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An integrative phylogenetic analysis of eastern Nearctic Leuctra (Plecoptera: Leuctridae), with an emphasis on the fauna of a southern Appalachian Highlands landscape

Published online by Cambridge University Press:  28 February 2022

Madeline L. Metzger Open the ORCID record for Madeline L. Metzger [Opens in a new window] ,
Scott A. Grubbs Open the ORCID record for Scott A. Grubbs [Opens in a new window]  and
Jarrett R. Johnson
Madeline L. Metzger*
Affiliation:
Department of Biology and Center of Biodiversity Studies, Western Kentucky University, Bowling Green, KY42101, United States of America
Scott A. Grubbs
Affiliation:
Department of Biology and Center of Biodiversity Studies, Western Kentucky University, Bowling Green, KY42101, United States of America
Jarrett R. Johnson
Affiliation:
Department of Biology and Center of Biodiversity Studies, Western Kentucky University, Bowling Green, KY42101, United States of America
*
*Corresponding author. Email: [email protected]
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Abstract

Leuctra Stephens, 1836 is the fourth most speciose genus of Plecoptera (Leuctridae) east of the Rocky Mountains with 31 recognised species, trailing only Isoperla Banks, 1906 (58), Allocapnia Claassen, 1928 (47), and Perlesta Banks, 1906 (34). Although Leuctra females are described in taxonomic literature, they are difficult to morphologically distinguish among regional congeners, and identifications are often made through inference only (i.e., presence of males). This is particularly problematic in the Appalachian Mountains of the United States of America, which host numerous Leuctra species. We sampled the stonefly fauna of Mount Mitchell, western North Carolina, United States of America, from April to October 2019. The mitochondrial cytochrome C oxidase subunit 1 gene was sequenced for 39 adult males and 239 adult females of Leuctra. This allowed us to confidently place species names on all of the latter individuals. Phylogenetic tree- and genetic distance–based methods consistently grouped females with males for nine recognised species. Two separate L. ferruginea (Walker, 1852) operational taxonomic units were recognised, albeit with low divergence values, and an additional undetermined Leuctra was identified based solely on females. Digital stereomicroscope images were taken from females of each species unit to identify variation among and between species. This approach allowed for a more robust assessment of regional biogeographic patterns.

Type
Research Paper
Information
The Canadian Entomologist , Volume 154 , Issue 1 , 2022 , e15
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Copyright
© The Author(s), 2022. Published by Cambridge University Press on behalf of the Entomological Society of Canada

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Footnotes

Subject editor: Lisa Lumley

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