

Bacillus thuringiensis var. israelensis (sérotype H-14) et B. sphaericus souche 1593 ont été testés contre des larves d'Aedes stimulans au laboratoire et dans des cylindres ouverts de 38 cm de diamètre enfoncés dans les matériaux du fond d'un étang de boisé. Les estimés de la CL50 obtenus contre les larves de stade 4 sur le terrain à une température moyenne de 15.9 °C étaient inférieurs à ceux obtenus en laboratoire à 21.1 °C. L'efficacité plus grande sur le terrain fût attribuée aux températures diurnes de l'eau élevées (moyenne de 20.5 °C) observées après le traitement, et à l'exposition des larves à des quantités substantiellement plus grandes de matériel toxique dans un volume d'eau plus grand qu'au laboratoire. La régression des probits sur le log10 de la concentration n'était pas linéaire sur tout l'écart couvert par la mortalité due à B. sphaericus, rendant ainsi plus difficile l'estimation des valeurs de la CL. B. sphaericus s'est avéré significativement moins actif que B. thuringiensis.

Type
Articles
Information
The Canadian Entomologist , Volume 114 , Issue 1 , January 1982 , pp. 55 - 61
Copyright
Copyright © Entomological Society of Canada 1982

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