Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-12-01T00:13:41.130Z Has data issue: false hasContentIssue false

THE USE OF CRYSTAL VIOLET FOR DETERMINING THE AGE OF SAWFLY COCOONS IN THE SOIL

Published online by Cambridge University Press:  31 May 2012

L. A. Lyons
Affiliation:
Forest Research Laboratory, Department of Fisheries and Forestry, Sault Ste. Marie, Ontario

Abstract

Dilute solutions of several basic dyes, especially Crystal Violet and Malachite Green, effectively marked cocoons of Neodiprion sertifer (Geoff.). In field experiments, 0.25% Crystal Violet applied to soil at 1.0 gallon per square foot of surface area dyed most N. sertifer cocoons recognizably and was not removed by natural leaching or by the water used in extracting cocoons from the soil. Cocoons spun later in treated soil became contaminated but could be recognized by the distribution of dye on detritus adhering to the surface. Dyeing permitted direct measurement of the density of current generation cocoons in late August, when they were visually indistinguishable from old ones.

Type
Articles
Copyright
Copyright © Entomological Society of Canada 1969

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Conn, H. J. 1946. Biological stains. 5th ed. Biotech. Publ., Geneva, N.Y.Google Scholar
Heron, R. J. 1968. Vital dyes as markers for behavioural and population studies of the larch sawfly, Pristiphora erichsonii (Hymenoptera: Tenthredinidae). Can. Ent. 100: 470475.CrossRefGoogle Scholar
Lyons, L. A. 1964. The spatial distribution of two pine sawflies and methods of sampling for the study of population dynamics. Can. Ent. 96: 13731407.CrossRefGoogle Scholar