Hostname: page-component-cd9895bd7-q99xh Total loading time: 0 Render date: 2024-12-29T04:56:57.408Z Has data issue: false hasContentIssue false
  • English
  • Français

Assessment of Metarhizium anisopliae (Clavicipitaceae) and its vector, Scleroderma guani (Hymenoptera: Bethylidae), for the control of Monochamus alternatus (Coleoptera: Cerambycidae)

Published online by Cambridge University Press:  22 December 2014

M. Xu ,
F.Y. Xu ,
Y.P. Liu ,
Y.S. Pan  and
X.Q. Wu
M. Xu
Affiliation:
College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China Forestry Academy of Jiangsu Province, Nanjing 211153, Dongshanqiao, China
F.Y. Xu*
Affiliation:
Forestry Academy of Jiangsu Province, Nanjing 211153, Dongshanqiao, China
Y.P. Liu
Affiliation:
Forestry Academy of Jiangsu Province, Nanjing 211153, Dongshanqiao, China
Y.S. Pan
Affiliation:
Suqian Forestry Bureau, Suqian City 223800, China
X.Q. Wu
Affiliation:
College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China
*
1 Corresponding author (e-mail: [email protected]).
Get access Rights & Permissions [Opens in a new window]

Abstract

The pinewood nematode, Bursaphelenchus xylophilus (Steiner and Buhrer) Nickle (Aphelenchida: Parasitaphelenchidae), is a major disease of pines forests in China. Its vector, Monochamus alternatus Hope (Coleoptera: Cerambycidae), has been the focus of an intensive study to improve the efficiency of the parasitoid Scleroderma guani Xiao and Wu (Hymenoptera: Bethylidae) by using the parasitoid both as a larval parasitoid in its own right and additionally as a vector for the entomophagous pathogen Metarhizium anisopliae (Metchnikoff) Sorokin (Clavicipitaceae). Twenty-one M. anisopliae strains were screened and the best four tested for sporulation capabilities at a range of temperatures. The best strain was then evaluated at several sporulation concentrations to define the lethal concentration 50 (LC50) against the larvae of M. alternatus. Scleroderma guani was inoculated with the best strain and tested against larvae of M. alternatus, which were constrained singly in glass vials. Three S. guani females per vial infected with M. anisopliae (strain 789) were shown to cause the death of 100% of the M. alternatus larvae they were exposed to as compared exposures to just one or two infected S. guani females per vial. This demonstrates a successful interaction of two biocontrol agents for the control of the pinewood nematode vector M. alternatus.

Type
Insect Management
Information
The Canadian Entomologist , Volume 147 , Issue 5 , October 2015 , pp. 628 - 634
Copyright
© Entomological Society of Canada 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

Subject editor: Jianghua Sun

References

Burges, H.D. 1981. Microbial control of pests and plant diseases 1970–1980. Academic Press, New York, New York, United States of America.Google Scholar
Butt, T.M. 2002. Use of entomogenous fungi for the control of insect pests. In The Mycota. Agricultural applications. Edited by F. Kempken. Springer-Verlag, Heidelberg, Germany. Pp. 111–134.CrossRefGoogle Scholar
Chambers, D. 1977. Quality control in mass rearing. Annual Review of Entomology, 22: 289–308.CrossRefGoogle Scholar
Cloyd, R.A. 1999. The entomopathogenic fungus Metarhizium anisopliae [online]. Midwest Biological Control News 6(7). Available from: http://www.entomology.wisc.edu/mbcn/kyf607.html [accessed 28 October 2014].Google Scholar
Dwinell, L.D. 1997. The pinewood nematode: regulation and mitigation. Annual Review of Phytopathology, 35: 153–166.CrossRefGoogle ScholarPubMed
Ferron, P., Fargues, J., and Riba, G. 1991. Fungi as microbial insecticides against pest. In Handbook of applied mycology. Volume 2, Edited by D.K. Arora, L. Ajello, and K.G. Mukerji. Marcel Dekker, New York, New York, United States of America. Pp. 665–706.Google Scholar
Gandolfi, M., Mattiacci, L., and Dorn, S. 2003. Mechanisms of behavioral alterations of parasitoids reared in artificial systems. Journal of Chemical Ecology, 29: 1871–1887.CrossRefGoogle ScholarPubMed
He, X., Chen, S., and Huang, J. 2005. Preliminary screening of virulent strains of Metarhizium anisopliae against Monochamus alternatus . Acta Entomologica Sinica, 48: 975–981.Google Scholar
Kondo, E.F., Linit, M.J., Smith, M.T., Bolla, R., Winter, R.E., and Dropkin, V.H. 1982. Pine wilt disease: nematological, entomological and biochemical investigations. Bulletin SB-372, University of Missouri Agriculture Experiment, Station, Columbia, Missouri, United States of America P. 58.Google Scholar
Liu, H., Piao, C., Wang, L., Shin, S., Chung, Y., and Shu, Q. 2007. Biocontrol of Monochamus alternatus by Beauveria bassiana and Scleroderma guani . Scientia Silvae Sinicae, 43: 64–68.Google Scholar
Pilz, C., Enkerli, J., Wegensteiner, R., and Keller, S. 2011. Establishment and persistence of the entomopathogenic fungus Metarhizium anisopliae in maize fields. Journal of Applied Entomology, 135: 393–403.CrossRefGoogle Scholar
Samson, R.A., Evans, H.C., and Latge, J.P. 1988. Atlas of entomopathogenic fungi. Springer-Verlag, Berlin, Germany.CrossRefGoogle Scholar
Shimazu, M., Tsuchiya, D., Sato, H., and Kushida, T. 1995. Microbial control of Monochamus alternatus Hope (Coleoptera: Cerambycidae) by application of nonwoven fabric strips with Beauveria bassiana (Deuteromycotina: Hyphomycetes) on infested tree trunks. Applied Entomology and Zoology, 30: 207–213.CrossRefGoogle Scholar
Sutherland, J.R. 2008. A brief overview of the pine wood nematode and pine wilt disease in Canada and the United States. In Pine wilt disease. Edited by B. Zhao, K. Futai, J. Sutherland, and Y. Takeuchi. Springer, Tokyo, Japan. Pp. 13–17.CrossRefGoogle Scholar
Wang, S., Huang, Y., and Liu, Y. 2004. Screening and biological control of high virulent strains against Monochamus alternatus adults. Forest Pest and Disease, 23: 13–16.Google Scholar
Xia, C., Dind, D., and Liu, Y. 2005. Field trials against Spondylis buprestoides through combined use of non-woven fabric bands impregnated with Metarhizium anisopliae and an attractant. Journal of Anhui Agricultural University, 32: 419–422.Google Scholar
Xu, F. 2008. Recent advances in the integrated management of the pine wood nematode in China. In Pine wilt disease. Edited by B. Zhao, K. Futai, J. Sutherland, and Y. Takeuchi. Springer, Tokyo, Japan. Pp. 323–333.CrossRefGoogle Scholar
Xu, F., Xu, K., Xie, C., Zhang., P., Shin, S., and Cheong, Y. 2008. Studies on Scleroderma guani to control the pine sawyer beetle, Monochamus alternatus . In Pine wilt disease: a worldwide threat to forest ecosystems. Edited by M. Mota and P. Viera. Springer, Dordrecht, The Netherlands. Pp. 379–387.CrossRefGoogle Scholar
Yang, Z. 2004. Advance in bio-control researches of the important forest insect pests with natural enemies in China. Chinese Journal of Biological Control, 20: 221–227.Google Scholar
Yang, Z. and Michael, T.S. 2001. Investigations of natural enemies for biocontrol of Anoplophora glabripennis (Motsch.). In Proceedings U.S. Department of Agriculture interagency research forum on gypsy moth and other invasive species. Edited by S.L.C. Fosbroke and K.W. Gottschalk. Washington Post, http://www.uvm.edu/albeetle/research/biocontrol.html#NaturalEnemies, Annapolis, Maryland, United States of America. Pp. 139–141.Google Scholar
Yao, W. and Yang, Z. 2008. Studies on biological control of Anoplophora glabrennis (Coleoptera: Cerambycidae) with a parasitoid, Sclerodermus guani (Hymenoptera: Bethylidae). Journal of Environmental Entomology, 30: 127–134.Google Scholar