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CHANGES IN HEMOLYMPH PROTEINS IN RELATION TO DORMANCY OF BARBARA COLFAXIANA KFT. AND THE INFLUENCE OF ECDYSONE AND PRECOCENE ON THE PROTEIN COMPOSITION

Published online by Cambridge University Press:  31 May 2012

T.S. Sahota ,
F.G. Peet ,
A.J. Thomson  and
A. Ibaraki
T.S. Sahota
Affiliation:
Pacific Forestry Centre, 506 West Burnside Road, Victoria, British Columbia, Canada V8Z 1M5
F.G. Peet
Affiliation:
Pacific Forestry Centre, 506 West Burnside Road, Victoria, British Columbia, Canada V8Z 1M5
A.J. Thomson
Affiliation:
Pacific Forestry Centre, 506 West Burnside Road, Victoria, British Columbia, Canada V8Z 1M5
A. Ibaraki
Affiliation:
Pacific Forestry Centre, 506 West Burnside Road, Victoria, British Columbia, Canada V8Z 1M5
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Abstract

Hemolymph proteins of the Douglas-fir cone moth, Barbara colfaxiana (Kft.), in the year of pupation (year P) and the next year (year P + 1) were studied by visual examination and analysis of digitized images of isoelectric focussing patterns. Differences in these proteins between light and dark individuals in year P and P + 1 were not related to dormancy duration but reflected the extent of pharate-adult development. Hormone applications were more effective in changing hemolymph proteins of light insects than those of dark ones. Changes in the hemolymph proteins of light insects in year P + 1 were not very obvious by visual examination of gels but were readily detectable by digital image analysis and showed that development occurred in these insects through June-September leading to visible pigmentation differences observable in relation to dormancy commitments of these insects.

Résumé

Les caractéristiques des protéines de l’hémolymphe du perce-cône du Douglas [Barbara colfaxiana (Kft.)] en l’année de chrysalidation (année P) et en l’année suivante (année P + 1) ont été étudiées par examen visuel et analyse d’images numérisées de la migration des protéines obtenue par électrofocalisation. Les différences observées entre les individus clairs et foncés pour les années P et P + 1 n’étaient pas reliées à la durée de la dormance, mais reflétaient l’importance du développement de l’adulte au stade pharate. Des applications d’hormones ont mieux réussi à modifier les protéines de l’hémolymphe chez les insectes clairs que chez les foncés. Chez les insectes clairs en l’année P + 1 les modifications n’étaient pas très évidentes à l’observation visuelle des gels, mais étaient facilement décelables par analyse des images numériques. Elles ont indiqué que leur développement se poursuivait de juin à septembre et entraînait des différences visibles de pigmentation reliées à la dormance.

Type
Articles
Information
The Canadian Entomologist , Volume 118 , Issue 9 , September 1986 , pp. 839 - 847
Copyright
Copyright © Entomological Society of Canada 1986

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