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CHARACTERIZATION OF HYDROPSYCHE SLOSSONAE (TRICHOPTERA: HYDROPSYCHIDAE) CAPTURE NET POLYPEPTIDES

Published online by Cambridge University Press:  31 May 2012

L. Tessier ,
J.L. Boisvert ,
L.B-M. Vought  and
J.O. Lacoursière
L. Tessier
Affiliation:
Département de chimie-biologie, Université du Québec à Trois-Rivières, C.P. 500, Trois-Rivières, Québec, Canada G9A 5H7
J.L. Boisvert*
Affiliation:
Département de chimie-biologie, Université du Québec à Trois-Rivières, C.P. 500, Trois-Rivières, Québec, Canada G9A 5H7
L.B-M. Vought
Affiliation:
Department of Ecology, University of Lund, Ecology Building, Lund, Sweden
J.O. Lacoursière
Affiliation:
Department of Ecology, University of Lund, Ecology Building, Lund, Sweden
*
1 Author to whom all corresponding should be addressed (E-mail: [email protected]).
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Abstract

The aim of this study was to characterize polypeptide components of the capture net spun by trichopteran larvae Hydropsyche slossonae (Banks) (Trichoptera: Hydropsychidae). Thirty-one polypeptide bands were identified by SDS – polyacrylamide gel electrophoresis (SDS–PAGE) from extracted net material, with molecular weights ranging from 8500 to 179 000. Comparison with published data on Bombyx mori (L.) (Lepidoptera: Bombycidae) silk, treated under similar denaturing conditions, shows that six low molecular weight polypeptides ranging between 8500 and 18 800 in the silk of H. slossonae are absent from that of B. mori; furthermore, two high molecular weight polypeptides (210 000 and 220 000) detected in the silk of B. mori are not present in that of H. slossonae. Differences between both groups are probably related to their mode of living and to the specific use of silk (in air versus under water). Our findings are consistent with the current trend in the literature that silk spun by aquatic and terrestrial insects, as well as those spun by different species, is apparently made of different biopolymers according to the protein constituents. Hence, the polypeptide characterization of silk, combined with sequence data and (or) antibodies cross-reactivity data, could represent a potential tool for taxonomic classification improvement of aquatic insects. These results could eventually be used to characterize hydropsychid capture net anomalies induced by environmental pollution.

Résumé

Le but de cette étude était de caractériser la nature des polypeptides présents dans la soie des filets de capture tissés par les larves d’Hydropsyche slossonae (Banks) (Trichoptera : Hydropsychidae). Trente et un polypeptides ont été identifiés suite à une extraction du matériel contenu dans les filets de capture par la technique d’électrophorèse sur gel d’acrylamide (SDS–PAGE), les poids moléculaires de ceux-ci variant entre 8500 et 179 000. Une comparaison avec des données publiées sur la soie de Bombyx mori (L.) (Lepidoptera : Bombycidae), dans des conditions d’extraction similaires, a révélé que six polypeptides de faible poids moléculaire, variant entre 8500 et 18 800, présents dans la soie d’H. slossonae étaient absents au niveau de la soie de B. mori. De plus deux polypeptides de haut poids moléculaire (210 000 et 220 000) mis en évidence dans la soie de B. mori n’étaient pas présents au niveau de la soie d’H. slossonae. Ces divergences entre les deux groupes taxonomiques sont possiblement reliées aux différences notables entre leur mode de vie et leur utilisation spécifique de la soie (dans l’air et dans l’eau). Nos résultats confirment la tendance actuelle dans la littérature où il appert que la soie produite par les insectes aquatiques et terrestres, ainsi que celle tissée par différentes espèces, n’est apparemment pas constituée de biopolymères similaires, et ce à l’égard des analyses poly-peptidiques. Ainsi, l’établissement des patrons polypeptidiques au niveau de la soie, combiné à des analyses de séquence et (ou) des analyses de réaction croisée à l’aide d’anticorps, pourrait s’avérer un outil d’une grande utilité afin d’améliorer la classification taxonomique des insectes aquatiques. Ces résultats seront éventuellement utilisés afin de caractériser les anomalies observables sur les filets de capture des larves d’Hydropsyche spp. suite à une pollution environnementale chronique.

Type
Articles
Information
The Canadian Entomologist , Volume 132 , Issue 1 , February 2000 , pp. 59 - 68
Copyright
Copyright © Entomological Society of Canada 2000

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