The Byron Bog lies within the city limits of the City of London in southwestern Ontario. It is a relic bog lying about 300 miles south of the forested region of Ontario in which bogs are prevalent. It lies in the northern border of the Carolinian Zone.

The wooded slopes around the bog include trees characteristic of southern Ontario, including White Elm, Bur Oak, Hackberry, Silver Maple, Black Cherry and Choke Cherry. The slopes encircle a zone of damp woods with Alder Buckthorn, White Pine, White Birch, Larch, Black Spruce with an undergrowth of Purple Chokeberry, Huckleberry, Blueberry and Leather-leaf.