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Slow or stepped rewarming after acute low-temperature exposure does not improve survival of Drosophila melanogaster larvae

Published online by Cambridge University Press:  02 April 2012

Brent J. Sinclair  and
Arun Rajamohan
Brent J. Sinclair*
Affiliation:
Department of Biology, The University of Western Ontario, London, Ontario, Canada N6A 5B7
Arun Rajamohan
Affiliation:
Department of Biology, The University of Western Ontario, London, Ontario, Canada N6A 5B7
*
1Corresponding author (e-mail: [email protected]).
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Abstract

We tested the hypothesis that slow rewarming would improve the ability of Drosophila melanogaster Meigen (Diptera: Drosophilidae) larvae to survive acute low-temperature exposure. Four larval stages (1st, 2nd, and 3rd instars, including wandering-stage 3rd instars) of four wild-type strains were exposed to –7 °C for periods of time expected to result in 90% mortality. Larvae were then directly transferred to their rearing temperature (21 °C) or returned to this temperature either in a stepwise fashion (pausing at 0 and 15 °C) or by slow warming at 1 or 0.1 °C/min. We observed a reduced rapid cold-hardening effect and no general increase in survival of acute chilling in larvae rewarmed in a stepwise or slow fashion, and we hypothesize that slow rewarming may result in accumulation of chill injuries.

Résumé

Nous avons vérifié l’hypothèse qu’un réchauffement lent améliorerait les taux de survie chez les larves de Drosophila melanogaster Meigen (Diptera: Drosophilidae) après leur exposition à une période aiguë de froid. Les larves de quatre stades (1er, 2e, et 3e, incluant les larves errantes du stade 3) et de quatre souches de type sauvage furent exposées à une température de –7 °C pendant une période pour laquelle il était estimé qu’un taux de mortalité d’environ 90 % serait atteint. Les larves furent ensuite soit directement transférées à un régime de 21 °C, soit retournées à cette température par étapes (avec pauses à 0 et 15 °C) ou par réchauffement graduel d’un taux de 1 ou 0,1 °C/min. Nous avons observé une baisse de l’acclimatation rapide au froid ainsi que l’absence d’une augmentation de la survie des larves réchauffées soit graduellement ou par étapes. Ainsi, il est proposé qu’un réchauffement lent et par étapes résultera en l’accumulation de blessures associées au refroidissement.

Type
Articles
Information
The Canadian Entomologist , Volume 140 , Issue 3 , June 2008 , pp. 306 - 311
Copyright
Copyright © Entomological Society of Canada 2008

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