Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-05T14:47:32.695Z Has data issue: false hasContentIssue false

CANDLING AND SIGHTING TECHNIQUES USED IN LASER INVESTIGATIONS OF THE EUROPEAN PINE SAWFLY, NEODIPRION SERTIFER (HYMENOPTERA: DIPRIONIDAE)

Published online by Cambridge University Press:  31 May 2012

Ronald W. Kobylnyk
Affiliation:
Department of Zoology, University of Guelph, Guelph, Ontario

Extract

Laser treatment of larval and pupal stages within cocoons of Neodiprion sertifer (Geoff.) requires an accurate assessment of viability and the antero-posterior alignment of the cocoon content. The use of the candling technique to assess qualitative features of avian eggs depends on the translucency of the egg shell and the differences in light transmission by other elements within it (Romanoff and Romanoff 1949). Transmitted-light methods have been employed for insects within cocoons by Cushman (1913) and Hanna (1935). A type of candling technique different from previous methods and which was developed in the Zoology Department, University of Guelph, enables N. sertifer cocoon content to be ascertained and is herein described.

Type
Articles
Copyright
Copyright © Entomological Society of Canada 1967

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bessis, M., and Burté, B.. 1965. Positive and negative chemotaxis as observed after the destruction of a cell by U.V. or laser microbeams. Texas Rep. Biol. Med. 23 (suppl. 1): 204212.Google ScholarPubMed
Cushman, R. A. 1913. The Calliephialtes parasite of the codling moth. J. agric. Res. 1: 211212.Google Scholar
Earle, K. M. et al. , 1965. Central nervous system effects of laser radiation. Fedn Proc. Fedn Am. Socs exp. Biol. 24(1, Part III, Suppl. 14): S–129139.Google ScholarPubMed
Fine, S. et al. , 1965. Interaction of laser radiation with biologic systems. I. Studies on interaction with tissues. Fedn Proc. Fedn Am. Socs exp. Biol. 24(1, Part III, Suppl. 14): S–3545.Google ScholarPubMed
Geeraets, W. J. et al. , 1965. Laser versus light coagulator: A fundoscopic study of chorioretinal injury as a function of exposure time. Fedn Proc. Fedn Am. Socs exp. Biol. 24(1, Part III, suppl. 14): S–4861.Google Scholar
Goldman, L., and Owens, P. L.. 1964. Chromosome studies in dermatology. Preliminary observations in some congenital and acquired dermatoses and in the effects with X-ray, Grenz and laser. Acta derm.-vener., Stockh. 44: 6875.Google Scholar
Hanna, A. D. 1935. Fertility and toleration at low temperatures in Euchalcidia caryobori Hanna. Bull. ent. Res. 26: 315322.CrossRefGoogle Scholar
Igelman, J. M., and Rotte, T.. 1965. Effects of laser radiation of tyrosinase. Fedn Proc. Fedn Am. Socs exp. Biol. 24 (1, Part III, suppl. 14): S–9496.Google ScholarPubMed
Lang, K. R. et al. , 1964. Lasers as tools for embryology and cytology. Nature 201 (4920): 675677.CrossRefGoogle ScholarPubMed
Malt, R. A. 1965. Effects of laser radiation on subcellular components. Fedn Proc. Fedn Am. Socs exp. Biol. 24(1, Part III, suppl. 14): S–122125.Google ScholarPubMed
Mendelson, J. A., and Ackerman, N. B.. 1965. Studies of biologically significant forces following laser irradiation. Fedn Proc. Fedn Am. Socs exp. Biol. 24 (1, Part III, suppl. 14): S–111115.Google ScholarPubMed
Romanoff, A. L., and Romanoff, A. J.. 1949. The avian egg. pp. 634640. J. Wiley and Sons Inc., New York.Google Scholar
Wilde, W. H. A. 1965. Laser effects on two insects. Can. Ent. 97: 8892.CrossRefGoogle Scholar
Zweng, H. C., and Flocks, M.. 1965. Clinical experiences with laser photocoagulation. Fedn Proc. Fedn Am. Socs exp. Biol. 24 (1, Part III, suppl. 14): S–6570.Google ScholarPubMed