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STUDIES ON TECHNIQUES FOR THE XENIC AND ASEPTIC REARING OF THE EUROPEAN PINE SHOOT MOTH, RHYACIONIA BUOLIANA (LEPIDOPTERA: OLETHREUTIDAE)12

Published online by Cambridge University Press:  31 May 2012

R. H. Ross Jr. ,
R. E. Monroe  and
J. W. Butcher
R. H. Ross Jr.
Affiliation:
Department of Entomology, Michigan State University, East Lansing
R. E. Monroe
Affiliation:
Department of Entomology, Michigan State University, East Lansing
J. W. Butcher
Affiliation:
Department of Entomology, Michigan State University, East Lansing
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Abstract

A preliminary study to define a laboratory diet on which to rear the larvae of Rhyacionia buoliana (Schiff.) was made. The rearing vials were very important and gaseous exchange was essential for proper rearing and moisture levels. It was found that wheat germ appeared to contain essential components or balance of nutrients necessary for rearing this insect. Ascorbic acid also appeared to be an important component either for its nutritional value or as an antioxidant protecting other labile dietary components. It also appeared, because of the feeding habits of this insect and the length of its life cycle, that the larvae may have to be periodically transferred to new diet formulations to insure the presence of changing necessary nutrient(s). Aseptic rearing of the larvae seemed to be feasible. It was found that eggs, surface sterilized in 0.1% hypochlorite solution for 10–15 minutes, could be aseptically introduced into containers without appreciable decrease in egg hatch; however, sustained asepsis was difficult to maintain in the rearing vials employed.

Type
Articles
Information
The Canadian Entomologist , Volume 103 , Issue 10 , October 1971 , pp. 1449 - 1454
Copyright
Copyright © Entomological Society of Canada 1971

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